Sulfonated PEEK-statins delivery system enhances bone repair in mice with skull defect

Dan LYU; Yingxiao SUN; Liping OUYANG; Ling LI; Luping WANG; Yun LIAO

doi:10.16016/j.1000-5404.202005009

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Sulfonated PEEK-statins delivery system enhances bone repair in mice with skull defect

更新时间：2026-01-09

- Sulfonated PEEK-statins delivery system enhances bone repair in mice with skull defect
- Journal of Third Military Medical University Vol. 42, Issue 19, Pages: 1907-1912(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(81403029);the General Program of Shanghai Committee of Science and Technology(19ZR1449100)
- DOI：10.16016/j.1000-5404.202005009
  CLC：
- Received：03 May 2020，
  
  Published：15 October 2020
- 稿件说明：
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Dan LYU, Yingxiao SUN, Liping OUYANG, et al. Sulfonated PEEK-statins delivery system enhances bone repair in mice with skull defect[J]. Journal of Third Military Medical University, 2020, 42(19): 1907-1912.
DOI：

Dan LYU, Yingxiao SUN, Liping OUYANG, et al. Sulfonated PEEK-statins delivery system enhances bone repair in mice with skull defect[J]. Journal of Third Military Medical University, 2020, 42(19): 1907-1912. DOI： 10.16016/j.1000-5404.202005009.

摘要

Abstract

Objective

To explore the effect of sulfonated poly-ether-ether-ketone (SPEEK)-statin delivery system on the repair of bone defect

H-type blood vessels and bone formation.

Methods

The mouse model of skull defect was established

SPEEK-pravastatin (SPEEK-Pra) and sulfonated SPEEK-simvastatin (SPEEK-Sim) delivery system were constructed

respectively. Then the samples with different concentration gradients (SPEEK 0.16 mg/mL

SPEEK -Pra 0.16 mg/mL

SPEEK -Sim 0.55 mg/mL

SPEEK -Sim 1.1 mg/mL

SPEEK -Sim 2.2 mg/mL) were implanted into the skull defect of mice

respectively. After 8 weeks

the skull samples were collected

and micro-CT scanning was used to observe the bone regeneration around of the defects

and CD31 and EMCN immunofluorescent staining was utilized to label H-type blood vessels.

Results

SPEEK can be used as a payload for statins. Micro-CT results showed that the thickness of skull were significantly larger in SPEEK-sim 1.1 mg/mL and SPEEK-sim 0.55 mg /mL groups than the other 3 groups

with that of the SPEEK group (without drug loading) lowest. Immunofluorescence assay found that the double-positive area was clearly visible in the SPEEK-sim 1.1 mg /mL group

but not observed in the SPEEK group and the SPEEK-pra 0.16 mg/mL group.

Conclusion

SPEEK-statin sustained-release system optimizes the osteogenic properties of SPEEK

which may be related to the protective effect of H blood vessel.

关键词

Keywords

references

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