Protective effect of pericytes against vascular leakage in rats with hemorrhagic shock

Shuangshuang HE; Yu ZHU; Henan ZHOU; Hongchen WANG; Tao LI; Liangming LIU

doi:10.16016/j.1000-5404.202004227

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Protective effect of pericytes against vascular leakage in rats with hemorrhagic shock

更新时间：2026-01-09

- Protective effect of pericytes against vascular leakage in rats with hemorrhagic shock
- Journal of Third Military Medical University Vol. 42, Issue 19, Pages: 1882-1889(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Key Program of National Natural Science Foundation of China(81830065)
- DOI：10.16016/j.1000-5404.202004227
  CLC：
- Received：21 April 2020，
  
  Published：15 October 2020
- 稿件说明：
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Shuangshuang HE, Yu ZHU, Henan ZHOU, et al. Protective effect of pericytes against vascular leakage in rats with hemorrhagic shock[J]. Journal of Third Military Medical University, 2020, 42(19): 1882-1889.
DOI：

Shuangshuang HE, Yu ZHU, Henan ZHOU, et al. Protective effect of pericytes against vascular leakage in rats with hemorrhagic shock[J]. Journal of Third Military Medical University, 2020, 42(19): 1882-1889. DOI： 10.16016/j.1000-5404.202004227.

摘要

Abstract

Objective

To investigate the protective effect of pericytes (PCs) against vascular leakage in rats with hemorrhagic shock.

Methods

Sixty-four 12- to 24-week-old SD rats weighing 200~220 g were randomized equally into sham-operated group

hemorrhagic shock group (HS group)

Ringer's lactate solution treatment group (LR group)

and PC treatment group (PC group). In the latter 3 groups

rat models of hemorrhagic shock were established by lowering the blood pressure to 30 mmHg

which was maintained for 3 h. Tissue samples were collected 12 h after corresponding treatment for determination of the number of pericytes

vascular permeability of the pulmonary vessels and the mesenteric microvessels

and expressions of zonula occludens 1 (ZO-1) and VE-cadherin.

Results

The vascular permeability was increased (

0.05) and the number of pericytes and expressions of ZO-1 and VE-cadherin were decreased significantly (

0.05) in rats with hemorrhagic shock compared with the sham-operated rats. At 12 h after LR treatment

the vascular permeability was slightly reduced

the tight junction between the endothelial cells was moderately improved and the expression levels of ZO-1 and VE-cadherin were mildly increased in rats with hemorrhagic shock. At 12 h after PC treatment

the number of pericytes in the pulmonary vein was increased

the cell morphology and tight junction between the endothelial cells were restored

and the expressions of ZO-1 and VE-cadherin were increased significantly to nearly the normal levels in rats with hemorrhagic shock (

0.05); the mesenteric microvascular permeability and vascular leakage were improved significantly after treatment with PCs.

Conclusion

PC infusion produces significantly protective effect against vascular leakage in rats with hemorrhagic shock.

关键词

Keywords

references

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