Characteristics of HPV infection and value of its combination with TCT in screening cervical lesions: report based on 14 962 women taking physical examination

Le LI; Rui WANG

doi:10.16016/j.1000-5404.202004176

您当前的位置：

首页 >

文章列表页 >

Characteristics of HPV infection and value of its combination with TCT in screening cervical lesions: report based on 14 962 women taking physical examination

Clinical Medicine | 更新时间：2026-01-09

- Characteristics of HPV infection and value of its combination with TCT in screening cervical lesions: report based on 14 962 women taking physical examination
- Journal of Third Military Medical University Vol. 42, Issue 15, Pages: 1548-1554(2020)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院健康管理科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202004176
  CLC： R181.32;R373.9;R737.33
- Received：16 April 2020，
  
  Revised：2020-05-17，
  
  Published：15 August 2020
- 稿件说明：
移动端阅览
Le LI, Rui WANG. Characteristics of HPV infection and value of its combination with TCT in screening cervical lesions: report based on 14 962 women taking physical examination[J]. Journal of Third Military Medical University, 2020, 42(15): 1548-1554.
DOI：

Le LI, Rui WANG. Characteristics of HPV infection and value of its combination with TCT in screening cervical lesions: report based on 14 962 women taking physical examination[J]. Journal of Third Military Medical University, 2020, 42(15): 1548-1554. DOI： 10.16016/j.1000-5404.202004176.

摘要

目的

探讨联合高危型HPV和TCT筛查宫颈病变的应用价值。

方法

选取同时进行21种HPV基因分型检测及宫颈液基细胞学(TCT)检测的14 962例女性体检者，分别对HPV感染率、亚型分布、年龄段分布，及不同TCT结果中高危型HPV的感染率、高危型HPV和TCT联合检测等情况进行统计学分析。

结果

14 962例健康体检者中，共检出HPV阳性者1 482例，总感染阳性率9.91%，所有21种亚型，包括13种高危型，8种低危型均被检出。其中52型(17.61%)感染率最高，43型(0.06%)感染率最低。高危型HPV感染率前3位的分别是52型(17.61%)、58型(14.89%)、16型(11.88%)，低危型HPV感染率前3位的是53型(9.89%)、CP8304(低危型，6.48%)、66型(3.58%)。单一感染率最高，为78.34%(1 161/1 482)，二重感染率13.70%(203/1 482)，三重及以上感染率7.96%(118/1 482)。在

25岁、25~35岁、36~45岁、46~55岁、

55岁5个年龄组中，HPV感染率依次为12.72%(22/173)、10.70%(306/2 861)、9.07%(454/5 008)、9.75%(540/5 541)、11.60%(160/1 379)，其中

25岁年龄组感染率最高，各年龄组HPV感染率比较差异有统计学意义(

0.05)。随着宫颈病变程度加重，高危型HPV感染率增高，其中HISL最高，为83.90%(99/118)，各TCT组间的高危型HPV感染率差异有统计学意义(

0.05)。TCT阳性共537例(3.59%)，其中ASC-US 273例，LSIL 146例，HISL 118例。高危型HPV阳性共1 184例(7.91%)，二者共为阳性280例(1.87%)，二者联合检测阳性共1 441例(9.63%)，联合检测与单一检测阳性检出率相比，差异有统计学意义(

0.05)。

结论

体检女性高危型HPV亚型前3位为52、58、16型，HPV联合TCT筛查可提高宫颈病变检出率。

Abstract

Objective

To explore the application value of high-risk human papillomavirus (HPV) infection combined with and ThinPrep cytology test (TCT) in screening of cervical lesions.

Methods

A total of 14 962 women who undergoing 21 HPV genotyping and cervical fluid based TCT detection at the same time in our hospital from October 2018 to October 2019 were enrolled in this study. The HPV infection rate

subtype distribution

age distribution

and the high-risk HPV infection rates in different TCT results

and combined detection of high-risk HPV and TCT were observed and analyzed statistically.

Results

Among them

1 482 cases were positive to HPV

with a total positive rate of 9.91%. All 21 subtypes

including 13 high-risk types and 8 low-risk types

were detected. The highest infection rate was HPV 52 (17.61%)

and the lowest was HPV 43 (0.06%). The top 3 subtypes of high-risk HP

V infection were HPV 52 (17.61%)

58 (14.89%)

and 16 (11.88%)

and those of low-risk were HPV 53 (9.89%)

CP8304 (6.48%)

and 66 (3.58%). The highest single infection rate was 78.34% (1 161/1 482)

double infection rate 13.70% (203/1 482)

and triple and above infection rate 7.96% (118/1 482). For the 5 age groups (

25~35

36~45

46~55 and

55 years)

the infection rate of HPV was 12.72% (22/173)

10.70% (306/2 861)

9.07% (454/5 008)

9.75% (540/5 541) and 11.60% (160/1 379)

respectively. The highest infection rate was in the

25-year group

and statistical differences were seen among the different age groups (

0.05). With the increasing severity of cervical lesions

the infection rate of high-risk HPV was elevated

and high-grade intraepithelial lesion (HISL) was the most common lesion (83.90%

99/118). There were significant difference in the infection rate of high-risk HPV among different TCT groups (

0.05). There were 537 cases of TCT positive to HPV infection (3.59%)

including 273 cases of atypical squamous cells of undetermined significance (ASC-US)

146 cases of low-grade squamous intraepithelial lesion (LSIL) and 118 cases of HISL. There were 1 184 cases (7.91%) positive to high-risk HPV

280 cases (1.87%) were positive to both 2 detection

and 1 441 cases (9.63%) were positive to combined detection. The difference between the positive rate of combined detection and single detection was statistically significant (

0.05).

Conclusion

The top 3 high-risk HPV subtypes in female physical examiners are 52

58 and 16. Combined detection of HPV subtyping and TCT can improve the detection rate of cervical lesions.

关键词

Keywords

references

耿建祥, 黄华艺, 刘建华, 等.HPV感染疾病相关问题专家共识(2017)[J].医学研究生学报, 2017, 30(12):1238-1241.DOI: 10.16571/j.cnki.1008-8199.2017.12.002.

GENG J X, HUANG H Y, LIU J H, et al .Expert consensus on HPV infection and HPV-associated diseases(2017)[J ] . J Med Postgra, 2017, 30(12): 1238-1241. DOI:10.16571/j.cnki.1008-8199.2017.12.002.

范丰田, 安百芬, 袁启霞.子宫颈人乳头瘤病毒感染与宫颈癌的相关性[J].中华实验和临床感染病杂志(电子版), 2017, 11(1): 81-84. DOI:10.3877/cma.j.issn.1674-1358.2017.01.018.

FAN F T, AN B F, YUAN Q X. Correlation of cervical human papillomavirus infection and cervical cancer[J]. Chin J Exp Clin Infect Dis (Electr Ed), 2017, 11(1): 81-84. DOI:10.3877/cma.j.issn.1674-1358.2017.01.018.

CHEN W, ZHENG R, BAADE P D, et al .Cancer statistics in China, 2015[J ] . CA Cancer J Clin, 2016, 66(2):115-132.DOI: 10.3322/caac.21338.

HAGHSHENAS M R, MOUSAVI T, KHERADMAND M, et al . Efficacy of human papillomavirus L1 protein vaccines (Cervarix and Gardasil) in reducing the risk of cervical intraepithelial neoplasia: a meta-analysis[J ] . Int J Prev Med, 2017, 8:44. DOI:10.4103/ijpvm.IJPVM_324_16.

KUKIMO I. Human papillomavirus carcinogenesis mediated by APOBEC mutagenesis[J]. Yakugaku Zasshi, 2019, 139(1):75-79.DOI:10.1248/yakushi.18-00164-3.

刘佳欣, 吴方银, 梅榕, 等.成都市武侯区妇女高危型人乳头瘤病毒的流行病学研究[J].实用临床医药杂志, 2018, 22(23):95-97, 101. DOI:10.7619/jcmp.201823028.

LIU J X, WU F Y, MEI R, et al .Epidemiological research on women with high-risk human papillomavirus in Wuhou district of Chengdu city[J ] . J Clin Med Pract, 2018, 22(23):95-97, 101. DOI:10.7619/jcmp.201823028.

余艳琴, 富诗岚, 徐慧芳, 等.中国大陆女性体检人群中人乳头瘤病毒型别感染率及九价疫苗中HPV各型别分布的系统评价[J].肿瘤预防与治疗, 2019, 32(2):103-113.

YU Y Q, FU S L, XU H F, et al .Genotype-specific prevalence and distribution of cervical human papillomavirus in women undergoing health check-ups in mainland China:a systematic review based on population-based studies[J ] . J Cancer Control Treat, 2019, 32(2): 103-113.

马成, 莫殿军, 崔智慧, 等.赤峰地区2488例女性人乳头瘤病毒感染特征分析[J].国际检验医学杂志, 2020, 41(2): 239-241. DOI:10.3969/j.issn.1673-4130.2016.21.038.

MA C, MO D J, CUI Z H, et al . Analysis of the characteristics of human papillomavirus infection in 2488 women in Chifeng area[J ] . Int J Lab Med, 2020, 41(2): 239-241. DOI:10.3969/j.issn.1673-4130.2016.21.038.

余娟平, 魏琦, 王倩倩, 等.安徽地区17160例健康体检女性人乳头瘤病毒感染状况及基分型[J].中华实验和临床感染病杂志(电子版), 2019, 13(5):389-395.DOI:10.3877/cma.j.issn.1674-1358.2019.05.008.

YU J P, WEI Q, WANG Q Q, et al .Genotypes distribution of human papillomavirus in infected cervical tissue from women for physical examination in Anhui area[J ] .Chin J Exp Clin Infect Dis (Electr Ed), 2019, 13(5): 389-395.DOI:10.3877/cma.j.issn.1674-1358.2019.05.008.

彭蘡, 徐燕, 康厚纯, 等.溧阳地区HPV感染情况及基因亚型分析[J].中国继续医学教育, 2018, 10(5): 62-64. DOI:10.3969/j.issn.1674-9308.2018.05.028.

PENG Y, XU Y, KANG H C, et al . Prevalence and genotype distribution of HPV infection in Liyang City[J ] . China Continu Med Educ, 2018, 10(5): 62-64. DOI:10.3969/j.issn.1674-9308.2018.05.028.

赵佳, 何玉霞, 杜利君.四川南充地区3762例女性高危型人乳头瘤病毒感染结果分析[J].中国计划生育和妇产科, 2018, 10(8): 94-96. DOI:10.3969/j.issn.1674-4020.2018.08.25.

ZHAO J, HE Y X, DU L J. Analysis of 3762 women with high-risk HPV infection in Nanchong area, Sichuan[J]. Chin J Fam Plan Gynecotokol, 2018, 10(8): 94-96. DOI:10.3969/j.issn.1674-4020.2018.08.25.

刘宓, 王伟, 陆毅, 等.贵州省肿瘤医院2010-2016年妇女HPV感染亚型分布情况分析[J].现代预防医学, 2018, 45(7): 1207-1210.

LIU M, WANG W, LU Y, et al . Distribution of HPV subtypes in women in cancer hospital of Guizhou university, 2010-2016[J ] . Mod Prev Med, 2018, 45(7): 1207-1210.

WANG Y Y, WANG S H, SHEN J H, et al . Genotype distribution of human papillomavirus among women with cervical cytological abnormalities or invasive squamous cell carcinoma in a high-incidence area of esophageal carcinoma in China[J ] . Biomed Res Int, 2016, 2016: 1256384. DOI:10.1155/2016/1256384.

YU J H, SHI W W, ZHOU M Y, et al . Genetic variability and oncogenic risk association of human papillomavirus type 58 E6 and E7 genes in Taizhou area, China[J ] . Gene, 2019, 686:171-176. DOI: 10.1016/j.gene.2018.11.066.

杨丽, 王静, 马彩玲.预防性HPV疫苗预防宫颈癌有效性和安全性Meta分析[J].中华肿瘤防治杂志, 2015, 22(9):719-727.

YANG L, WANG J, MA C L. Efficacy and safety of prophylactic human papilloma virus vaccine in cervical cancer prevention:a meta analysis[J]. Chin J Cancer Prev Treat, 2015, 22(9):719-727.

曾华钰琪. HPV疫苗研究进展及认知度、接受度现状分析[J].实用妇科内分泌电子杂志, 2019, 6(20): 16. DOI:10.16484/j.cnki.issn2095-8803.2019.20.010.

ZENG H Y Q. Research progress of HPV vaccine and analysis of its recognition and acceptance[J]. Electr J Pract Gynecol Endocrinol, 2019, 6(20): 16. DOI:10.16484/j.cnki.issn2095-8803.2019.20.010.

郑家凤, 周燕.HPV多重感染与宫颈癌及癌前病变发生、发展的相关性[J].实用癌症杂志, 2019, 34(7): 1107-1109.DOI:10.3969/j.issn.1001-5930.2019.07.018.

ZHENG J F, ZHOU Y.Study of relationship between HPV multiple infection and occurrence and development of cervical Cancer and precancerous lesions[J]. Pract J Cancer, 2019, 34(7): 1107-1109. DOI:10.3969/j.issn.1001-5930.2019.07.018.

白焕焕, 蔡江义.女性下生殖道HPV感染与宫颈上皮内瘤变及宫颈癌关系的病理研究[J].现代肿瘤医学, 2017, 25(4): 617-619.

BAI H H, CAI J Y. Relation of female lower genital tract HPV infection and cervical intraepithelial neoplasia and cervical cancer[J]. J Mod Oncol, 2017, 25(4): 617-619.

吴凯.福建地区女性HPV感染的流行病学特征与宫颈早期病变的关系[J].临床合理用药杂志, 2019, 12(22): 165-167. DOI:10.15887/j.cnki.13-1389/r.2019.22.094.

WU K. The relationship between the epidemiological characteristics of HPV infection and early cervical lesions in Fujian women[J]. Chin J Clin Ration Drug Use, 2019, 12(22): 165-167. DOI:10.15887/j.cnki.13-1389/r.2019.22.094.

FLEMING K, SIMMONS V N, CHRISTY S M, et al . Educating hispanic women about cervical cancer prevention: feasibility of a promotora-led charla intervention in a farmworker community[J ] . Ethn Dis, 2018, 28(3): 169-176. DOI:10.18865/ed.28.3.169.

张驰东, 高燕, 缪应新, 等.上海地区老年女性人乳头状瘤病毒感染型别分布特征[J].老年医学与保健, 2018(4): 397-400. DOI:10.3969/j.issn.1008-8296.2018.04.011.

ZHANG C D, GAO Y, MIAO Y X, et al . Type and distribution characteristics of HPV in female elderly in Shanghai[J ] . Geriatr Health Care, 2018(4): 397-400. DOI:10.3969/j.issn.1008-8296.2018.04.011.

陶志梅, 潘敏, 俞美娟, 等.高危型人乳头瘤病毒感染联合液基薄层细胞检测对宫颈癌及宫颈癌前病变筛查与随访的临床意义[J].中华医院感染学杂志, 2017, 27(10): 2340-2343. DOI:10.11816/cn.ni. 2017-163607.

TAO Z M, PAN M, YU M J, et al . Clinical significance of detection of high-risk human papillomavirus infection combined with liquid-based thin-layer cytology test in screening, follow-up of cervical cancer and cervical precancerous lesions[J ] . Chin J Nosocomiol, 2017, 27(10): 2340-2343.DOI:10.11816/cn.ni.2017-163607.

杨丽华, 冷天艳, 张琴, 等.杂交捕获定量分型技术检测高危型人乳瘤病毒在子宫颈癌筛查中的应用[J].中国妇产科临床杂志, 2016, 17(6): 542-543. DOI:10.13390/j.issn.1672-1861.2016.06.018.

YANG L H, LENG T Y, ZHANG Q, et al . Application of hybridized capture and quantitative typing in screening of cervical cancer[J ] . Chin J Clin Obstet Gynecol, 2016, 17(6): 542-543. DOI:10.13390/j.issn. 1672-1861.2016.06.018.

林兵英, 罗晓燕, 方周宾, 等.液基薄层细胞检查联合HPV-DNA分型检测在宫颈癌筛查中的价值对比[J].中国医药科学, 2019, 9(13): 114-117.

LIN B Y, LUO X Y, FANG Z B, et al . Comparison of values of thin-layer cytological test combined with HPV-DNA genotyping testin cervical cancer screening[J ] . China Med Pharm, 2019, 9(13): 114-117.

彭晓琴, 蔡和伶.人乳头瘤病毒分型检测在宫颈高度病变筛查中的应用价值[J].临床合理用药杂志, 2019, 12(9): 170-171, 174. DOI:10.15887/j.cnki.13-1389/r.2019.09.081.

PENG X Q, CAI H L. The application value of human papillomavirus typing in cervical high lesion screening[J]. Chin J Clin Ration Drug Use, 2019, 12(9): 170-171, 174. DOI:10.15887/j.cnki.13-1389/r.2019.09.081.

US Preventive Services Task Force, CURRY S J, KRIST A H, et al . Screening for cervical cancer: US preventive services task force recommendation statement[J ] . JAMA, 2018, 320(7): 674-686. DOI:10.1001/jama.2018.10897.

赵君, 赵媛.液基细胞学与HPV分型联合检测对早期宫颈癌的筛查意义[J].实用癌症杂志, 2018, 33(1): 143-146. DOI:10.3969/j.issn.1001-5930.2018.01.044.

ZHAO J, ZHAO Y. Significance of liquid based cytology and HPV typing in the screening of early cervical cancer[J]. Pract J Cancer, 2018, 33(1): 143-146. DOI:10.3969/j.issn.1001-5930.2018.01.044.

梁月兰, 赵雪, 耿建祥, 等.宫颈ASC-US、LSIL和HSIL中HPV感染型别分布的研究[J].国际检验医学杂志, 2018, 39(1): 59-62.

LIANG Y L, ZHAO X, GENG J X, et al . Study on HPV infection types distribution in ASC-US, LSIL and HSIL of uterine cervix[J ] . Int J Lab Med, 2018, 39(1): 59-62.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Advances in deep learning and MRI co-assisted genotyping of brain gliomas

Genotyping of extended spectrum beta lactamases of 33 Escherichia coli strains from several hospitals of Guiyang City

Correlation of vaginal intraepithelial neoplasia with high-risk HPV, liquid-based cytology and cervical lesions in patients without hysterectomy

Investigation of human papillomavirus infections in cervical epithelial cells in Chongqing: report based on 32 882 women

Genotyping of 101 Mycobacterium tuberculosis isolates from pediatrics tuberculosis in Chongqing by multiple locus VNTR analysis

Related Author

CHEN Junshan

CUI Shaoguo

Fang CHEN

Jun YU

Yaqiong DONG

Yunfen CHEN

钱睿亚

王晓丹

Related Institution

College of Computer and Information Science， Chongqing Normal University

Department of Respiratory Diseases, the Sixth Hospital of Guiyang

Department of Respiratory Diseases, Affiliated Hospital of Guizhou Medical University

⁰