Galectin-3 regulates proliferation of glomerular mesangial cells under high glucose condition

Ziyue LIN; Panyang ZHANG; Xinyu CHONG; Yan SUN; Rui PENG; Zheng ZHANG

doi:10.16016/j.1000-5404.202004076

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Galectin-3 regulates proliferation of glomerular mesangial cells under high glucose condition

更新时间：2026-01-09

- Galectin-3 regulates proliferation of glomerular mesangial cells under high glucose condition
- Journal of Third Military Medical University Vol. 42, Issue 19, Pages: 1913-1919(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81970702)
- DOI：10.16016/j.1000-5404.202004076
  CLC：
- Received：08 April 2020，
  
  Published：15 October 2020
- 稿件说明：
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Ziyue LIN, Panyang ZHANG, Xinyu CHONG, et al. Galectin-3 regulates proliferation of glomerular mesangial cells under high glucose condition[J]. Journal of Third Military Medical University, 2020, 42(19): 1913-1919.
DOI：

Ziyue LIN, Panyang ZHANG, Xinyu CHONG, et al. Galectin-3 regulates proliferation of glomerular mesangial cells under high glucose condition[J]. Journal of Third Military Medical University, 2020, 42(19): 1913-1919. DOI： 10.16016/j.1000-5404.202004076.

摘要

Abstract

Objective

To investigate the effect and underlying mechanism of galectin-3 (Gal-3) on the proliferation of mesangial cells (MCs) cultured under high glucose condition.

Methods

Real-time quantitative PCR (qRT-PCR) was used to detect the expression of Gal-3 in normal and diabetic nephropathy (DN) mouse kidney tissues and MCs under low and high glucose conditions. Gal-3 overexpression plasmid and siRNA were constructed and synthesized. qRT-PCR and Western blotting were used to detect the transfection efficiency. And Gal-3 overexpression plasmid or siRNA was transfected into MCs under low and high glucose condition

respectively. Further

5-Ethynyl-2′-deoxyuridine (EdU) was used to detect the cell proliferation ability

and Western blot assay was used to measure the expression of Mek and phosphorylated Mek after overexpression or downexpression of Gal-3 in MCs.

Results

qRT-PCR results showed that Gal-3 expression was significantly higher in the kidney tissues of DN mice than those of the normal mice(

0.01)

and so was the expression level in MCs under high glucose condition when compared with the cells under low glucose condition (

0.01). In addition

overexpression of Gal-3 resulted in enhanced proliferation ability of MCs (

0.01) and the up-regulation of phosphorylated Mek (

0.01). While

Gal-3 silencing induced the opposite results in the proliferation ability (

0.01) and phosphorylated Mek expression (

0.001).

Conclusion

Gal-3 is significantly up-regulated in DN

and may affect the proliferation of MCs by regulating Mek phosphorylation

and thus plays an important regulatory role in DN.

关键词

Keywords

references

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