Antibacterial activity and mechanism of antimicrobial peptide ET12 against Gram-positive bacteria

Qian YUAN; Wei FENG; Yu WANG; Pu YAO; Fengjun SUN

doi:10.16016/j.1000-5404.202003346

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Antibacterial activity and mechanism of antimicrobial peptide ET12 against Gram-positive bacteria

Pharmacy | 更新时间：2026-01-09

- Antibacterial activity and mechanism of antimicrobial peptide ET12 against Gram-positive bacteria
- Journal of Third Military Medical University Vol. 42, Issue 15, Pages: 1567-1572(2020)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院药剂科
- 作者简介：
- 基金信息：
  
  the National Key R & D Plan of China(2017YFC1200400)
- DOI：10.16016/j.1000-5404.202003346
  CLC： R378;R965;R977.6
- Received：26 March 2020，
  
  Revised：2020-05-09，
  
  Published：15 August 2020
- 稿件说明：
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Qian YUAN, Wei FENG, Yu WANG, et al. Antibacterial activity and mechanism of antimicrobial peptide ET12 against Gram-positive bacteria[J]. Journal of Third Military Medical University, 2020, 42(15): 1567-1572.
DOI：

Qian YUAN, Wei FENG, Yu WANG, et al. Antibacterial activity and mechanism of antimicrobial peptide ET12 against Gram-positive bacteria[J]. Journal of Third Military Medical University, 2020, 42(15): 1567-1572. DOI： 10.16016/j.1000-5404.202003346.

摘要

目的

研究抗菌肽ET12的抗菌活性及机制。

方法

通过微量肉汤稀释法检测抗菌肽ET12对临床常见菌株的最低抑菌浓度(MIC)，激光共聚焦显微镜观察抗菌肽ET12对表皮葡萄球菌生物膜形成和细胞膜通透性的影响，透射电镜观察抗菌肽ET12对表皮葡萄球菌细胞形态的影响，利用脱纤维绵羊血检测抗菌肽ET12的溶血活性，利用CCK-8试剂盒检测抗菌肽ET12对细胞的毒性。

结果

抗菌肽ET12对革兰阳性菌特别是表皮葡萄球菌具有良好的抗菌活性，MIC值在8~64 μg/mL之间。ET12能够有效抑制表皮葡萄球菌生物膜的形成及破坏细菌细胞形态，增强细胞膜通透性。在有效抗菌浓度范围内，ET12对绵羊红细胞的溶血率小于2.5%，RAW264.7细胞存活率大于80%。

结论

抗菌肽ET12对革兰阳性菌具有较强的抗菌活性，细胞膜可能是其抗菌作用靶点，且溶血活性和细胞毒性较低。

Abstract

Objective

To study the antibacterial activity and mechanism of antimicrobial peptide ET12.

Methods

The minimal inhibitory concentration (MIC) of ET12 on clinical common strains was determined by broth dilution method. The effect of ET12 on the biofilm formation and membrane permeability of

Staphylococcus epidermidis

(

S. epidermidis

) was observed by confocal laser microscopy. The effect of ET12 on the cell morphology was observ

ed by transmission electron microscopy. Defibrillation sheep blood was used to detect the hemolytic activity of ET12. Cell counting Kit-8 assay was used to detect the cytotoxicity of ET12.

Results

ET12 had good antibacterial activity against Gram-positive bacteria

especially

S. epidermidis

. The MIC values of ET12 against Gram-positive bacteria were between 8 and 64 μg/mL. ET12 could inhibit the biofilm formation of

S. epidermidis

damage the bacterial morphology and enhance cell membrane permeability. Within the effective concentration ranges of antibacterial activity

the hemolysis rate of ET12 on sheep red blood cells was less than 2.5%

and the survival rate of RAW264.7 cells was above 80%.

Conclusion

ET12 has strong antibacterial activity against Gram-positive bacteria

with the cell membrane as target

and has low hemolytic activity and cytotoxicity.

关键词

Keywords

references

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