MiR-186-5p negatively regulates CDCA3 to improve proliferation and inhibit apoptosis of islet β cells induced by high fat and high glucose

Tie ZHAI; Xuepin TIAN; Fengjie HAO; Yanqiu ZHANG; Xiaojun WANG

doi:10.16016/j.1000-5404.202003247

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MiR-186-5p negatively regulates CDCA3 to improve proliferation and inhibit apoptosis of islet β cells induced by high fat and high glucose

更新时间：2026-01-09

- MiR-186-5p negatively regulates CDCA3 to improve proliferation and inhibit apoptosis of islet β cells induced by high fat and high glucose
- Journal of Third Military Medical University Vol. 42, Issue 19, Pages: 1930-1937(2020)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202003247
  CLC：
- Received：19 March 2020，
  
  Published：15 October 2020
- 稿件说明：
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Tie ZHAI, Xuepin TIAN, Fengjie HAO, et al. MiR-186-5p negatively regulates CDCA3 to improve proliferation and inhibit apoptosis of islet β cells induced by high fat and high glucose[J]. Journal of Third Military Medical University, 2020, 42(19): 1930-1937.
DOI：

Tie ZHAI, Xuepin TIAN, Fengjie HAO, et al. MiR-186-5p negatively regulates CDCA3 to improve proliferation and inhibit apoptosis of islet β cells induced by high fat and high glucose[J]. Journal of Third Military Medical University, 2020, 42(19): 1930-1937. DOI： 10.16016/j.1000-5404.202003247.

摘要

Abstract

Objective

To investigate whether miR-186-5p can regulate the proliferation and apoptosis of islet β-cells induced by high fat and high glucose through cell division cycle associated 3 protein (CDCA3).

Methods

Mouse pancreatic β-cell line NIT-1 was treated with high lipid and glucose to induce injury. CCK-8 assay and flow cytometry were employed to measure cell proliferation and apoptosis. The expression of Ki-67

PCNA

Bcl-2 and Bax at protein level was analyzed by Western blotting

while that of miR-186-5p and CDCA3 at mRNA level was determined by qRT-PCR. Bioinformatics and dual luciferase activity assay were used to predict and detect the targeting relationship between miR-186-5p and CDCA3. NIT-1 cells were transfected with miR-186-5p or si-CDCA3

and treated with high lipid and high glucose

and the effects on cell proliferation and apoptosis were observed.

Results

High lipid and high glucose significantl

y reduced the cell viability

protein levels of Ki-67

PCNA and Bcl-2

and mRNA level of miR-186-5p expression in NIT-1 cells

while significantly increased the cell apoptotic rate and Bax protein expression

as well as CDCA3 expression at mRNA and protein levels (

0.05). MiR-186-5p negatively regulates CDCA3 as a target. Overexpression of miR-186-5p or knockdown of CDCA3 dramatically improved the cell viability

increased the expression of Ki-67

PCNA

and Bcl-2 at protein level

while remarkably decreased the apoptotic rate and Bax protein expression in NIT-1 cells after the treatment of high lipid and high glucose (

0.05).

Conclusion

ConclusionmiR-186-5p can negatively regulate the expression of CDCA3 gene

and thus promote the proliferation of islet β cells induced by high fat and high glucose and inhibit the apoptosis

so as to protect the islet β cells against the damage induced by high fat and high glucose.

关键词

Keywords

references

NG L C GUPTA M Transdermal drug delivery systems in diabetes management: a review Asian J Pharm Sci 2020 15 1 13 25 10.1016/j.ajps.2019.04.006 NG L C, GUPTA M. Transdermal drug delivery systems in diabetes management: a review[J]. Asian J Pharm Sci, 2020, 15(1): 13-25. DOI:10.1016/j.ajps.2019.04.006.

YE R S ONODERA T SCHERER P E Lipotoxicity and β cell maintenance in obesity and type 2 diabetes J. Endocr. Soc. 2019 3 3617 631 10.1210/js.2018-00372 YE R S, ONODERA T, SCHERER P E. Lipotoxicity and β cell maintenance in obesity and type 2 diabetes[J]. J. Endocr. Soc., 2019, 3(3): 617-631. DOI:10.1210/js.2018-00372.

MARTINEZ-SANCHEZ A NGUYEN-TU M S CEBOLA I MiR-184 expression is regulated by AMPK in pancreatic islets FASEB J 2018 32 5 2587 2600 10.1096/fj.201701100R MARTINEZ-SANCHEZ A, NGUYEN-TU M S, CEBOLA I, et al. MiR-184 expression is regulated by AMPK in pancreatic islets[J ] . FASEB J, 2018, 32(5): 2587-2600. DOI:10.1096/fj.201701100R.

WU D H LIANG H LU S N miR-124 suppresses pancreatic ductal adenocarcinoma growth by regulating monocarboxylate transporter 1-mediated cancer lactate metabolism Cell Physiol Biochem 2018 50 3 924 935 10.1159/000494477 WU D H, LIANG H, LU S N, et al. miR-124 suppresses pancreatic ductal adenocarcinoma growth by regulating monocarboxylate tra nsporter 1-mediated cancer lactate metabolism[J ] . Cell Physiol Biochem, 2018, 50(3): 924-935. DOI:10.1159/000494477.

MICHLEWSKI G CÁCERES J F Post-transcriptional control of miRNA biogenesis RNA 2019 25 1 1 16 10.1261/rna.068692.118 MICHLEWSKI G, CÁCERES J F. Post-transcriptional control of miRNA biogenesis[J]. RNA, 2019, 25(1): 1-16. DOI:10.1261/rna.068692.118.

NIGI L GRIECO G E VENTRIGLIA G MicroRNAs as regulators of insulin signaling: research updates and potential therapeutic perspectives in type 2 diabetes Int J Mol Sci 2018 19 12 3705 10.3390/ijms19123705 NIGI L, GRIECO G E, VENTRIGLIA G, et al. MicroRNAs as regulators of insulin signaling: research updates and potential therapeutic perspectives in type 2 diabetes[J ] . Int J Mol Sci, 2018, 19(12): 3705. DOI:10.3390/ijms19123705.

RAJAN S PANZADE G SRIVASTAVA A miR-876-3p regulates glucose homeostasis and insulin sensitivity by targeting adiponectin J Endocrinol 2018 239 1 1 17 10.1530/JOE-17-0387 RAJAN S, PANZADE G, SRIVASTAVA A, et al. miR-876-3p regulates glucose homeostasis and insulin sensitivity by targeting adiponectin[J ] . J Endocrinol, 2018, 239(1): 1-17. DOI:10.1530/JOE-17-0387.

郭润民刘畅吴子君糖尿病心肌病患者血清差异表达microRNA及作用的研究中国医药科学 2016 6 13 20 23 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgyykx201613006 郭润民, 刘畅, 吴子君, 等.糖尿病心肌病患者血清差异表达microRNA及作用的研究[J].中国医药科学, 2016, 6(13): 20-23.

GUO R M LIU C WU Z J Serum differentially expression microRNA profile and potential roles in diabetic cardiomyopathy patients China Med Pharm 2016 6 13 20 23 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zgyykx201613006 GUO R M, LIU C, WU Z J, et al. Serum differentially expression microRNA profile and potential roles in diabetic cardiomyopathy patients[J]. China Med Pharm, 2016, 6(13): 20-23.

胡朝恩 . Exenatide对高糖高脂诱导的NIT-1细胞损伤和炎症因子过表达的影响 [D ] . 泸州 : 四川医科大学 , 2015 . 胡朝恩. Exenatide对高糖高脂诱导的NIT-1细胞损伤和炎症因子过表达的影响[D].泸州: 四川医科大学, 2015.

HU C E . Effects of Exenatide on high glucose and high fat induced NIT-1 cell damage and overexpression of inflammatory factors [D ] . Luzhou : Sichuan Medical University , 2015 . HU C E. Effects of Exenatide on high glucose and high fat induced NIT-1 cell damage and overexpression of inflammatory factors[D]. Luzhou: Sichuan Medical University, 2015.

WANG W Y WANG J YAN M L MiRNA-92a protects pancreatic B-cell function by targeting KLF2 in diabetes mellitus Biochem Biophys Res Commun 2018 500 3 577 582 10.1016/j.bbrc.2018.04.097 WANG W Y, WANG J, YAN M L, et al. MiRNA-92a protects pancreatic B-cell function by targeting KLF2 in diabetes mellitus[J ] . Biochem Biophys Res Commun, 2018, 500(3): 577-582. DOI:10.1016/j.bbrc.2018.04.097.

SHEN Z Y JIANG H M HSU H T MicroRNA-127 inhibits cell proliferation via targeting Kif3b in pancreatic β cells Aging 2019 11 5 1342 1355 10.18632/aging.101835 SHEN Z Y, JIANG H M, HSU H T, et al. MicroRNA-127 inhibits cell proliferation via targeting Kif3b in pancreatic β cells[J ] . Aging, 2019, 11(5): 1342-1355. DOI:10.18632/aging.101835.

张萌欧阳礼辰龙漫桑白皮含药血清抑制高脂高糖诱导的胰岛β细胞凋亡的分子机制湖北中医药大学学报 2018 20 4 15 17 10.3969/j.issn.1008-987x.2018.04.03 张萌, 欧阳礼辰, 龙漫.桑白皮含药血清抑制高脂高糖诱导的胰岛β细胞凋亡的分子机制[J].湖北中医药大学学报, 2018, 20(4): 15-17. DOI:10.3969/j.issn.1008-987x.2018.04.03.

ZHANG M OUYANG L C LONG M Molecular mechanism of cortex mori radicis serum inhibiting apoptosis of islet β cells induced by palmitic acid and high glucose J Hubei Univ Chin Med 2018 20 4 15 17 10.3969/j.issn.1008-987x.2018.04.03 ZHANG M, OUYANG L C, LONG M. Molecular mechanism of cortex mori radicis serum inhibiting apoptosis of islet β cells induced by palmitic acid and high glucose[J]. J Hubei Univ Chin Med, 2018, 20(4): 15-17. DOI:10.3969/j.issn.1008-987x.2018.04.03.

TAN H Y ZHAO L lncRNA nuclear-enriched abundant transcript 1 promotes cell proliferation and invasion by targeting miR-186-5p/HIF-1α in osteosarcoma J Cell Biochem 2019 120 4 6502 6514 10.1002/jcb.27941 TAN H Y, ZHAO L. lncRNA nuclear-enriched abundant transcript 1 promotes cell proliferation and invasion by targeting miR-186-5p/HIF-1α in osteosarcoma[J]. J Cell Biochem, 2019, 120(4): 6502-6514. DOI:10.1002/jcb.27941.

LIU X ZHOU X CHEN Y miR-186-5p targeting SIX1 inhibits cisplatin resistance in non-small-cell lung cancer cells (NSCLCs) Neoplasma 2020 67 1 147 157 10.4149/neo_2019_190511N420 LIU X, ZHOU X, CHEN Y, et al. miR-186-5p targeting SIX1 inhibits cisplatin resistance in non-small-cell lung cancer cells (NSCLCs)[J ] . Neoplasma, 2020, 67(1): 147-157. DOI:10.4149/neo_2019_190511N420.

OUYANG Y M LI Y J HUANG Y G CircRNA circPDSS1 promotes the gastric cancer progression by sponging miR-186-5p and modulating NEK2 J Cell Physiol 2019 234 7 10458 10469 10.1002/jcp.27714 OUYANG Y M, LI Y J, HUANG Y G, et al. CircRNA circPDSS1 promotes the gastric cancer progression by sponging miR-186-5p and modulating NEK2[J ] . J Cell Physiol, 2019, 234(7): 10458-10469. DOI:10.1002/jcp.27714.

ZHANG Z Q ZHANG W MAO J S miR-186-5p functions as a tumor suppressor in human osteosarcoma by targeting FOXK1 Cell Physiol Biochem 2019 52 3 553 564 10.33594/000000039 ZHANG Z Q, ZHANG W, MAO J S, et al. miR-186-5p functions as a tumor suppressor in human osteosarcoma by targeting FOXK1[J ] . Cell Physiol Biochem, 2019, 52(3): 553-564. DOI:10.33594/000000039.

ZHU K SU Y L XU B MicroRNA-186-5p represses neuroblastoma cell growth via downregulation of Eg5 Am J Transl Res 2019 11 4 2245 2256 http://www.researchgate.net/publication/333246028_MicroRNA-186-5p_represses_neuroblastoma_cell_growth_via_downregulation_of_Eg5 ZHU K, SU Y L, XU B, et al. MicroRNA-186-5p represses neuroblastoma cell growth via downregulation of Eg5[J ] . Am J Transl Res, 2019, 11(4): 2245-2256.

LIU Y ZHENG W PAN Y Low expression of miR-186-5p regulates cell apoptosis by targeting toll-like receptor 3 in high glucose-induced cardiomyocytes J Cell Biochem 2019 120 6 9532 9538 10.1002/jcb.28229 LIU Y, ZHENG W, PAN Y, et al. Low expression of miR-186-5p regulates cell apoptosis by targeting toll-like receptor 3 in high glucose-induced cardiomyocytes[J ] . J Cell Biochem, 2019, 120(6): 9532-9538. DOI:10.1002/jcb.28229.

JIANG J M MO H L LIU C Inhibition of miR-186-5p contributes to high glucose-induced injury in AC16 cardiomyocytes Exp Ther Med 2018 15 1 627 632 10.3892/etm.2017.5445 JIANG J M, MO H L, LIU C, et al. Inhibition of miR-186-5p contributes to high glucose-induced injury in AC16 cardiomyocytes[J ] . Exp Ther Med, 2018, 15(1): 627-632. DOI:10.3892/etm.2017.5445.

ADAMS M N BURGESS J T HE Y W Expression of CDCA3 is a prognostic biomarker and potential therapeutic target in non-small cell lung cancer J Thorac Oncol 2017 12 7 1071 1084 10.1016/j.jtho.2017.04.018 ADAMS M N, BURGESS J T, HE Y W, et al. Expression of CDCA3 is a prognostic biomarker and potential therapeutic target in non-small cell lung cancer[J ] . J Thorac Oncol, 2017, 12(7): 1071-1084. DOI:10.1016/j.jtho.2017.04.018.

ZHANG Y YIN W CAO W CDCA3 is a potential prognostic marker that promotes cell proliferation in gastric cancer Oncol Rep 2019 41 4 2471 2481 10.3892/or.2019.7008 ZHANG Y, YIN W, CAO W, et al. CDCA3 is a potential prognostic marker that promotes cell proliferation in gastric cancer[J ] . Oncol Rep, 2019, 41(4): 2471-2481. DOI:10.3892/or.2019.7008.

ZHANG W LU Y X LI X M CDCA3 promotes cell proliferation by activating the NF-κB/cyclin D1 signaling pathway in colorectal cancer Biochem Biophys Res Commun 2018 500 2 196 203 10.1016/j.bbrc.2018.04.034 ZHANG W, LU Y X, LI X M, et al. CDCA3 promotes cell proliferation by activating the NF-κB/cyclin D1 signaling pathway in colorectal cancer[J ] . Biochem Biophys Res Commun, 2018, 500(2): 196-203. DOI:10.1016/j.bbrc.2018.04.034.

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