Role of NF-κB signaling pathway in treatment of intrauterine adhesions by human amniotic mesenchymal stem cells

Yating GOU; Wenwen ZHANG; Changjiang LI; Jie YU; Yanhua MAO; Benyuan WU; Yingfeng ZHANG; Xinpei CHEN; Jiayue HUANG; Congcong SUN; Jia WANG

doi:10.16016/j.1000-5404.202003065

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Role of NF-κB signaling pathway in treatment of intrauterine adhesions by human amniotic mesenchymal stem cells

更新时间：2026-01-09

- Role of NF-κB signaling pathway in treatment of intrauterine adhesions by human amniotic mesenchymal stem cells
- Journal of Third Military Medical University Vol. 42, Issue 11, Pages: 1101-1108(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Basic Research and Frontier Exploration Project of Yuzhong District, Chongqing(20190122);the Postgraduate Scientific Research and Innovation Project of Chongqing(CYS19195)
- DOI：10.16016/j.1000-5404.202003065
  CLC：
- Received：05 March 2020，
  
  Published：15 June 2020
- 稿件说明：
移动端阅览
Yating GOU, Wenwen ZHANG, Changjiang LI, et al. Role of NF-κB signaling pathway in treatment of intrauterine adhesions by human amniotic mesenchymal stem cells[J]. Journal of Third Military Medical University, 2020, 42(11): 1101-1108.
DOI：

Yating GOU, Wenwen ZHANG, Changjiang LI, et al. Role of NF-κB signaling pathway in treatment of intrauterine adhesions by human amniotic mesenchymal stem cells[J]. Journal of Third Military Medical University, 2020, 42(11): 1101-1108. DOI： 10.16016/j.1000-5404.202003065.

摘要

Abstract

Objective

To investigate the role of IκB-α/NF-κB signaling pathway in the treatment of intrauterine adhesions (IUA) by human amniotic mesenchymal stem cells (hAMSCs).

Methods

In vivo

experiment: 18 SD female rats were randomly divided into Sham group

IUA model group and hAMSCs group. The number of endometrial glands and the percentage of fibrotic area were calculated by HE staining and Masson staining

respectively. Immunohistochemistry was used to detect the expression levels of IκB-α (NF-κB inhibitor)

nuclear transfer factor P65

E-cadherin and vimentin in endometrial tissue.

In vitro

experiments: hAMSCs were induced by exogenous growth factors (10 ng/mL TGF-β1+EGF+PDGF-BB) and 10 nmol/L BAY11-7082 combined with above growth factors for 5 d

respectively

and the cells without treatment served as blank control. Fluorescence quantitative PCR was used to detect the expression levels of IκB-α

and cytokeratins CK-7 and CK-19.

Results

In vivo

experiment: The expression levels of IκB-α

P65 and vimentin was significant higher

while that of E-

cadherin was obviously lower in the model group than the Sham group (all

0.05); the expression levels of IκB-α

P65 and vimentin in the hAMSCs group were notably lower

while that of E-cadherin was higher than the model group (all

0.05).

In vitro

experiment: Compared with the control group

the expression of IκB-α was decreased but that of CK-7 was increased in the induction group; The expression levels of CK-7 and CK-19 were increased significantly in the inhibitor group than the induction group (both

0.05).

Conclusion

hAMSCs transplantation have therapeutic effect on uterine adhesions

which may be due to its promoting the transformation of hAMSCs into endometrial epithelial cells by inhibition of IκB-α/NF-κB pathway activation.

关键词

Keywords

references

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