Role of Dexras1-mediated inflammatory response in white matter damage after subarachnoid hemorrhage in rats

Yuanjun XIN; Hao HUANG; Yidan LIANG; Wang QIN; Xintong WU; Xuefeng LUO; Fuming LIANG; Zhaohui HE

doi:10.16016/j.1000-5404.202002207

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Role of Dexras1-mediated inflammatory response in white matter damage after subarachnoid hemorrhage in rats

Basic Medicine | 更新时间：2026-01-09

- Role of Dexras1-mediated inflammatory response in white matter damage after subarachnoid hemorrhage in rats
- Journal of Third Military Medical University Vol. 42, Issue 13, Pages: 1315-1322(2020)
- 作者机构：
  
  400016 重庆, 重庆医科大学附属第一医院神经外科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81870927);the General Program of Natural Science Foundation of Chongqing(CSTC2019jcyj-msxmX0239)
- DOI：10.16016/j.1000-5404.202002207
  CLC： R363.21;R741.02;R743.35
- Received：27 February 2020，
  
  Revised：2020-03-13，
  
  Published：15 July 2020
- 稿件说明：
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Yuanjun XIN, Hao HUANG, Yidan LIANG, et al. Role of Dexras1-mediated inflammatory response in white matter damage after subarachnoid hemorrhage in rats[J]. Journal of Third Military Medical University, 2020, 42(13): 1315-1322.
DOI：

Yuanjun XIN, Hao HUANG, Yidan LIANG, et al. Role of Dexras1-mediated inflammatory response in white matter damage after subarachnoid hemorrhage in rats[J]. Journal of Third Military Medical University, 2020, 42(13): 1315-1322. DOI： 10.16016/j.1000-5404.202002207.

摘要

目的

探索Dexras1在蛛网膜下腔出血(subarachnoid hemorrhage

SAH)中促进炎症和参与白质损伤(white matter damage

WMD)的机制。

方法

将90只成年健康雄性SD大鼠作为研究对象

采用随机抽签的形式将其分为假手术sham组、SAH组、SAH时间点组、SAH+LV-Scramble组、SAH+LV-Dexras1

组和SAH+LV-Dexras1

组

每组12只

利用血管内穿刺法构建大鼠在体蛛网膜下腔出血模型

免疫荧光及神经脊髓固蓝染色分别观察各组皮层髓鞘变化

神经行为学评分检测神经功能变化情况；Western blot检测各组Dexras1、TNF-α、IL-1β及MBP的表达。

结果

与sham组比较

SAH后大鼠脑组织中Dexras1、TNF-α、IL-1β明显升高(

0.01)

MBP明显减少(

0.01)

出现明显的神经功能缺损、脑水肿、髓鞘损伤及炎症细胞浸润；与SAH+LV-Scramble组比较

SAH+LV-Dexras1

组大鼠脑组织中Dexras1、TNF-α、IL-1β继续升高(

0.01)

MBP进一步降低(

0.05)

神经功能缺损、脑水肿、髓鞘损伤明显加重

炎症细胞浸润也显著增多；而SAH+LV-Dexras1

组大鼠脑组织中Dexras1、TNF-α、IL-1β明显降低(

0.01)

MBP升高(

0.05)

神经功能缺损、脑水肿、髓鞘损伤明显改善

炎症细胞浸润也显著减少。

结论

Dexras1在蛛网膜下腔出血中具有促进炎症反应

从而参与蛛网膜下腔出血后白质损伤的作用。

Abstract

Objective

To explore the mechanisms by which Dexras1 promotes inflammation and participates in white matter damage (WMD) after subarachnoid hemorrhage (SAH).

Methods

Ninety healthy adult male SD rats were randomly divided into control group (sham group)

SAH group

SAH time ponits group

SAH+LV-Scramble group

SAH+LV-Dexras1+ group

and SAH+LV-Dexras1

group

with 12 rats in each group. Intravascular puncture was used to establish a rat model of intracranial hemorrhage under subarachnoid. Immunofluorescence assay and Luxol fast blue staining were used to detect myelin changes in each group; Neurobehavioral scores were used to evaluate the changes in neurological function; Western blot analysis was used to detect the expression of Dexras1

TNF-α

IL-1β and myelin basic protein (MBP) in each group.

Results

Compared with the sham group

Dexras1

TNF-α

and IL-1β were significantly increased in the brain tissue (

0.01)

MBP was significantly reduced (

0.01)

and obvious neurological deficits

cerebral edema

myelin sheath injury and inflammatory cell infiltration were observed in the SAH group. Compared with the SAH+LV-Scramble group

Dexras1

TNF-α

and IL-1β in the brain tissue of the SAH+LV-Dexras1+ group continued to increase (

0.01)

MBP was further reduced (

0.05)

and neurological deficits

cerebral edema

and myelin sheath damage were significantly aggravated

and inflammatory cell infiltration was also significantly increased in the group. However

Dexras1

TNF-α

and IL-1β in the brain tissue were significantly reduced (

0.01)

MBP was increased (

0.05)

and neurological deficits

cerebral edema and myelin sheath damage were obviously improved

inflammatory cell infiltration was significantly reduced in the SAH+LV-Dexras1

group.

Conclusion

Dexras1 can promote the inflammatory response

and thereby participate in white matter damage after SAH.

关键词

Keywords

references

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