Changes of PLCγ1/IP3R/CaMKⅡ protein expression and dendritic spine density in hippocampus of VPA-induced autistic young mice

Yu ZHAO; Yan WANG; Wenxia ZHENG; Yuling HU; Xiaoqing ANG; Di CHEN; Yingbo LI; Rong YANG; Shali WANG

doi:10.16016/j.1000-5404.202002189

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Changes of PLCγ1/IP3R/CaMKⅡ protein expression and dendritic spine density in hippocampus of VPA-induced autistic young mice

Neurosciences | 更新时间：2026-01-09

- Changes of PLCγ1/IP3R/CaMKⅡ protein expression and dendritic spine density in hippocampus of VPA-induced autistic young mice
- Journal of Third Military Medical University Vol. 42, Issue 13, Pages: 1266-1272(2020)
- 作者机构：
  
  400016 重庆, 重庆医科大学基础医学院神经科学研究中心
- 作者简介：
- 基金信息：
  
  the Project of Basic Research and Frontier Exploration of Chongqing Municipal Science and Technology Commission(CSTC2018jcyjAX0646)
- DOI：10.16016/j.1000-5404.202002189
  CLC： R338.26;R363.21;R749.94
- Received：26 February 2020，
  
  Revised：2020-03-28，
  
  Published：15 July 2020
- 稿件说明：
移动端阅览
Yu ZHAO, Yan WANG, Wenxia ZHENG, et al. Changes of PLCγ1/IP3R/CaMKⅡ protein expression and dendritic spine density in hippocampus of VPA-induced autistic young mice[J]. Journal of Third Military Medical University, 2020, 42(13): 1266-1272.
DOI：

Yu ZHAO, Yan WANG, Wenxia ZHENG, et al. Changes of PLCγ1/IP3R/CaMKⅡ protein expression and dendritic spine density in hippocampus of VPA-induced autistic young mice[J]. Journal of Third Military Medical University, 2020, 42(13): 1266-1272. DOI： 10.16016/j.1000-5404.202002189.

摘要

目的

探讨丙戊酸钠(VPA)诱导的自闭症模型小鼠大脑海马区磷脂酶C-γ1(PLCγ1)/肌醇三磷酸受体(IP3R)/钙调蛋白依赖性激酶Ⅱ(CaMKⅡ)表达的改变和树突棘密度的异常。

方法

C57孕小鼠于孕10、12 d分别腹腔注射丙戊酸钠300 mg/kg或生理盐水，其仔鼠分别为自闭症谱系障碍模型鼠(VPA组)或正常对照鼠(CON组)。Western blot检测两组仔鼠大脑海马区PLCγ1、IP3R、CaMKⅡ和突触后致密物-95(PSD95)的表达水平。高尔基染色法检测2组仔鼠大脑海马区树突棘的变化。

结果

Western blot检测结果显示：与正常对照组相比，VPA组小鼠大脑海马区PLCγ1，IP3R，CaMKⅡ和PSD95的表达均下降(

0.01)。高尔基染色结果显示：与正常对照组相比，VPA组小鼠大脑海马区树突棘密度减少(

0.01)。

结论

VPA诱导的自闭症模型小鼠大脑海马区的PLCγ1/IP3R/CaMKⅡ表达下降，同时伴随着海马CA1区锥体神经元树突棘密度降低，PSD95的表达减少。

Abstract

Objective

To investigate expression changes of phospholipase C-γ1 (PLCγ1)/inositol triphosphate receptor (IP3R)/calmodulin-dependent kinase Ⅱ (CaMKⅡ) and density of dendritic spine in the hippocampus of autism model mice induced by sodium valproate (VPA).

Methods

Pregnant C57 mouse was given intraperitoneal injection of 300 mg/kg VPA or same volume of normal saline at the 10th and 12th days of gestation. The offspring exposed to VPA or saline during gestation were identified as autism spectrum disorder mice (VPA group) or normal control mice (CON group). Western blotting was used to detect the expression levels of PLCγ1

IP3R

CaMKⅡ and post-synaptic dense-95 (PSD95) in the hippocampus of the 2 groups. Golgi staining was employed to observe the changes in dendritic spines in the hippocampus of the offspring mice.

Results

Western blotting showed that the expression levels of PLCγ1

IP3R

CaMKⅡ and PSD95 were decreased in the VPA group than the normal control group (

0.01). Golgi staining indicated that compared with the normal control group

the density of dendritic spines in the hippocampus of VPA mice was reduced (

0.01).

Conclusion

In the hippocampus of VPA induced autism mice

the expression of PLCγ1/IP3R/CaMKⅡ and PSD95 and the dendritic spine density of pyramidal neurons in hippocampal CA1 are all decreased compared with the normal control mice.

关键词

Keywords

references

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