Killing effect of ivermectin on migratory-stage schistosomula of
Schistosoma japonicum - Journal of Third Military Medical University Vol. 42, Issue 13, Pages: 1286-1291(2020)
- 作者机构：
1.410013 长沙，中南大学湘雅医学院寄生虫系与细胞生物学中心
2.414000 湖南 岳阳，岳阳职业技术学院
3.410078 长沙，中南大学高等研究中心细胞生物学研究室
- 作者简介：
- 基金信息：the Project of Scientific Research of Hunan Provincial Department of Education(18C1764)
DOI：10.16016/j.1000-5404.202002146
CLC： R-332;R383.24;R978.15Received：21 February 2020，
Revised：2020-04-07，
Published：15 July 2020
- 稿件说明：
移动端阅览
Dong LAN, Qingren ZENG, Xuan MA, et al. Killing effect of ivermectin on migratory-stage schistosomula of
Schistosoma japonicum [J]. Journal of Third Military Medical University, 2020, 42(13): 1286-1291.Dong LAN, Qingren ZENG, Xuan MA, et al. Killing effect of ivermectin on migratory-stage schistosomula of
Schistosoma japonicum [J]. Journal of Third Military Medical University, 2020, 42(13): 1286-1291. DOI： 10.16016/j.1000-5404.202002146.
摘要
目的
2
从抗其他病原感染的大环内酯类药物中筛选一种可杀日本血吸虫(
Schistosoma japonicum
S.j
)移行期童虫的制剂。
方法
2
选取克拉霉素(ClM)、阿奇霉素(AzM)和伊维菌素(IVM)在体外实验中设计3个不同剂量水平对
S.j
脱尾童虫作杀伤试验观察，以行为改变和染色差异作为判别虫体死与活的标志，以各药物组虫体死亡率大小来评价杀伤效果。取体外杀虫高效的IVM用于
S.j
感染小鼠后体内1~4 d虫体作治疗效果观察。
结果
2
在体外IVM 20 μg/mL浓度条件下对
S.j
尾蚴脱尾童虫可致100%的杀伤率，且显著高于吡喹酮(PZQ)和青蒿琥酯(ARS)的杀伤效果(
P
<
0.05)。IVM在0.4 mg/kg×2次的剂量对
S.j
感染小鼠后1~2 d的童虫杀死率为53.37%，与感染对照组比差异有统计学意义(
P
<
0.01)；对3~4 d的童虫杀死率为39.98%，与感染对照组相比差异有统计学意义(
P
<
0.05)。用IVM与PZQ或ARS联合治疗，未出现增效或协同作用。
结论
2
大环内酯类药物中伊维菌素对体内外日本血吸虫移行期童虫有很好的杀伤效果。
Abstract
Objective
2
To screen an effective drug which can kill migratory-stage schistosomula of
Schistosoma japonicum
(
S. j
) from macrocyclic drugs treating other pathogenic infections.
Methods
2
Firstly
3 doses of clarithromycin (ClM)
azithromycin (AzM) and ivermectin (IVM) were used to kill the migratory-stage schistosomula
in vitro
. The behavior changes and difference in methylene blue staining were used as markers to distinguish the death or life of the worms. The mortality of each group was employed to evaluate the killing effects of different drug groups. IVM at the dose of highly killing effectiveness was used to treat the mice in 1 to 4 d after
S.j
infection in order to observe the therapeutic effect.
Results
2
IVM at a concentration of 20 μg/mL showed 100% killing effect against early-stage schistosomula
in vitro
which was significantly higher than that of praziquantel (PZQ) and artesunate (ARS) (
P
<
0.05). When IVM was used to treat the mice at the dose of 0.4 mg/kg×2
the killing rate of
S.j
was 53.37% in 1~2 d (
P
<
0.01) and 39.98% in 3~4 d (
P
<
0.05) after infection
with statistical difference when compared with the control group. No synergistic or additive effect was observed when IVM was combined with PZQ or ARS.
Conclusion
2
Among macrocyclic drugs
IVM exerts good killing effect on migratory-stage schistosomula of
S.j in vivo
and
in vitro
.
关键词
Keywords
references
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