Heregulin-β1 promotes proliferation, migration and invasion of breast cancer cells by inducing stearoyl-CoA desaturase-1

Jingruo CHEN; Xuejiao MA; Sinan LYU; Qi'er AN; Yuhua ZHAO

doi:10.16016/j.1000-5404.202001179

您当前的位置：

首页 >

文章列表页 >

Heregulin-β1 promotes proliferation, migration and invasion of breast cancer cells by inducing stearoyl-CoA desaturase-1

更新时间：2026-01-09

- Heregulin-β1 promotes proliferation, migration and invasion of breast cancer cells by inducing stearoyl-CoA desaturase-1
- Journal of Third Military Medical University Vol. 42, Issue 11, Pages: 1091-1100(2020)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.202001179
  CLC：
- Received：20 January 2020，
  
  Published：15 June 2020
- 稿件说明：
移动端阅览
Jingruo CHEN, Xuejiao MA, Sinan LYU, et al. Heregulin-β1 promotes proliferation, migration and invasion of breast cancer cells by inducing stearoyl-CoA desaturase-1[J]. Journal of Third Military Medical University, 2020, 42(11): 1091-1100.
DOI：

Jingruo CHEN, Xuejiao MA, Sinan LYU, et al. Heregulin-β1 promotes proliferation, migration and invasion of breast cancer cells by inducing stearoyl-CoA desaturase-1[J]. Journal of Third Military Medical University, 2020, 42(11): 1091-1100. DOI： 10.16016/j.1000-5404.202001179.

摘要

Abstract

Objective

To investigate the heregulin-β1 (HRG-β1)-induced expression of stearoyl-CoA desaturase-1 (SCD1) and the effect of its activity of SCD1 on the proliferation

migration and invasion of breast cancer cells MCF7 and SK-BR-3.

Methods

MCF7 and SK-BR-3 cells were treated with control (PBS) or HRG-β1 at different concentrations and for different times. Then RT-qPCR and Western blotting were used to detect the mRNA and protein expression levels of SCD1. The wound healing assay and Transwell assay were employed to measure the migration and invasion of MCF7 and SK-BR-3 cells treated with PBS

HRG-β1

A939572 and oleic acid (OA).

Results

HRG-β1 increased both SCD1 mRNA and protein levels in MCF7 and SK-BR-3 cells at a time-dose dependent manner. Compared with the control group

HRG-β1 promoted cell proliferation in MCF7 and SK-BR-3 cells (

0.01)

while proliferation rate was decreased after A939572 treatment (

0.01). Compared with the control group

HRG-β1 significantly increased the wound healing rates and invaded cell numbers of MCF7 and SK-BR-3 cells (

0.01). The wound healing rates and invaded cell numbers of both cell lines were significantly decreased after HRG-β1+A939572 treatment (

0.01). The addition of OA rescued the migration and invasion of both cell lines which were weakened by A939572 (

0.01).

Conclusions

HRG-β1 can up-regulate the expression of SCD1

and the protein partially mediates the effect of HRG-β1 in promoting the proliferation

migration and invasion in MCF7 and SK-BR-3 cells.

关键词

Keywords

references

BREULEUX M Role of heregulin in human cancer Cell Mol Life Sci 2007 64 18 2358 2377 10.1007/s00018-007-7120-0 BREULEUX M. Role of heregulin in human cancer[J]. Cell Mol Life Sci, 2007, 64(18): 2358-2377. DOI:10.1007/s00018-007-7120-0.

FALLS D L Neuregulins: functions, forms, and signaling strategies Exp Cell Res 2003 284 1 14 30 10.1016/s0014-4827(02)00102-7 FALLS D L. Neuregulins: functions, forms, and signaling strategies[J]. Exp Cell Res, 2003, 284(1): 14-30. DOI:10.1016/s0014-4827(02)00102-7.

NAGASAKA M OU S I Neuregulin 1 Fusion-positive NSCLC J Thorac Oncol 2019 14 8 1354 1359 10.1016/j.jtho.2019.05.015 NAGASAKA M, OU S I. Neuregulin 1 Fusion-positive NSCLC[J]. J Thorac Oncol, 2019, 14(8): 1354-1359. DOI:10.1016/j.jtho.2019.05.015.

DE ALAVA E OCAÑA A ABAD M Neuregulin expression modulates clinical response to trastuzumab in patients with metastatic breast cancer J Clin Oncol 2007 25 19 2656 2663 10.1200/JCO.2006.08.6850 10.1200/JCO.2006.08.6850 DE ALAVA E, OCAÑA A, ABAD M, et al .Neuregulin expression modulates clinical response to trastuzumab in patients with metastatic breast cancer[J ] . J Clin Oncol, 2007, 25(19): 2656-2663. DOI:10.1200/JCO.2006.08.6850.

YAMASHITA N SAITO N ZHAO S Heregulin-induced cell migration is promoted by aryl hydrocarbon receptor in HER2-overexpressing breast cancer cells Exp Cell Res 2018 366 1 34 40 10.1016/j.yexcr.2018.02.033 YAMASHITA N, SAITO N, ZHAO S, et al .Heregulin-induced cell migration is promoted by aryl hydrocarbon receptor in HER2-overexpressing breast cancer cells[J ] . Exp Cell Res, 2018, 366(1): 34-40. DOI:10.1016/j.yexcr.2018.02.033.

ZUO B L YAN B ZHENG G X Targeting and suppression of HER3-positive breast cancer by T lymphocytes expressing a heregulin chimeric antigen receptor Cancer Immunol Immunother 2018 67 3 393 401 10.1007/s00262-017-2089-5 ZUO B L, YAN B, ZHENG G X, et al .Targeting and suppression of HER3-positive breast cancer by T lymphocytes expressing a heregulin chimeric antigen receptor[J ] . Cancer Immunol Immunother, 2018, 67(3): 393-401. DOI:10.1007/s00262-017-2089-5.

MENENDEZ J A LUPU R Fatty acid synthase and the lipogenic phenotype in cancer pathogenesis Nat Rev Cancer 2007 7 10 763 777 10.1038/nrc2222 MENENDEZ J A, LUPU R. Fatty acid synthase and the lipogenic phenotype in cancer pathogenesis[J]. Nat Rev Cancer, 2007, 7(10): 763-777. DOI:10.1038/nrc2222.

ALJOHANI A M SYED D N NTAMBI J M Insights into stearoyl-CoA desaturase-1 regulation of systemic metabolism Trends Endocrinol Metab 2017 28 12 831 842 10.1016/j.tem.2017.10.003 ALJOHANI A M, SYED D N, NTAMBI J M. Insights into stearoyl-CoA desaturase-1 regulation of systemic metabolism[J]. Trends Endocrinol Metab, 2017, 28(12): 831-842. DOI:10.1016/j.tem.2017.10.003.

TRACZ-GASZEWSKA Z DOBRZYN P Stearoyl-CoA desaturase 1 as a therapeutic target for the treatment of cancer Cancers (Basel) 2019 11 7 E948 10.3390/cancers11070948 TRACZ-GASZEWSKA Z, DOBRZYN P. Stearoyl-CoA desaturase 1 as a therapeutic target for the treatment of cancer[J]. Cancers (Basel), 2019, 11(7): E948. DOI:10.3390/cancers11070948.

MERINO SALVADOR M GÓMEZ DE CEDRÓN M MORENO RUBIO J Lipid metabolism and lung cancer Crit Rev Oncol 2017 112 31 40 10.1016/j.critrevonc.2017.02.001 MERINO SALVADOR M, GÓMEZ DE CEDRÓN M, MORENO RUBIO J, et al .Lipid metabolism and lung cancer[J ] . Crit Rev Oncol, 2017, 112: 31-40. DOI:10.1016/j.critrevonc.2017.02.001.

LIU G H FENG S JIA L Lung fibroblasts promote metastatic colonization through upregulation of stearoyl-CoA desaturase 1 in tumor cells Oncogene 2018 37 11 1519 1533 10.1038/s41388-017-0062-6 LIU G H, FENG S, JIA L, et al .Lung fibroblasts promote metastatic colonization through upregulation of stearoyl-CoA desaturase 1 in tumor cells[J ] . Oncogene, 2018, 37(11): 1519-1533. DOI:10.1038/s41388-017-0062-6.

HOLDER A M GONZALEZ-ANGULO A M CHEN H Q High stearoyl-CoA desaturase 1 expression is associated with shorter survival in breast cancer patients Breast Cancer Res Treat 2013 137 1 319 327 10.1007/s10549-012-2354-4 HOLDER A M, GONZALEZ-ANGULO A M, CHEN H Q, et al .High stearoyl-CoA desaturase 1 expression is associated with shorter survival in breast cancer patients[J ] . Breast Cancer Res Treat, 2013, 137(1): 319-327. DOI:10.1007/s10549-012-2354-4.

RAN H ZHU Y M DENG R Y Stearoyl-CoA desaturase-1 promotes colorectal cancer metastasis in response to glucose by suppressing PTEN J Exp Clin Cancer Res 2018 37 1 54 10.1186/s13046-018-0711-9 RAN H, ZHU Y M, DENG R Y, et al .Stearoyl-CoA desaturase-1 promotes colorectal cancer metastasis in response to glucose by suppressing PTEN[J ] . J Exp Clin Cancer Res, 2018, 37(1): 54. DOI:10.1186/s13046-018-0711-9.

李卫华杨佳欣硬脂酰辅酶A去饱和酶1与恶性肿瘤的研究进展中国肿瘤临床 2014 41 17 1131 1134 10.3969/j.issn.1000-8179.20140483.LIWH, 李卫华, 杨佳欣.硬脂酰辅酶A去饱和酶1与恶性肿瘤的研究进展[J].中国肿瘤临床, 2014, 41(17): 1131-1134. DOI:10.3969/j.issn.1000-8179.20140483.LIW H,

LI W H YANG J X Development in the study of the correlation between stearoyl-coenzyme A desaturase 1 and cancer progression Chin J Clin Oncol 2014 41 17 1131 1134 10.3969/j.issn.1000-8179.20140483 YANG J X. Development in the study of the correlation between stearoyl-coenzyme A desaturase 1 and cancer progression[J]. Chin J Clin Oncol, 2014, 41(17): 1131-1134. DOI:10.3969/j.issn.1000-8179.20140483.

SHE K L FANG S H DU W SCD1 is required for EGFR-targeting cancer therapy of lung cancer via re-activation of EGFR/PI3K/AKT signals Cancer Cell Int 2019 19 103 10.1186/s12935-019-0809-y SHE K L, FANG S H, DU W, et al .SCD1 is required for EGFR-targeting cancer therapy of lung cancer via re-activation of EGFR/PI3K/AKT signals[J ] . Cancer Cell Int, 2019, 19: 103. DOI:10.1186/s12935-019-0809-y.

ZHAO G CARDENAS H MATEI D Ovarian cancer-why lipids matter Cancers (Basel) 2019 11 12 1870 10.3390/cancers11121870 ZHAO G, CARDENAS H, MATEI D. Ovarian cancer-why lipids matter[J]. Cancers (Basel), 2019, 11(12): 1870. DOI: 10.3390/cancers11121870.

MAUVOISIN D CHARFI C LOUNIS A M Decreasing stearoyl-CoA desaturase-1 expression inhibits β-catenin signaling in breast cancer cells Cancer Sci 2013 104 1 36 42 10.1111/cas.12032 MAUVOISIN D, CHARFI C, LOUNIS A M, et al .Decreasing stearoyl-CoA desaturase-1 expression inhibits β-catenin signaling in breast cancer cells[J ] . Cancer Sci, 2013, 104(1): 36-42. DOI:10.1111/cas.12032.

KATARIA H ALIZADEH A KARIMI-ABDOLREZAEE S Neuregulin-1/ErbB network: an emerging modulator of nervous system injury and repair Prog Neurobiol 2019 180 101643 10.1016/j.pneurobio.2019.101643 KATARIA H, ALIZADEH A, KARIMI-ABDOLREZAEE S. Neuregulin-1/ErbB network: an emerging modulator of nervous system injury and repair[J]. Prog Neurobiol, 2019, 180: 101643. DOI:10.1016/j.pneurobio.2019.101643.

HIJAZI M M THOMPSON E W TANG C Heregulin regulates the actin cytoskeleton and promotes invasive properties in breast cancer cell lines Int J Oncol 2000 17 4 629 641 10.3892/ijo.17.4.629 HIJAZI M M, THOMPSON E W, TANG C, et al .Heregulin regulates the actin cytoskeleton and promotes invasive properties in breast cancer cell lines[J ] . Int J Oncol, 2000, 17(4): 629-641. DOI:10.3892/ijo.17.4.629.

HASKINS J W NGUYEN D X STERN D F Neuregulin 1-activated ERBB4 interacts with YAP to induce Hippo pathway target genes and promote cell migration Sci Signal 2014 7 355 ra116 10.1126/scisignal.2005770 HASKINS J W, NGUYEN D X, STERN D F. Neuregulin 1-activated ERBB4 interacts with YAP to induce Hippo pathway target genes and promote cell migration[J]. Sci Signal, 2014, 7(355): ra116. DOI:10.1126/scisignal.2005770.

RUAN S Q WANG S W WANG Z H Regulation of HRG-β1-induced proliferation, migration and invasion of MCF-7 cells by upregulation of GPR30 expression Mol Med Rep 2012 6 1 131 138 10.3892/mmr.2012.874 RUAN S Q, WANG S W, WANG Z H, et al .Regulation of HRG-β1-induced proliferation, migration and invasion of MCF-7 cells by upregulation of GPR30 expression[J ] . Mol Med Rep, 2012, 6(1): 131-138. DOI:10.3892/mmr.2012.874.

ASRANI K KERI R A GALISTEO R The HER2- and heregulin β1 (HRG)-inducible TNFR superfamily member Fn14 promotes HRG-driven breast cancer cell migration, invasion, and MMP9 expression Mol Cancer Res 2013 11 4 393 404 10.1158/1541-7786.MCR-12-0542 ASRANI K, KERI R A, GALISTEO R, et al .The HER2- and heregulin β1 (HRG)-inducible TNFR superfamily member Fn14 promotes HRG-driven breast cancer cell migration, invasion, and MMP9 expression[J ] . Mol Cancer Res, 2013, 11(4): 393-404. DOI:10.1158/1541-7786.MCR-12-0542.

ZHANG J Q SONG F ZHAO X J EGFR modulates monounsaturated fatty acid synthesis through phosphorylation of SCD1 in lung cancer Mol Cancer 2017 16 1 127 10.1186/s12943-017-0704-x ZHANG J Q, SONG F, ZHAO X J, et al .EGFR modulates monounsaturated fatty acid synthesis through phosphorylation of SCD1 in lung cancer[J ] . Mol Cancer, 2017, 16(1): 127. DOI:10.1186/s12943-017-0704-x.

CHENG L S ZHA Z LANG B Heregulin-beta1 promotes metastasis of breast cancer cell line SKBR3 through upregulation of Snail and induction of epithelial-mesenchymal transition Cancer Lett 2009 280 1 50 60 10.1016/j.canlet.2009.02.007 CHENG L S, ZHA Z, LANG B, et al .Heregulin-beta1 promotes metastasis of breast cancer cell line SKBR3 through upregulation of Snail and induction of epithelial-mesenchymal transition[J ] . Cancer Lett, 2009, 280(1): 50-60. DOI:10.1016/j.canlet.2009.02.007.

PORSTMANN T SANTOS C R GRIFFITHS B SREBP activity is regulated by mTORC1 and contributes to Akt-dependent cell growth Cell Metab 2008 8 3 224 236 10.1016/j.cmet.2008.07.007 PORSTMANN T, SANTOS C R, GRIFFITHS B, et al .SREBP activity is regulated by mTORC1 and contributes to Akt-dependent cell growth[J ] . Cell Metab, 2008, 8(3): 224-236. DOI:10.1016/j.cmet.2008.07.007.

GRIFFITHS B LEWIS C A BENSAAD K Sterol regulatory element binding protein-dependent regulation of lipid synthesis supports cell survival and tumor growth Cancer Metab 2013 1 1 3 10.1186/2049-3002-1-3 GRIFFITHS B, LEWIS C A, BENSAAD K, et al .Sterol regulatory element binding protein-dependent regulation of lipid synthesis supports cell survival and tumor growth[J ] . Cancer Metab, 2013, 1(1): 3. DOI:10.1186/2049-3002-1-3.

蒋素芳屠凯岭周兰 Heregulin-β1诱导的糖酵解对乳腺癌细胞MCF7迁移的影响四川大学学报(医学版) 2016 47 4 463 467 10.13464/j.scuxbyxb.2016.04.003 蒋素芳, 屠凯岭, 周兰, 等. Heregulin-β1诱导的糖酵解对乳腺癌细胞MCF7迁移的影响[J].四川大学学报(医学版), 2016, 47(4): 463-467. DOI:10.13464/j.scuxbyxb.2016.04.003.

JIANG S F TU K L ZHOU L Heregulin-β1-induced glycolysis promotes migration of breast cancer cell line MCF7 J Sichuan Univ Med Sci Ed 2016 47 4 463 467 10.13464/j.scuxbyxb.2016.04.003 JIANG S F, TU K L, ZHOU L, et al .Heregulin-β1-induced glycolysis promotes migration of breast cancer cell line MCF7[J ] . J Sichuan Univ Med Sci Ed, 2016, 47(4): 463-467. DOI:10.13464/j.scuxbyxb.2016.04.003.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation, migration and invasion

Mechanism of CPNE3 regulating proliferation, migration and invasion of non-small cell lung cancer cells

Effect of RNA sensor IFIH1 on proliferation, migration and invasion of HPV positive cervical cancer cells

Effect of transmembrane protein 196 on migration and invasion of breast cancer

Dihydromyricetin inhibits migration and invasion of human breast cancer cells by suppressing mTOR signaling pathway

Related Author

HAO Xiaohui

CHEN Min

WU Nan

DING Yunshan

ZHU Lifan

DAI Haitao

Xin CAI

Zeyi LIU

Related Institution

School of Medicine， Shihezi University

Department of Stomatology， the First Affiliated Hospital of Shihezi University

Department of Pulmonary and Critical Care Medicine, Institute of Respiratory Diseases, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Provmce

Institute of Life Sciences, Chongqing Medical University

Department of Breast and Thyroid Surgery, Army Medical Center of PLA

⁰