Lethal triad and multi-organ dysfunction in rats following cold seawater bath and hemorrhagic shock

Yanli LIU; Kunlun TIAN; Yu ZHU; Yue WU; Liangming LIU; Lei LIU

doi:10.16016/j.1000-5404.201912181

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Lethal triad and multi-organ dysfunction in rats following cold seawater bath and hemorrhagic shock

更新时间：2026-01-09

- Lethal triad and multi-organ dysfunction in rats following cold seawater bath and hemorrhagic shock
- Journal of Third Military Medical University Vol. 42, Issue 8, Pages: 752-757(2020)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201912181
  CLC：
- Received：19 December 2019，
  
  Published：30 April 2020
- 稿件说明：
移动端阅览
Yanli LIU, Kunlun TIAN, Yu ZHU, et al. Lethal triad and multi-organ dysfunction in rats following cold seawater bath and hemorrhagic shock[J]. Journal of Third Military Medical University, 2020, 42(8): 752-757.
DOI：

Yanli LIU, Kunlun TIAN, Yu ZHU, et al. Lethal triad and multi-organ dysfunction in rats following cold seawater bath and hemorrhagic shock[J]. Journal of Third Military Medical University, 2020, 42(8): 752-757. DOI： 10.16016/j.1000-5404.201912181.

摘要

Abstract

Objective

To investigate the effect of hypothermia induced by cold seawater bath and hemorrhagic shock on lethal triad

vital organ dysfunction and survival time in rats.

Methods

Male SD rats were subjected to cold (15 ℃) seawater bath for 2 h or blood extraction of 40% of the total blood volume from the right carotid artery

or both treatments. Blood gas

blood coagulation function and vital organ functions were detected at 4 h after successful modeling

and the survival of the rats at 72 h was observed.

Results

A 40% blood loss caused the core body temperature and pH value to fluctuate mildly within the normal range and partial impairment the organ functions of the rats. Cold seawater bath for 2 h resulted in rapid onset of hypothermia

coagulation dysfunction

organ function damages and a high mortality rate of the rats. Cold seawater bath further exacerbated the detrimental effects of hemorrhagic shock to cause the core body temperature to drop to 33 ℃

which failed to recover the normal temperature even after the bath. Blood gas analysis showed that cold seawater bath and hemorrhagic shock combined lowered the pH value to 7.17 and reduced the other acid and base indexes

indicating the onset of metabolic acidosis; the combined treatment also induced coagulation dysfunctions shown by obviously prolonged prothrombin time(PT) and activated partial thrombinplastin time (APTT)

significantly decreased fibrinogen(FIB)

and significantly increased international normalized ratio(INR). In addition

cold seawater bath significantly increased the blood parameters for the heart

liver and kidney functions in rats with hemorrhagic shock

suggesting severe functional damage of multiple organs. The survival rate was reduced to 3/16 and the survival time was shortened to 21.7 h in the rats exposed to the combined treatment.

Conclusion

Cold seawater bath causes a high incidence of fatal triad

induces functional impairment of the vital organs and increases the mortality of the rats with hemorrhagic shock.

关键词

Keywords

references

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