Microecology of tongue coating flora in patients with rheumatoid arthritis

Chaofei MAO; Hui SUN; Qinghua ZOU; Ying SHI; Li YANG; Ping WAN; Yongfei FANG

doi:10.16016/j.1000-5404.201912166

您当前的位置：

首页 >

文章列表页 >

Microecology of tongue coating flora in patients with rheumatoid arthritis

Clinical Medicine | 更新时间：2026-01-09

- Microecology of tongue coating flora in patients with rheumatoid arthritis
- Journal of Third Military Medical University Vol. 42, Issue 7, Pages: 708-713(2020)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院中医与风湿免疫科
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81072921)
- DOI：10.16016/j.1000-5404.201912166
  CLC： R241.25;R446.5;R593.22
- Received：08 December 2019，
  
  Revised：2020-02-07，
  
  Published：15 April 2020
- 稿件说明：
移动端阅览
Chaofei MAO, Hui SUN, Qinghua ZOU, et al. Microecology of tongue coating flora in patients with rheumatoid arthritis[J]. Journal of Third Military Medical University, 2020, 42(7): 708-713.
DOI：

Chaofei MAO, Hui SUN, Qinghua ZOU, et al. Microecology of tongue coating flora in patients with rheumatoid arthritis[J]. Journal of Third Military Medical University, 2020, 42(7): 708-713. DOI： 10.16016/j.1000-5404.201912166.

摘要

目的

从舌苔菌群微生态上研究类风湿关节炎患者疾病进展与菌群的关系。

方法

使用口腔咽拭子刮取活动期(OP组)、缓解期(OR组)及健康对照组(OHC)各10例的舌苔，使用口腔拭子基因组DNA快速提取试剂盒提取总DNA，进行16S rRNA扩增并进行Illumina MiSeq高通量测序，对测序结果进行微生态分析。

结果

3组样本在菌群的多样性上差异无统计学意义(

0.05)；3组样本有着各自独有的菌属，其中OP组7个菌

属，OR组4个菌属，OHC组10个菌属为各组独有；3组样本的优势菌群组成相同，但是在菌群构成中差异具有统计学意义，主要在厚壁菌门、放线菌门、Episilonbacteraeota菌门、变形菌门、奈瑟菌属、放线菌属、弯曲杆菌属和卟啉单胞菌属上(

均

0.05)。进行Beta多样性分析，通过PCA分析以及层级聚类分析，OP组和OHC组能够很好地聚集到一起，差异具有统计学意义。

结论

类风湿关节炎患者舌苔菌群与健康人群存在差异，其中主要表现在放线菌属和弯曲杆菌属丰度增加，奈瑟菌属和卟啉单胞菌属丰度减少。

Abstract

Objective

To investigate the relationship between disease progression and microecology of tongue coating flora in patients with rheumatoid arthritis (RA).

Methods

A total of 30 tongue coating flora swabs were collected from 20 RA patients

including 10 in the active stage (OP group) and 10 in the remission stage (OR group)

and 10 healthy individuals (OHC group).The total DNA extracted by rapid oral swab genomic DNA extraction kit was amplified with 16S rRNA and sequenced with high throughput Illumina MiSeq. The results were analyzed by microecology.

Results

There was no statistical difference in the diversity of 3 groups of samples (

0.05). All the 3 groups of samples had their own unique genera

including 7 genera in OP group

4 genera in OR group

and 10 genera in OHC group; The samples from 3 groups had the same composition of dominant flora

but obvious difference was seen in the composition of flora

mainly in

Firmicutes

Actinobacteria

Episilonbacteraeota

Proteobacteria

Neisseria

Actinomycetes

Campylobacter

and

Porphyromonas

(all

0.05). Through PCA analysis and hierarchical cluster analysis

OP group and OHC group can gather together well with significant differences.

Conclusion

There are differences between the flora of tongue coating in RA patients and healthy individuals. The abundances of

Actinomycetes

and

Campylobacter

are increased

while those of

Neisseria

and

Porphyromonas

are decreased.

关键词

Keywords

references

YU C, LI M T, DUAN X W, et al . Chinese registry of rheumatoid arthritis (CREDIT): I. Introduction and prevalence of remission in Chinese patients with rheumatoid arthritis[J ] . Clin Exp Rheumatol, 2018, 36(5): 836-840.

HOLERS V M, DEMORUELLE M K, KUHN K A, et al . Rheumatoid arthritis and the mucosal origins hypothesis: protection turns to destruction[J ] . Nat Rev Rheumatol, 2018, 14(9): 542-557. DOI: 10.1038/s41584-018-0070-0.

DEANEK D, DEMORUELLE M K, KELMENSON L B, et al .Genetic and environmental risk factors for rheumatoid arthritis[J ] .Best Pract Clinic Rheumatol, 2017, 31(1): 3-18. DOI: 10.1016/j.berh.2017.08.003.

WANG Y, SHENG H F, HE Y, et al . Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of illumina tags[J ] . Appl Environ Microbiol, 2012, 78(23): 8264-8271. DOI: 10.1128/AEM.01821-12.

DOMINGUEZ-BELLO M G, COSTELLO E K, CONTRERAS M, et al . Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns[J ] . Proc Natl Acad Sci USA, 2010, 107(26): 11971-11975. DOI: 10.1073/pnas.1002601107.

DISSICK A, REDMAN R S, JONES M, et al . Association of periodontitis with rheumatoid arthritis: a pilot study[J ] . J Periodontol, 2010, 81(2): 223-230. DOI: 10.1902/jop.2009.090309.

HENDLER A, MULLI T K, HUGHES F J, et al . Involvement of autoimmunity in the pathogenesis of aggressive periodontitis[J ] . J Dent Res, 2010, 89(12): 1389-1394. DOI: 10.1177/0022034510381903.

WEERKAMP A H, UYEN H M, BUSSCHER H J. Effect of Zeta potential and surface energy on bacterial adhesion to uncoated and saliva-coated human enamel and dentin[J]. J Dent Res, 1988, 67(12): 1483-1487. DOI: 10.1177/00220345880670120801.

LUNDBERG K, WEGNER N, YUCEL-LINDBERG T, et al . Periodontitis in RA-the citrullinated enolase connection[J ] . Nat Rev Rheumatol, 2010, 6(12): 727-730. DOI: 10.1038/nrrheum.2010.139.

KONIG M F, ABUSLEME L, REINHOLDT J, et al . Aggregatibacter actinomycetemcomitans-induced hypercitrullination links periodontal infection to autoimmunity in rheumatoid arthritis[J ] . Sci Transl Med, 2016, 8(369): 369ra176. DOI: 10.1126/scitranslmed.aaj1921.

KEMPSELL K E, COX C J, HURLE M, et al . Reverse transcriptase-PCR analysis of bacterial rRNA for detection and characterization of bacterial species in arthritis synovial tissue[J ] . Infect Immun, 2000, 68(10): 6012-6026. DOI: 10.1128/iai.68.10.6012-6026.2000.

陈延强, 谢丽萍.类风湿关节炎湿热痹阻证的舌象与活动性指标关系的研究[J].辽宁中医杂志, 2013, 40(6): 1068-1070. DOI: 10.13192/j.ljtcm.2013.06.18.chenyq.082.

CHEN Y Q, XIE L P. Research on connection between tongue presentations of dampness-heat blockage of rheumatoid arthritis and index of disease activity[J]. Liaoning J Tradit Chin Med, 2013, 40(6): 1068-1070. DOI: 10.13192/j.ljtcm.2013.06.18.chenyq.082.

王志中, 方勇飞, 罗彦, 等. 322例湿热痹阻型及寒湿痹阻型类风湿关节炎中医证型的客观化研究[J].中国中西医结合杂志, 2011, 31(4): 466-470.

WANG Z Z, FANG Y F, LUO Y, et al . The objectivity research on 322 rheumatoid arthritis patients of dampness-heat impeding and cold-dampness impeding Chinese medical syndrome types[J ] . Chin J Integr Tradit West Med, 2011, 31(4): 466-470.

李响.舌苔形成的微生态学机制探讨[J].辽宁中医杂志, 2014, 41(6): 1102-1106. DOI: 10.13192/j.issn.1000-1719.2014.06.011.

LI X. Primary discussion of association of microecology and tongue coating[J]. Liaoning J Tradit Chin Med, 2014, 41(6): 1102-1106. DOI: 10.13192/j.issn.1000-1719.2014.06.011.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Analysis of the core and bridge effects of interpersonal, relaxation and medication efficacy in self-management of rheumatoid arthritis patients

Clinical application of 2017 EULAR for rheumatoid arthritis ultrasound scoring: a feasible study of 62 cases

Relationship between methotrexate dose and clinical efficacy for treatment of rheumatoid arthritis

Dual specificity protein phosphatase 3 and tumor necrosis factor-α as prognostic biomarkers and their regulation by tripterygium glycosides in rheumatoid arthritis

Genomic analysis of vaginal microbiome community in female early rheumatoid arthritis patients

Related Author

DENG Yao

ZHANG Sha

XIANG Xiaorun

WAN Ping

REN Qi

LIU Lei

何丽君

何芸

Related Institution

Department of Traditional Chinese Medicine and Rheumatology and Immunology， First Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Thoracic Surgery， First Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Cardiac Surgery， First Affiliated Hospital， Army Medical University （Third Military Medical University）

Hospital Administration Office， First Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Traditional Chinese Medicine and Rheumatology, First Affiliated Hospital, Army Medical University (Third Military Medical University)

⁰