Screening and identification of synaptic regulatory genes in the inner ear of mice with presbycusis

Xiaoling CHEN; Feng TANG; Lifeng JIA; Hai LI; Jingya LI; Wei YUAN

doi:10.16016/j.1000-5404.201912021

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Screening and identification of synaptic regulatory genes in the inner ear of mice with presbycusis

更新时间：2026-01-09

- Screening and identification of synaptic regulatory genes in the inner ear of mice with presbycusis
- Journal of Third Military Medical University Vol. 42, Issue 8, Pages: 765-771(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81873702);Supported by the General Program of National Natural Science Foundation of China(81470694)
- DOI：10.16016/j.1000-5404.201912021
  CLC：
- Received：02 December 2019，
  
  Published：30 April 2020
- 稿件说明：
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Xiaoling CHEN, Feng TANG, Lifeng JIA, et al. Screening and identification of synaptic regulatory genes in the inner ear of mice with presbycusis[J]. Journal of Third Military Medical University, 2020, 42(8): 765-771.
DOI：

Xiaoling CHEN, Feng TANG, Lifeng JIA, et al. Screening and identification of synaptic regulatory genes in the inner ear of mice with presbycusis[J]. Journal of Third Military Medical University, 2020, 42(8): 765-771. DOI： 10.16016/j.1000-5404.201912021.

摘要

Abstract

Objective

To screen synaptic regulatory genes in the inner ear and explore the mechanism of synaptic damage in presbycusis.

Methods

We examined the mRNA expression of the genes in the basilar membrane of the inner ear of adult (4-week-old) mice and aged (40-week-old) mice using transcriptome sequencing. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of the differentially expressed genes (DEGs) were analyzed to identify synaptic regulatory DEGs

and the results were verified using real-time quantitative PCR and immunofluorescence staining.

Results

We identified a total of 3 267 DEGs

including 1 521 down-regulated genes and 1 746 up-regulated genes (FC≥1.5

0.05). GO function enrichment analysis showed that the down-regulated DEGs mainly participated in the regulation of voltage-gated cation channel activity a

nd synaptic function

and the up-regulated DEGs were mostly responsible for regulation of immune functions. We screened 64 synaptic regulatory genes

among which 6 down-regulated potassium channel genes (Kcnq2

Kcnk3

Kcnmb4

Kcnk9

Kcnh5

and Kcnc2) had the highest enrichment. qRT-PCR verified the differential expression of the 6 genes in the inner ear of mice at different ages; immunofluorescence assay demonstrated that the expression of KCNQ2 and KCNMB4 were significantly decreased in the inner ear of aged mice.

Conclusion

Among the synaptic regulatory genes screened in mice with presbycusis

6 potassium channel genes show significantly decreased expressions in the inner ear of aged mice

suggesting their important involvement in synaptic changes in presbycusis.

关键词

Keywords

references

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