Multimodality imaging and photothermal experiments of nanoparticles by iron (Ⅱ) phthalocyanine-based targeted to breast cancer

Yubei HE; Lan HAO; Qianru LI; Meiying HUANG; Yalan XIONG; Chenxi ZHENG; Zhigang WANG; Gengbiao YUAN

doi:10.16016/j.1000-5404.201911260

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Multimodality imaging and photothermal experiments of nanoparticles by iron (Ⅱ) phthalocyanine-based targeted to breast cancer

更新时间：2026-01-09

- Multimodality imaging and photothermal experiments of nanoparticles by iron (Ⅱ) phthalocyanine-based targeted to breast cancer
- Journal of Third Military Medical University Vol. 42, Issue 8, Pages: 772-782(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Supported by the Basic Research and Frontier Exploration Project of Chongqing Science and Technology Commission(CSTC2018jcyjAX0747);the Key Program of National Natural Science Foundation of China(31630026)
- DOI：10.16016/j.1000-5404.201911260
  CLC：
- Received：30 November 2019，
  
  Published：30 April 2020
- 稿件说明：
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Yubei HE, Lan HAO, Qianru LI, et al. Multimodality imaging and photothermal experiments of nanoparticles by iron (Ⅱ) phthalocyanine-based targeted to breast cancer[J]. Journal of Third Military Medical University, 2020, 42(8): 772-782.
DOI：

Yubei HE, Lan HAO, Qianru LI, et al. Multimodality imaging and photothermal experiments of nanoparticles by iron (Ⅱ) phthalocyanine-based targeted to breast cancer[J]. Journal of Third Military Medical University, 2020, 42(8): 772-782. DOI： 10.16016/j.1000-5404.201911260.

摘要

Abstract

Objective

To prepare a novel nanoparticle carrying iron(Ⅱ) phthalocyanine (FePc) coupled with aptamer AS1411 for targeting human breast cancer MCF-7 cells and evaluate its physicochemical properties

targeting ability to MCF-7 cells

photoacoustic/ultrasonic multimodal imaging capabilities

photothermal performance

photoinduced phase transitions

in vitro

and multimodal imaging performance

in vivo

Methods

FePc-encapsulated non-targeted nanoparticles (PLGA@FePc@PFP) were prepared by a two-step phacoemulsification method

and the targeted nanoparticles (AS1411-PLGA@FePc@PFP NPs) were prepared by combining the aptamer AS1411 modified with NH

and the PLGA@FePc@PFP nanoparticles using the carbodiimide method. The physicochemical properties of AS1411-PLGA@FePc@PFP NPs were detected

and laser confocal microscopy and flow cytometry were used to evaluate the binding between AS1411 and the nanoparticles. CCK-8 assay was used to detect the cytotoxicity of AS1411-PLGA@FePc@PFP; laser confocal microscopy was used to observe the

in vitro

targeting ability of the nanoparticles; photoacoustic and ultrasonic imagers were used to test their imaging performance and targeting ability both

in vitro

and

in vivo

. Near-i

nfrared laser (660 nm) and thermal imagers were used to test the

in vitro

photothermal conversion abilities of the nanoparticles

and the changes of the nanoparticles after laser irradiation were observed under an optical microscope. The temperature change of AS1411-PLGA@FePc@ PFP in tumor-bearing nude mice was detected in response to 660 nm laser irradiation.

Results

AS1411-PLGA@FePc@PFP was successfully prepared

which had an average diameter of 208.03±8.96 nm with a narrow particle size distribution

a mean zeta potential of -10.8±1.14 mV

and a FePc encapsulation rate of (83.96±1.61)%. The results of CCK-8 assay showed that the nanoparticles at varying concentrations had no obvious cytotoxicity. Flow cytometry showed an efficient binding of AS1411 with the nanoparticles had a binding rate of (93.31±2.58)%. Laser confocal analysis showed that there were significantly more nanoparticles around MCF-7 cells in the targeted group than in the non-targeted group. Laser irradiation caused the temperature of the nanoparticles carrying FePc to increase rapidly with time

and the phase change of the nanoparticles was observed under an optical microscope

suggesting good photothermal conversion ability of the nanoparticles. Photoacoustic imaging results showed that compared with nanoparticles without FePc

AS1411-PLGA@FePc@PFP produced strong photoacoustic signals

and the signal intensity showed a positive linear correlation with the nanoparticle concentration. Ultrasound and imaging results showed FePc in AS1411-PLGA@FePc@PFP achieved good photothermal conversion in a concentration-dependent manner for enhancing the effect of ultrasound imaging. In the tumor-bearing nude mice

the photoacoustic signal values in the xenograft were 1.26±0.06 and 0.30±0.01 at 6 h after tail vein injection of AS1411-PLGA@FePc@PFP and PLGA@FePc@PFP

respectively

showing a significant difference in their signal intensity (

0.05);

in vivo

ultrasound/contr

ast imaging also demonstrated a significant difference in the signal value after laser irradiation between the targeted group and the non-targeted group (

0.05). After laser irradiation

the tumor temperature in the targeted nanoparticle group was 50.53±0.56 ℃

as compared with 40.63±0.78 ℃ in the non-targeted group.

Conclusion

A multimodal molecular probe AS1411-PLGA@FePc@PFP for breast cancer has been successfully prepared

which has a good photothermal effect and can be potentially used for photoacoustic/ultrasound multimodal imaging of breast cancer.

关键词

Keywords

references

DESANTIS C E MA J M GAUDET M M Breast cancer statistics, 2019 CA A Cancer J Clin 2019 69 6 438 451 10.3322/caac.21583 DESANTIS C E, MA J M, GAUDET M M, et al . Breast cancer statistics, 2019[J ] . CA A Cancer J Clin, 2019, 69(6): 438-451. DOI: 10.3322/caac.21583.

TURNER N C NEVEN P LOIBL S Advances in the treatment of advanced oestrogen-receptor-positive breast cancer Lancet 2017 389 10087 2403 2414 10.1016/S0140-6736(16)32419-9 TURNER N C, NEVEN P, LOIBL S, et al . Advances in the treatment of advanced oestrogen-receptor-positive breast cancer[J ] . Lancet, 2017, 389(10087): 2403-2414. DOI: 10.1016/S0140-6736(16)32419-9.

SOLANKI M VISSCHER D Pathology of breast Cancer in the last half century Hum Pathol 2019 S0046-S8177 19 30172 8 10.1016/j.humpath.2019.09.007 SOLANKI M, VISSCHER D. Pathology of breast Cancer in the last half century[J]. Hum Pathol, 2019: S0046-S8177(19)30172-8. DOI: 10.1016/j.humpath.2019.09.007.

王志刚多功能超声分子探针显像与增效高强度聚焦超声治疗临床超声医学杂志 2017 19 9 577 579 10.16245/j.cnki.issn1008-6978.2017.09.001 王志刚.多功能超声分子探针显像与增效高强度聚焦超声治疗[J].临床超声医学杂志, 2017, 19(9): 577-579. DOI: 10.16245/j.cnki.issn1008-6978.2017.09.001.

WANG Z G Multifuctional ultrasound molecular probes for imaging and synergistic high-intensity focused ultrasound therapy J Clin Ultrasound Med 2017 19 9 577 579 10.16245/j.cnki.issn1008-6978.2017.09.001 WANG Z G. Multifuctional ultrasound molecular probes for imaging and synergistic high-intensity focused ultrasound therapy[J]. J Clin Ultrasound Med, 2017, 19(9): 577-579. DOI: 10.16245/j.cnki.issn1008- 6978.2017.09.001.

VALLURU K S WILLMANN J K Clinical photoacoustic imaging of cancer Ultrasonography 2016 35 4 267 280 10.14366/usg.16035 VALLURU K S, WILLMANN J K. Clinical photoacoustic imaging of cancer[J]. Ultrasonography, 2016, 35(4): 267-280. DOI: 10.14366/usg.16035.

VALLURU K S WILSON K E WILLMANN J K Photoacoustic imaging in oncology: translational preclinical and early clinical experience Radiology 2016 280 2 332 349 10.1148/radiol.16151414 VALLURU K S, WILSON K E, WILLMANN J K. Photoacoustic imaging in oncology: translational preclinical and early clinical experience[J]. Radiology, 2016, 280(2): 332-349. DOI: 10.1148/radiol.16151414.

SINGH S AGGARWAL A BHUPATHIRAJU N V Glycosylated porphyrins, phthalocyanines, and other porphyrinoids for diagnostics and therapeutics Chem Rev 2015 115 18 10261 10306 10.1021/acs.chemrev.5b00244 SINGH S, AGGARWAL A, BHUPATHIRAJU N V, et al . Glycosylated porphyrins, phthalocyanines, and other porphyrinoids for diagnostics and therapeutics[J ] . Chem Rev, 2015, 115(18): 10261-10306. DOI: 10.1021/acs.chemrev.5b00244.

HAN Z B HAN X ZHAO X M Iron phthalocyanine supported on amidoximated Pan fiber as effective catalyst for controllable hydrogen peroxide activation in oxidizing organic dyes J Hazard Mater 2016 320 27 35 10.1016/j.jhazmat.2016.08.004 HAN Z B, HAN X, ZHAO X M, et al . Iron phthalocyanine supported on amidoximated Pan fiber as effective catalyst for controllable hydrogen peroxide activation in oxidizing organic dyes[J ] . J Hazard Mater, 2016, 320: 27-35. DOI: 10.1016/j.jhazmat.2016.08.004.

LIU J X SHANG T T WANG F J Low-intensity focused ultrasound (LIFU)-induced acoustic droplet vaporization in phase-transition perfluoropentane nanodroplets modified by folate for ultrasound molecular imaging Int J Nanomedicine 2017 12 911 923 10.2147/IJN.S122667 LIU J X, SHANG T T, WANG F J, et al . Low-intensity focused ultrasound (LIFU)-induced acoustic droplet vaporization in phase-transition perfluoropentane nanodroplets modified by folate for ultrasound molecular imaging[J ] . Int J Nanomedicine, 2017, 12: 911-923. DOI: 10.2147/IJN.S122667.

周頔孙阳王志刚包裹液态氟碳的多功能纳米粒研究进展中国医学影像技术 2016 32 1 138 141 10.13929/j.1003-3289.2016.01.036 周頔, 孙阳, 王志刚.包裹液态氟碳的多功能纳米粒研究进展[J].中国医学影像技术, 2016, 32(1): 138-141. DOI: 10.13929/j.1003-3289.2016.01.036.

ZHOU D SUN Y WANG Z G Research progresses of multi-functional liquid perfluorocarbons nanoparticles Chin J Med Imaging Technol 2016 32 1 138 141 10.13929/j.1003-3289.2016.01.036 ZHOU D, SUN Y, WANG Z G. Research progresses of multi-functional liquid perfluorocarbons nanoparticles[J]. Chin J Med Imaging Technol, 2016, 32(1): 138-141. DOI: 10.13929/j.1003-3289.2016.01.036.

SHARMA V R THOMAS S D MILLER D M Nucleolin overexpression confers increased sensitivity to the anti-nucleolin aptamer, AS1411 Cancer Invest 2018 36 9/10 475 491 10.1080/07357907.2018.1527930 SHARMA V R, THOMAS S D, MILLER D M, et al . Nucleolin overexpression confers increased sensitivity to the anti-nucleolin aptamer, AS1411[J ] . Cancer Invest, 2018, 36(9/10): 475-491. DOI: 10.1080/07357907. 2018.1527930.

LIU G GAO N S ZHOU Y Polydopamine-based "four-in-one" versatile nanoplatforms for targeted dual chemo and photothermal synergistic cancer therapy Pharmaceutics 2019 11 10 E507 10.3390/pharmaceutics11100507 LIU G, GAO N S, ZHOU Y, et al . Polydopamine-based "four-in-one" versatile nanoplatforms for targeted dual chemo and photothermal synergistic cancer therapy[J ] . Pharmaceutics, 2019, 11(10): E507. DOI: 10.3390/pharmaceutics11100507.

王征丁劲松罗声娟携带适配体AS1411的液态内核纳米超声造影剂的制备和评估中南大学学报(医学版) 2018 43 6 610 618 10.11817/j.issn.1672-7347.2018.06.006 王征, 丁劲松, 罗声娟, 等.携带适配体AS1411的液态内核纳米超声造影剂的制备和评估[J].中南大学学报(医学版), 2018, 43(6): 610-618. DOI: 10.11817/j.issn.1672-7347.2018.06.006.

WANG Z DING J S LUO S J Development and evaluation of ultrasound contrast agents with AS1411-conjugated nanoparticles with liquid core J Central South Univ Med Sci 2018 43 6 610 618 10.11817/j.issn.1672-7347.2018.06.006 WANG Z, DING J S, LUO S J, et al . Development and evaluation of ultrasound contrast agents with AS1411-conjugated nanoparticles with liquid core[J ] . J Central South Univ Med Sci, 2018, 43(6): 610-618. DOI: 10.11817/j.issn.1672-7347.2018.06.006.

戴碧纯潘碧垚张珊珊核酸适配体技术在胰腺癌诊断和治疗中的应用肝胆胰外科杂志 2019 31 6 325 328 10.11952/j.issn.1007-1954.2019.06.002 戴碧纯, 潘碧垚, 张珊珊, 等.核酸适配体技术在胰腺癌诊断和治疗中的应用[J].肝胆胰外科杂志, 2019, 31(6): 325-328. DOI: 10.11952/j.issn.1007-1954.2019.06.002.

DAI B C PAN B Y ZHANG S S Applicationof nucleic acid aptamer technology in the diagnosis and treatment of pancreatic cancer J Hepatopancreatobil Surg 2019 31 6 325 328 10.11952/j.issn.1007-1954.2019.06.002 DAI B C, PAN B Y, ZHANG S S, et al . Applicationof nucleic acid aptamer technology in the diagnosis and treatment of pancreatic cancer[J ] . J Hepatopancreatobil Surg, 2019, 31(6): 325-328. DOI: 10.11952/j.issn.1007-1954.2019.06.002.

MAEDA H NAKAMURA H FANG J The EPR effect for macromolecular drug delivery to solid tumors: Improvement of tumor uptake, lowering of systemic toxicity, and distinct tumor imaging in vivo Adv Drug Deliv Rev 2013 65 1 71 79 10.1016/j.addr.2012.10.002 MAEDA H, NAKAMURA H, FANG J. The EPR effect for macromolecular drug delivery to solid tumors: Improvement of tumor uptake, lowering of systemic toxicity, and distinct tumor imaging in vivo [J ] . Adv Drug Deliv Rev, 2013, 65(1): 71-79. DOI: 10.1016/j.addr. 2012.10.002.

GHAFFARI H BEIK J TALEBI A New physical approaches to treat cancer stem cells: a review Clin Transl Oncol 2018 20 12 1502 1521 10.1007/s12094-018-1896-2 GHAFFARI H, BEIK J, TALEBI A, et al . New physical approaches to treat cancer stem cells: a review[J ] . Clin Transl Oncol, 2018, 20(12): 1502-1521. DOI: 10.1007/s12094-018-1896-2.

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