Role of leptin up-regulated GRP78 expression in migration of lung cancer cells

Youqile WU; Xiaojing LIN; Jingjing WU; Xuemei LIAN

doi:10.16016/j.1000-5404.201911165

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Role of leptin up-regulated GRP78 expression in migration of lung cancer cells

更新时间：2026-01-09

- Role of leptin up-regulated GRP78 expression in migration of lung cancer cells
- Journal of Third Military Medical University Vol. 42, Issue 8, Pages: 799-806(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81973030);the Special Project of Technology Innovation and Application Development of Chongqing(CSTC2019jscx-msxmX0280)
- DOI：10.16016/j.1000-5404.201911165
  CLC：
- Received：20 November 2019，
  
  Published：30 April 2020
- 稿件说明：
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Youqile WU, Xiaojing LIN, Jingjing WU, et al. Role of leptin up-regulated GRP78 expression in migration of lung cancer cells[J]. Journal of Third Military Medical University, 2020, 42(8): 799-806.
DOI：

Youqile WU, Xiaojing LIN, Jingjing WU, et al. Role of leptin up-regulated GRP78 expression in migration of lung cancer cells[J]. Journal of Third Military Medical University, 2020, 42(8): 799-806. DOI： 10.16016/j.1000-5404.201911165.

摘要

Abstract

Objective

To investigate the effect of leptin on the migration of lung cancer cell lines A549 and H460 and the potential mechanisms.

Methods

The GEPIA website was used to analyze the correlation of the expression levels of leptin and glucose-regulated protein-78 (GRP78) with overall survival rate in lung cancer patients from TCGA database. Wound healing assay was used to detect the effect of leptin and HA15 (GRP78 targeting inhibitor) on the migration of lung cancer A549 and H460 cells; Immunofluorescence assay was used to measure the expression of GRP78 in lung cancer cells. Finally

lung cancer A549 cells were treated with PI3K targeting inhibitor Ly294002

mTOR targeting inhibitor rapamycin alone or in combination with leptin

and then Western blot assay was used to investigate the impact of leptin on PI3K/mTOR/STAT3 signaling pathway and GRP78 expression.

Results

Survival analysis showed that high expression of leptin or GRP78 was closely related to decreased overall survival rate in lung cancer patients (

0.05). Wound healing test indicated that leptin enhanced the migration of lung cancer A549 and H460 cells (

0.05). Immunofluorescence assay displayed that leptin upregulated GRP78 expression in A549 and H460 cells (

0.01). Western blot assay suggested that leptin could upregulate the expression of GRP78 in lung cancer A549 cells through PI3K/mTOR/STAT3 signaling pathway (

0.001). Wound healing test showed inhibition of GRP78 attenuated the effect of leptin on promoting migration.

Conclusion

Leptin promotes the migration of lung cancer cells by up-regulating GRP78 through PI3K/mTOR/STAT3 signaling pathway.

关键词

Keywords

references

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