Dexmedetomidine inhibits systemic inflammatory response induced by renal ischemia reperfusion <italic style="font-style: italic">via</italic> mediating macrophage polarization

Xinhai HE; Xintong CHEN; Ziheng YANG; Li GU; Li ZHOU; Junxian QUAN; Kaizhi LU; Jianteng GU

doi:10.16016/j.1000-5404.201911107

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Dexmedetomidine inhibits systemic inflammatory response induced by renal ischemia reperfusion via mediating macrophage polarization

更新时间：2026-01-09

- Dexmedetomidine inhibits systemic inflammatory response induced by renal ischemia reperfusion via mediating macrophage polarization
- Journal of Third Military Medical University Vol. 42, Issue 6, Pages: 611-615(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81772050);the Basic and Frontier Research Fund of Chongqing(cstc2018jcyjAX0577)
- DOI：10.16016/j.1000-5404.201911107
  CLC：
- Received：13 November 2019，
  
  Published：30 March 2020
- 稿件说明：
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Xinhai HE, Xintong CHEN, Ziheng YANG, et al. Dexmedetomidine inhibits systemic inflammatory response induced by renal ischemia reperfusion via mediating macrophage polarization[J]. Journal of Third Military Medical University, 2020, 42(6): 611-615.
DOI：

Xinhai HE, Xintong CHEN, Ziheng YANG, et al. Dexmedetomidine inhibits systemic inflammatory response induced by renal ischemia reperfusion via mediating macrophage polarization[J]. Journal of Third Military Medical University, 2020, 42(6): 611-615. DOI： 10.16016/j.1000-5404.201911107.

摘要

Abstract

Objective

To explore the effects of dexmedetomidine (Dex) on systemic inflammatory response induced by renal ischemia reperfusion (rIRI) and the possible mechanism.

Methods

Fifteen C57BL/6J mice were randomly divided into 3 groups

that is

sham operation group (laparotomy without renal pedicle occlusion)

rIRI group (bilateral renal pedicles was clamped for 60 min with a microvascular clamp)

and rIRI+Dex group (intraperitoneal injection of 25 μg/ml Dex in 15 min prior to bilateral renal ischemia). In 24 h after surgery or reperfusion

the blood samples were harvested and analyzed for counts of neutrophils

monocytes and lymphocytes by blood analyzer and for the serum levels of inflammatory cytokines (TNF-α

IL-1β and IL-10) by ELISA. Western blotting was used to detect iNOS and Arg 1 in the harvested peritoneal macrophages for the polarization.

Results

Compared with the mice in the sham group

the counts of neutrophils

monocytes and lymphocytes were significantly elevated (

0.01)

the serum levels of TNF-α and IL-1β were increased (

0.01)

and the expression of M1 marker iNOS was enhanced while that of M2 marker Arg 1 was decreased (

0.01). Dex pretreatment resulted in no significant changes in the counts of neutrophils

monocytes and lymphocytes (

0.05)

reduced serum levels of TNF-α and IL-1β (

0.01) and increased level of IL-10 (

0.01)

and decreased expression of iNOS (

0.01) as well as increase of Arg 1 (

0.05) when compared with the indicators in the rIRI group.

Conclusion

Dex inhibits the systemic inflammatory response induced by rIRI

which may be associated with modulation of macrophage polarization.

关键词

Keywords

references

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⁰

Dexmedetomidine inhibits systemic inflammatory response induced by renal ischemia reperfusion via mediating macrophage polarization

DOI：10.16016/j.1000-5404.201911107

摘要

Abstract

关键词

Keywords

references