Copine 8 promotes proliferation, invasion and metastasis of pancreatic cancer

Junfeng ZHANG; Zhiping ZHAO; Huan QIN; Bing NI; Huaizhi WANG

doi:10.16016/j.1000-5404.201911042

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Copine 8 promotes proliferation, invasion and metastasis of pancreatic cancer

更新时间：2026-01-09

- Copine 8 promotes proliferation, invasion and metastasis of pancreatic cancer
- Journal of Third Military Medical University Vol. 42, Issue 6, Pages: 601-610(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Supported by the National Key R & D Program of China(2017YFC1308600);the National Natural Science Foundation of China(81672382);and the Clinical Research Funds of Third Military Medical University(SWH2017ZDCX2004)
- DOI：10.16016/j.1000-5404.201911042
  CLC：
- Received：06 November 2019，
  
  Published：30 March 2020
- 稿件说明：
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Junfeng ZHANG, Zhiping ZHAO, Huan QIN, et al. Copine 8 promotes proliferation, invasion and metastasis of pancreatic cancer[J]. Journal of Third Military Medical University, 2020, 42(6): 601-610.
DOI：

Junfeng ZHANG, Zhiping ZHAO, Huan QIN, et al. Copine 8 promotes proliferation, invasion and metastasis of pancreatic cancer[J]. Journal of Third Military Medical University, 2020, 42(6): 601-610. DOI： 10.16016/j.1000-5404.201911042.

摘要

Abstract

Objective

To explore the biological role of copine 8 in the occurrence and progression of pancreatic cancer.

Methods

GEO datasets were used to compare the expression of copine 8 in pancreatic cancer tissues and adjacent tissues

and TCGA datasets were used to analyze the impact of copine 8 expression on the patients' overall survival rate. The differentially expressed genes between the patients with high and low copine 8 expression were analyzed using GO

KEGG enrichment analysis and gene set enrichment analysis (GSEA). The effect of small interfering RNA-mediated silencing of copine 8 on proliferation

invasion

metastasis

and apoptosis of pancreatic cancer cell lines CFPAC-1 and AsPC-1 were examined using CCK8 assay

colony formation assay

transwell assay

and Annexin V-FITC and propidium iodide staining. The pancreatic cancer transcriptome dataset was analyzed to identify the pathways regulated by copine 8.

Results

Copine 8 mRNA and protein were highly expressed in pancreatic cancer tissues and lowly expressed in adjacent tissues. Copine 8 expression was significantly correlated with the survival prognosis of the patients with pancreatic cancer (

=0.033). Enrichment analysis showed that copine 8 affected WNT

Hippo

mTOR

and ERBB signaling pathways in pancreatic cancer

and may promote the progression of pancreatic cancer by affecting the expression of the key molecules YAP1

STK3

STK4 and SGMS1 in Hippo pathway. Interference of copine 8 expression significantly suppressed the proliferation

invasion and migration and obviously increased the number of apoptotic cells in 2 pancreatic cancer cell lines.

Conclusion

Copine 8 has a strong cancer-promoting effect and plays a significant role in the proliferation

invasion

metastasis and apoptosis of pancreatic cancer.

关键词

Keywords

references

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