Protective effect of troxerutin against spinal cord injury in rats: a transcriptomic study

Mei XIONG; Linbang WANG; Piming NIE; Zhiyu CHEN; Weiyang ZHONG; Zhengxue QUAN

doi:10.16016/j.1000-5404.201910178

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Protective effect of troxerutin against spinal cord injury in rats: a transcriptomic study

更新时间：2026-01-09

- Protective effect of troxerutin against spinal cord injury in rats: a transcriptomic study
- Journal of Third Military Medical University Vol. 42, Issue 6, Pages: 624-631(2020)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201910178
  CLC：
- Received：25 November 2019，
  
  Published：30 March 2020
- 稿件说明：
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Mei XIONG, Linbang WANG, Piming NIE, et al. Protective effect of troxerutin against spinal cord injury in rats: a transcriptomic study[J]. Journal of Third Military Medical University, 2020, 42(6): 624-631.
DOI：

Mei XIONG, Linbang WANG, Piming NIE, et al. Protective effect of troxerutin against spinal cord injury in rats: a transcriptomic study[J]. Journal of Third Military Medical University, 2020, 42(6): 624-631. DOI： 10.16016/j.1000-5404.201910178.

摘要

Abstract

Objective

To investigate the protective effect of troxerutin against spinal cord injury (SCI) in rats and explore the underlying mechanisms.

Methods

Sixty 8-week-old male SD rats were randomly divided into sham operation group

SCI group and troxerutin treatment group. In the sham group

the rats were subjected to laminectomy without SCI; rat models of SCI were established in SCI group and troxerutin treatment group

and in the latter group

the rats received daily intravenous injection of troxerutin after SCI for 4 consecutive weeks. Neurological recovery of the rats was assessed based on the Basso-Beattie-Bresnehan (BBB) score

and HE staining was used to observe the changes in the vacuoles and blood vessels at the injury site of the spinal cord. RNA sequencing was used to examine the differential gene expressions in the injured spinal cord tissue between SCI group and troxerutin group

and Western blotting was performed to verify the differential expression of caspase-6 protein.

Results

Two weeks of troxerutin treatment significantly increased the BBB score of the rats as compared with the rats in SCI group (

0.05)

though the BBB scores in both groups were still lower than that in the sham operation group. At 4 weeks of treatment

the rats in troxerutin group showed decreased vacuoles with increased angiogenesis in the injured spinal cord tissues in comparison with the rats in SCI group. RNA sequencing showed that 261 genes were differentially expressed in the injured spinal cord tissue between troxerutin group and SCI group

including 217 up-regulated and 44 down-regulated genes. GO and KEGG enrichment analysis suggested that troxerutin was capable of activating several signaling pathways including the Wnt

Hippo

TGF-β and Apoptosis pathways

which were functionally related to apoptosis

angiogenesis

neuronal formation

and vasculature. The expression of caspase-6 protein in the semi-dark zone of the injured spinal cord tissues was significantly higher in SCI group than in troxerutin group (

0.05).

Conclusion

Troxerutin shows obvious protective effect against spinal cord injury in rats

possibly by inhibiting cell apoptosis and promoting angiogenesis in the injured spinal cord.

关键词

Keywords

references

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