HBx gene accelerates cell proliferation and enhances inflammatory factors expression through ERK1/2 signaling pathway

Xin HUANG; Siyao ZHANG; Xue WANG; Bin DU; Nan WU; Mengyao ZHOU; Tao FENG

doi:10.16016/j.1000-5404.201910164

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HBx gene accelerates cell proliferation and enhances inflammatory factors expression through ERK1/2 signaling pathway

更新时间：2026-01-09

- HBx gene accelerates cell proliferation and enhances inflammatory factors expression through ERK1/2 signaling pathway
- Journal of Third Military Medical University Vol. 42, Issue 8, Pages: 790-798(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81071770)
- DOI：10.16016/j.1000-5404.201910164
  CLC：
- Received：24 October 2019，
  
  Published：30 April 2020
- 稿件说明：
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Xin HUANG, Siyao ZHANG, Xue WANG, et al. HBx gene accelerates cell proliferation and enhances inflammatory factors expression through ERK1/2 signaling pathway[J]. Journal of Third Military Medical University, 2020, 42(8): 790-798.
DOI：

Xin HUANG, Siyao ZHANG, Xue WANG, et al. HBx gene accelerates cell proliferation and enhances inflammatory factors expression through ERK1/2 signaling pathway[J]. Journal of Third Military Medical University, 2020, 42(8): 790-798. DOI： 10.16016/j.1000-5404.201910164.

摘要

Abstract

Objective

To investigate the effects of HBx gene on hepatocyte cycle and immune system

in vivo

based on self-developed mouse model with stable expression of HBx gene.

Methods

Lentivirus carrying HBx gene were used to infect liver precursor cells 14-19. Then the modified liver precursor cells were injected into the portal vein of mice to construct the model. The model was identified with qRT-PCR

Western blot assay and immunohistochemistry. In 180 d after the model establishment

the mRNA and protein levels of cell cycle regulators CDK4 and Cyclin D1

TNF-α and IL-10 in the liver tissue were detected. While the protein levels of the proteins in the ERK1/2 and MEK1/2 signalling pathways were also measured. HE staining was used to observe the pathological changes in the liver tissue.

Results

The mouse model with stable expression of HBx gene was successfully constructed. Compared with the mice of normal saline group and the EGFP-14-19 group

in the mice in 180 d after the model establishment

HBx significantly up-regulated the mRNA (

0.01) and protein levels of CDK4

Cyclin D1 and TNF-α

but down-regulated the expression of IL-10 at both levels (

0.05). HBx significantly activated ERK1/2 signalling pathway. After the administration of ERK1/2 signal pathway inhibitor U0126

the mRNA and protein expression of CDK4

Cyclin D1

and TNF-α was significantly down-regulated in the experimental group than the control group (

0.05)

but the mRNA and protein levels of IL-10 were in an opposite trend (

0.01). HE staining showed that the liver tissue had obvious pathological changes in the mouse model.

Conclusion

HBx accelerates cell proliferation and induces liver inflammation by activating ERK1/2 signaling pathway.

关键词

Keywords

references

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