Pterostilbene and treadmill exercise induce white adipose browning in rats

Jiawei ZHENG; Wujian LIU; Jundong ZHU; Mantian MI

doi:10.16016/j.1000-5404.201910115

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Pterostilbene and treadmill exercise induce white adipose browning in rats

Basic Medicine | 更新时间：2026-01-09

- Pterostilbene and treadmill exercise induce white adipose browning in rats
- Journal of Third Military Medical University Vol. 42, Issue 4, Pages: 367-374(2020)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队营养与食品卫生学教研室，重庆市营养与食品安全重点实验室，重庆市医学营养研究中心
- 作者简介：
- 基金信息：
  
  the Project of Military Logistic Research(AWS17J014)
- DOI：10.16016/j.1000-5404.201910115
  CLC： Q591.5;R282.71;R339.4
- Received：18 October 2019，
  
  Revised：2019-11-26，
  
  Published：28 February 2020
- 稿件说明：
移动端阅览
Jiawei ZHENG, Wujian LIU, Jundong ZHU, et al. Pterostilbene and treadmill exercise induce white adipose browning in rats[J]. Journal of Third Military Medical University, 2020, 42(4): 367-374.
DOI：

Jiawei ZHENG, Wujian LIU, Jundong ZHU, et al. Pterostilbene and treadmill exercise induce white adipose browning in rats[J]. Journal of Third Military Medical University, 2020, 42(4): 367-374. DOI： 10.16016/j.1000-5404.201910115.

摘要

目的

探讨紫檀芪(pterostilbene，PST)干预和跑台运动对大鼠白色脂肪棕色化的影响及其相关分子机制。

方法

24只8周龄雄性SD大鼠采用随机数字表法分为4组:安静对照组(C)、PST(50 mg/kg)干预组(PST)、跑台运动组(EX)、跑台运动+PST干预组(EX+PST)，干预4周。检测大鼠进食量、体质量和腹股沟白色脂肪(inguinal white adipose tissue，iWAT)质量，HE染色观察iWAT的形态学特征，实时定量PCR和Western blot检测iWAT中白色脂肪棕色化的一些标志基因和调控信号分子的表达。

结果

PST组、EX组、EX+PST组与对照组比较，进食量无明显变化，但体质量增加、iWAT指数明显降低(

0.05)；HE染色显示:各干预组较对照组iWAT脂肪细胞变小、变圆并伴有多室细胞出现，呈棕色化特征；同时，白色脂肪棕色化的标志基因和调控信号分子包括解偶联蛋白1(uncoupling protein 1

UCP1)、诱导细胞死亡DNA片断化因子α样效应因子A (cell death-inducing DNA fragmentation factor alpha-like effector A

CIDEA)、跨膜蛋白26(transmembrane protein 26

TMEM26)、簇分化抗原137(cluster of differentiation 137

CD137)、肉碱棕榈酰转移酶1A (carnitine palmitoyltransferase 1A

CPT1A)、细胞色素C(Cytochrome c，Cyto C)、沉默信息调节因子1(silent information regulator 1

Sirt1)、过氧化物酶体增殖活化受体γ共激活因子1α(peroxisome proliferator activator receptor gamma coactivator 1 alpha，PGC-1α)、R结构域蛋白16(PR domain-containing 16，PRDM16)等mRNA表达显著增加(

0.05)，磷酸化腺苷酸活化蛋白激酶(phosphorylated adenosine monophosphate activated protein kinase，p-AMPK)、UCP1、PRDM16蛋白水平明显升高(

0.05)。且上述变化在EX+PST组中较PST组或EX组更为显著(

0.05)。

结论

紫檀芪和跑台运动单独或联合干预均可诱导大鼠iWAT发生棕色化并降低脂肪蓄积和体质量增加，其机制可能与AMPK信号转导途径激活有关，且二者联合干预效应优于单一因素干预。

Abstract

Objective

To investigate the effects of pterostilbene (PST) intervention and treadmill exercise on browning of white adipose in rats and explore the molecular mechanisms.

Methods

Twenty-four 8-week-old male SD rats were randomly divided into 4 groups: sedentary control group (C)

50 mg/kg PST treatment group

treadmill exercise (EX) group

and treadmill exercise with PST treatment (EX+PST) group. After corresponding treatments for 4 consecutive weeks

the rats were evaluated for changes in food intake

body weight and inguinal white adipose tissue (iWAT) mass. The morphological features of the iWAT were analyzed with HE staining. The mRNA and protein levels of the marker genes and the regulatory molecules for white fat browning in the iWAT were determined using real-time quantitative PCR and Western blotting

respectively.

Results

The body weight gain and iWAT index in the 3 treatment groups were all significantly lower than those of the control group (

0.05)

while food intake did not differ significantly among the 4 groups. Compared with those in the control group

the adipocytes within the iWAT in the 3 treatment groups showed a smaller size and a more spherical morphology accompanied by the presence of cellular compartmentation

which were all characteristics of brown adipocytes. The mRNA and protein levels of the marker genes and regulatory molecules for white adipose browning

including uncoupling protein 1 (UCP1)

cell death-inducing DNA fragmentation factor alpha-like effector A (CIDEA)

cluster of differentiation 137 (CD137)

transmembrane protein 26 (TMEM26)

carnitine palmitoyltransferase 1A (CPT1A)

cytochrome c (Cyto c)

silent information regulator 1 (Sirt1)

peroxisome proliferator activator receptor gamma coactivator 1 alpha (PGC-1α)

PR domain-containing 16 (PRDM16) and phosphorylated adenosine monophosphate activated protein kin

ase (p-AMPK) were all significantly increased in the iWAT in the 3 treatment groups (

0.05). These changes were more obvious in EX+PST group than in EX group and PST group (

0.05).

Conclusion

PST intervention and treadmill exercise

either alone or in combination

can induce the browning of iWAT and reduce fat accumulation and body weight gain in rats through a mechanism possibly involving the activation of AMPK signal transduction pathway. PST combined with treadmill exercise produces more pronounced effects than either PST or treadmill exercise alone.

关键词

Keywords

references

SMITH K B, SMITH M S. Obesity statistics[J]. Prim Care, 2016, 43(1): 121-135, ix. DOI: 10.1016/j.pop.2015.10.001.

CEDIKOVA M, KRIPNEROVÁ M, DVORAKOVA J, et al . Mitochondria in white, brown, and beige adipocytes[J ] . Stem Cells Int, 2016, 2016: 6067349. DOI: 10.1155/2016/ 6067349.

MONTANARI T, POŠĆIĆ N, COLITTI M. Factors involved in white-to-brown adipose tissue conversion and in thermo genesis: a review[J]. Obes Rev, 2017, 18(5): 495-513. DOI: 10.1111/obr.12520.

SPRINGER M, MOCO S. Resveratrol and its human metabolites- effects on metabolic health and obesity[J]. Nutrients, 2019, 11(1): E143. DOI: 10.3390/nu11010143.

WANG S, LIANG X, YANG Q, et al . Resveratrol induces brown-like adipocyte formation in white fat through activation of AMP-activated protein kinase (AMPK) α1[J ] . Int J Obes (Lond), 2015, 39(6): 967-976. DOI: 10.1038/ijo.2015.23.

LIAO W Y, YIN X H, LI Q R, et al . Resveratrol-induced white adipose tissue Browning in obese mice by remodeling fecal Microbiota[J ] . Molecules, 2018, 23(12): E3356. DOI: 10.3390/molecules23123356.

WANG P, SANG S M. Metabolism and pharmacokinetics of resveratrol andpterostilbene[J]. Biofactors, 2018, 44(1): 16-25. DOI: 10.1002/biof.1410.

AYTON A, IBRAHIM A. Obesity is a public health emergency[J]. BMJ, 2019, 366: l5463. DOI: 10.1136/bmj.l5463.

CHECHI K, NEDERGAARD J, RICHARD D. Brown adipose tissue as an anti-obesity tissue in humans[J]. Obes Rev, 2014, 15(2): 92-106. DOI: 10.1111/obr.12116.

[JANKOVIC A, OTASEVIC V, STANCIC A, et al . Physiological regulation and metabolic role of Browning in white adiposetissue[J ] . Horm Mol Biol Clin Investig, 2017, 31(1).pii: /j/hmbci.2017.31.issue-/j/hmbci.2017.31.issu1/hmbci-2017-0034/hmbci-2017-0034.xml. DOI: 10.1515/hmbci-2017-0034 http://dx.doi.org/10.1515/hmbci-2017-0034 ..

[THYAGARAJAN B, FOSTER M T.Beiging of white adipose tissue as a therapeutic strategy for weight loss in humans[J ] . Horm Mol Biol Clin Investig, 2017, 31(2).pii: /j/hmbci.2017.31.issue-/j/hmbci.2017.31.issu2/hmbci-2017-0016/hmbci-2017-0016.xml. DOI: 10.1515/hmbci-2017-0016 http://dx.doi.org/10.1515/hmbci-2017-0016 .

ALDISS P, BETTS J, SALE C, et al . Exercise-induced 'browning' of adipose tissues[J ] . Metab Clin Exp, 2018, 81: 63-70. DOI: 10.1016/j.metabol.2017.11.009.

FERNÁNDEZ-QUINTELA A, MILTON-LASKIBAR I, GONZÁLEZ M, et al .Antiobesity effects of resveratrol: which tissues are involved?[J ] . Ann N Y Acad Sci, 2017, 1403(1): 118-131. DOI: 10.1111/nyas.13413.

SMOLIGA J M, BLANCHARD O. Enhancing the delivery of resveratrol in humans: if low bioavailability is the problem, what is the solution?[J]. Molecules, 2014, 19(11): 17154-17172. DOI: 10.3390/molecules191117154.

DEWAL R S, STANFORD K I. Effects of exercise on brown and beigeadipocytes[J]. Biochim Biophys Acta Mol Cell Biol Lipids, 2019, 1864(1): 71-78. DOI: 10.1016/j.bbalip. 2018.04.013.

CHEN L H, CHIEN Y W, LIANG C T, et al . Green tea extract induces genes related to Browning of white adipose tissue and limits weight-gain in high energy diet-fed rat[J ] . Food Nutr Res, 2017, 61(1): 1347480. DOI: 10.1080/16546628.2017.1347480.

RODRIGUEZ LANZI C, PERDICARO D J, LANDA M S, et al . Grapepomace extract induced beige cells in white adipose tissue from rats and in 3T3-L1 adipocytes[J ] . J Nutr Biochem, 2018, 56: 224-233. DOI: 10.1016/j.jnutbio. 2018.03.001.

GUO YY, LI B Y, PENG W Q, et al . Taurine-mediated Browning of white adipose tissue is involved in its anti-obesity effect in mice[J ] . J Biol Chem, 2019, 294(41): 15014-15024. DOI: 10.1074/jbc.RA119.009936.

[BARGUT T C L, SOUZA-MELLO V, AGUILA M B, et al . Browning of white adipose tissue: lessons from experimentalmodels[J ] . Horm Mol Biol Clin Investig, 2017, 31(1).pii: /j/hmbci.2017.31.issue-/j/hmbci.2017.31.issu1/hmbci-2016-0051/hmbci-2016-0051.xml. DOI: 10.1515/hmbci-2016-0051 http://dx.doi.org/10.1515/hmbci-2016-0051 ..

ZHANG J, WU H, MA S, et al . Transcription regulators and hormones involved in the development of brown fat and w hite fat Browning: transcriptional and hormonal control of brown/beige fat development[J ] . Physiol Res, 2018, 67(3): 347-362. DOI: 10.33549/physiolres. 933650.

YANG Q Y, LIANG X W, SUN X F, et al . AMPK/α-ketoglutarate axis dynamically mediates DNA demethylation in the Prdm16 promoter and brown adipogenesis[J ] . Cell Metab, 2016, 24(4): 542-554. DOI: 10.1016/j.cmet.2016. 08.010.

DESJARDINS E M, STEINBERG G R. Emerging role of AMPK in brown and beige adipose tissue (BAT): implications for obesity, insulin resistance, and type 2 diabetes[J]. Curr Diab Rep, 2018, 18(10): 80. DOI: 10.1007/s11892-018-1049-6.

THIRUPATHI A, DE SOUZA C T. Multi-regulatory network of ROS: the interconnection of ROS, PGC-1 alpha, and AMPK-SIRT1 duringexercise[J]. J Physiol Biochem, 2017, 73(4): 487-494. DOI: 10.1007/s13105-017-0576-y.

WANG X H, ZHU L, HONG X, et al . Resveratrol attenuated TNF-α-induced MMP-3 expression in human nucleuspulposus cells by activating autophagy via AMPK/SIRT1 signaling pathway[J ] . Exp Biol Med (Maywood), 2016, 241(8): 848-853. DOI: 10.1177/1535370216637940..

LI J, YU S P, YING J, et al . Resveratrol prevents ROS-induced apoptosis in high glucose-treated retinal capillary endothelial cells via the activation of AMPK/Sirt1/PGC-1α pathway[J ] . Oxid Med Cell Longev, 2017, 2017: 7584691. DOI: 10.1155/2017/7584691..

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