Effect of fibroblast growth factor 21 on migration and invasion of human pancreatic cancer cells in an inflammatory environment

Wei XIAO; Shasha ZHU; Panchun ZHENG; Yaying YANG

doi:10.16016/j.1000-5404.201909197

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Effect of fibroblast growth factor 21 on migration and invasion of human pancreatic cancer cells in an inflammatory environment

Basic Medicine | 更新时间：2026-01-09

- Effect of fibroblast growth factor 21 on migration and invasion of human pancreatic cancer cells in an inflammatory environment
- Journal of Third Military Medical University Vol. 42, Issue 4, Pages: 414-420(2020)
- 作者机构：
  
  1.400016 重庆，重庆医科大学:分子医学诊断中心病理教研室
  2.400016 重庆，重庆医科大学:附属第一医院临床分子医学检测中心
- 作者简介：
- 基金信息：
  
  the Sci-Tech Research Project of Chongqing Municipal Education Commission(KJ1702016)
- DOI：10.16016/j.1000-5404.201909197
  CLC： R341; R364.5; R735.9
- Received：26 September 2019，
  
  Revised：2019-12-06，
  
  Published：28 February 2020
- 稿件说明：
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Wei XIAO, Shasha ZHU, Panchun ZHENG, et al. Effect of fibroblast growth factor 21 on migration and invasion of human pancreatic cancer cells in an inflammatory environment[J]. Journal of Third Military Medical University, 2020, 42(4): 414-420.
DOI：

Wei XIAO, Shasha ZHU, Panchun ZHENG, et al. Effect of fibroblast growth factor 21 on migration and invasion of human pancreatic cancer cells in an inflammatory environment[J]. Journal of Third Military Medical University, 2020, 42(4): 414-420. DOI： 10.16016/j.1000-5404.201909197.

摘要

目的

探讨成纤维细胞生长因子21(fibroblast growth factor 21，FGF21)在胰腺癌中的表达及其对炎症环境下胰腺癌细胞迁移和侵袭的影响。

方法

采用免疫组化检测FGF21在胰腺癌和正常胰腺组织中的表达。将人胰腺癌细胞Capan-1和AsPC-1分为4组:对照组、FGF21组、LPS组和FGF21+LPS组。划痕和Transwell实验检测各组细胞迁移和侵袭能力。RT-qPCR和Western blot分别检测IL-6、MMP-9 mRNA和蛋白的表达。

结果

胰腺癌组织中FGF21表达明显低于正常胰腺组织(

0.05)。与LPS组相比较，FGF21+LPS组细胞的迁移和侵袭能力均显著减弱(

0.05)。RT-qPCR和Western blot结果显示:FGF21+LPS组IL-6、MMP-9 mRNA和MMP-9蛋白表达均显著低于LPS组(

0.05)。

结论

FGF21在胰腺癌组织中呈低表达，FGF21干预后能显著抑制LPS引起的胰腺癌细胞迁移和侵袭能力增高，其机制可能与FGF21抑制IL-6和MMP-9的表达有关。

Abstract

Objective

To investigate the expression of fibroblast growth factor 21 (FGF21) in pancreatic cancer and its effect on migration and invasion of pancreatic cancer cells in an inflammatory environment.

Methods

FGF21 expression was detected in 60 specimens of pancreatic cancer tissues and 55 normal pancreatic tissues using immunohischemistry. Two human pancreatic cancer cell lines (Capan-1 and AsPC-1) were both divided into control group

FGF21 group

lipopolysaccharide (LPS) group and FGF21+LPS group. After corresponding treatment

the cell migration and invasion were evaluated using scratch wound healing assay and Transwell assay. RT-qPCR and Western blotting were used to examine the expression levels of IL-6 and MMP-9 mRNA and MMP-9 protein in the cells.

Results

The expression levels of FGF21 were significantly lower in pancreatic cancer tissues than in normal pancreatic tissues (

0.05). Compared with LPS-treated cells

the cells treated with both LPS and FGF21 showed significantly lowered migration and invasion abilities (

0.05) and decreased expression levels of IL-6 and MMP-9 mRNA and MMP-9 protein (

0.05).

Conclusion

FGF21 expression is significantly decreased in pancreatic cancer tissues. Intervention with FGF21 can significantly inhibit LPS-induced enhancement of migration and invasion of pancreatic cancer cells possibly by inhibiting IL-6 and MMP-9 expression.

关键词

Keywords

references

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