Effects of hypoxia on expression of formyl peptide receptor 1 in rat lung fibroblasts <italic style="font-style: italic">in vitro</italic>

Xiaoxu LI; Jian HUANG; Li JIAO; Yu CUI; Chengzhong YANG; Ming WEI

doi:10.16016/j.1000-5404.201909154

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Effects of hypoxia on expression of formyl peptide receptor 1 in rat lung fibroblasts in vitro

更新时间：2026-01-09

- Effects of hypoxia on expression of formyl peptide receptor 1 in rat lung fibroblasts in vitro
- Journal of Third Military Medical University Vol. 42, Issue 6, Pages: 568-575(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Youth Training Plan of Military Medical Science and Technology(20QNPY002);the Key Special Program of "Thirteenth Five-year Plan" of Logistic Scientific Research of PLA(BWS17J031)
- DOI：10.16016/j.1000-5404.201909154
  CLC：
- Received：20 September 2019，
  
  Published：30 March 2020
- 稿件说明：
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Xiaoxu LI, Jian HUANG, Li JIAO, et al. Effects of hypoxia on expression of formyl peptide receptor 1 in rat lung fibroblasts in vitro[J]. Journal of Third Military Medical University, 2020, 42(6): 568-575.
DOI：

Xiaoxu LI, Jian HUANG, Li JIAO, et al. Effects of hypoxia on expression of formyl peptide receptor 1 in rat lung fibroblasts in vitro[J]. Journal of Third Military Medical University, 2020, 42(6): 568-575. DOI： 10.16016/j.1000-5404.201909154.

摘要

Abstract

Objective

To study the effect of hypoxia on the expression of formyl peptide receptor 1 (FPR1) in rat lung fibroblasts.

Methods

We tested the chemotactic activity of 10

100

and 1 000 nmol/L FPR agonist (fMLF) for lung fibroblasts isolated from adult female SD rats using Boyden chemotaxis chamber assay. The cells were cultured in a hypoxic incubator (1% O

5% CO

and 94% N

) at 37 ℃ for 24 h

and the culture supernatant was collected and treated with the FPR-specific antagonist tBoc before assessing fibroblast chemotactic activity mediated by FPR. Rat lung fibroblasts cultured in a normoxic condition or in a hypoxic incubator (1% O

) for 2

and 24 h were tested for mRNA and protein levels of FPR1 and FPR agonist annexin A1 (AnxA1) using real-time PCR and Western blotting. The protein levels of FPR1 and AnxA1 were also e

xamined in cells treated with different concentrations of fMLF and in cells treated with tBoc prior to hypoxia for 24 h.

Results

fMLF induced obvious chemotactic migration of rat lung fibroblasts

and 100 nmol/L exhibited the strongest chemotactic activity for the fibroblasts (

0.05). The supernatant of the cells with hypoxic exposure showed a significantly higher chemotactic activity than that of the cells cultured in a normoxic condition (

0.05); the addition of tBoc significantly decreased in the chemotactic activity of the supernatants

and the reduction was more obvious with the hypoxic cell culture supernatant (

0.05). Hypoxia for 12 h significantly increased FPR1 protein expression in the lung fibroblasts (

0.05); hypoxia for 12 and 24 h significantly enhanced the protein expression of AnxA1 in the fibroblasts (

0.05). The mRNA levels of FPR1 and AnxA1 increased significantly in the lung fibroblasts after hypoxia for 2

and 24 h (

0.05); increasing the concentration of fMLF in the cell culture resulted in progressively increased protein levels of FPR1 and AnxA1

and their increments became significant after treatment with fMLF at 100 and 1 000 nmol/L (

0.05); this effect was significantly attenuated by the application of the FPR antagonist tBoc.

Conclusion

FPR1 is expressed in primary cultured rat lung fibroblasts. Hypoxia promotes the expression and secretion of FPR1 and its agonists in rat lung fibroblasts

and FPR1 activation further enhances the expression and secretion of the receptor and its agonists.

关键词

Keywords

references

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Effects of hypoxia on expression of formyl peptide receptor 1 in rat lung fibroblasts in vitro

DOI：10.16016/j.1000-5404.201909154

摘要

Abstract

关键词

Keywords

references