Evaluation on clinical application of mCIM and eCIM in detection of cabapenemase from <italic style="font-style: italic">Enterobacteriaceae Bateremia</italic>

Yonghong WANG; Hang LIU; Jiaqi ZOU; Hualan MA; Yun XIA

doi:10.16016/j.1000-5404.201909092

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Evaluation on clinical application of mCIM and eCIM in detection of cabapenemase from Enterobacteriaceae Bateremia

Basic Medicine | 更新时间：2026-01-09

- Evaluation on clinical application of mCIM and eCIM in detection of cabapenemase from Enterobacteriaceae Bateremia
- Journal of Third Military Medical University Vol. 42, Issue 5, Pages: 466-472(2020)
- 作者机构：
  
  1.400016 重庆，重庆医科大学附属第一医院检验科
  2.409099 重庆，重庆市黔江中心医院检验科
- 作者简介：
- 基金信息：
  
  Supported by the Project of Medical Research of Chongqing Science and Technology Commission and Chongqing Health Commission(2018MSXM104)
- DOI：10.16016/j.1000-5404.201909092
  CLC： R378.2;R446.9;R915
- Received：12 September 2019，
  
  Revised：2020-01-02，
  
  Published：15 March 2020
- 稿件说明：
移动端阅览
Yonghong WANG, Hang LIU, Jiaqi ZOU, et al. Evaluation on clinical application of mCIM and eCIM in detection of cabapenemase from Enterobacteriaceae Bateremia[J]. Journal of Third Military Medical University, 2020, 42(5): 466-472.
DOI：

Yonghong WANG, Hang LIU, Jiaqi ZOU, et al. Evaluation on clinical application of mCIM and eCIM in detection of cabapenemase from Enterobacteriaceae Bateremia[J]. Journal of Third Military Medical University, 2020, 42(5): 466-472. DOI： 10.16016/j.1000-5404.201909092.

摘要

目的

评价改良碳青霉烯灭活试验(modified carbapenem inactivation method

mCIM)和EDTA碳青霉烯灭活试验(EDTA-carbapenem inactivation method

eCIM)对肠杆菌科细菌碳青霉烯酶的检测性能。

方法

收集2017年1月至2018年9月重庆市16家区县医院临床分离的136株碳青霉烯耐药或敏感的肠杆菌科细菌，采用PCR方法检测碳青霉烯酶基因，mCIM和eCIM筛选碳青霉烯酶表型，并对基因检测结果与表型筛选试验结果进行分析和评价。

结果

85株碳青霉烯耐药肠杆菌科细菌中79株检出碳青霉烯酶基因，共表达A类和B类碳青霉烯酶基因8株，占比10.13%(8/79)，碳青霉烯酶表型筛选试验mCIM阳性78株，eCIM阳性44株。51株碳青霉烯敏感菌株未检测到碳青霉烯耐药基因且mCIM均为阴性。mCIM筛选肠杆菌科细菌碳青霉烯酶的灵敏度为98.7%(95%

:92.2~99.9)，特异性为100.0%(95%

:92.1~100.0)，Kappa值为0.985；eCIM筛选金属酶灵敏度为85.7%(95%

:72.1~93.6)，特异性为94.4%(95%

:80.0~99.0)，Kappa值为0.787；eCIM筛选丝氨酸酶灵敏度为89.5%(95%

:74.3~96.6)，特异性为100.0%(95%

:90.6~100.0)，Kapp

a值为0.904。

结论

mCIM联合eCIM检测可有效筛选产碳青霉烯酶菌株，但随着共表达A类和B类碳青霉烯酶菌株的分离率增加会降低对金属酶筛选的灵敏度。

Abstract

Objective

To evaluate the performance of modified carbapenem inactivation method (mCIM) and EDTA carbapenem inactivation method (eCIM) in detection of carbapenemase in

Enterobacteriaceae Bateremia.

Methods

Polymerase chain reaction (PCR) was used to detect carbapenemase gene in 136 strains of carbapenem-resistant and -sensitive

Enterobacteriaceae

clinically isolated from 16 county-level hospital during January 2017 and September 2018. Meanwhile

the phenotype of obtained carbapenemase gene was screened by mCIM and eCIM. The results of gene detection and phenotypic screening test were analyzed and evaluated.

Results

Seventy-nine strains out of 85 carbapenem-resistant

Enterobacteriaceae

strains (CRE) were positive to carbapenemase gene by PCR

and 8 strains expressed class A and B carbapenemase gene

accounting for 10.13% (8/79). Seventy-eight strains of 85 CRE strains were positive detected by mCIM

and 44 strains were identified to be positive by eCIM among them. The carbapenem-resistant genes were not detected in the 51 carbapenem-sensitive strains by mCIM results. Screening the carbapenemase by mCIM had the sensitivity of 98.7% (95%

: 92.2~99.9)

the specificity of 100.0% (95%

: 92.1~100.0)

and the Kappa value of 0.985. When eCIM screened metallo-β-lactamase

the sensitivity was 85.7% (95%

: 72.1~93.6)

the specificity was 94.4%(95%

: 80.0~99.0)

and the Kappa value was 0.787. Screening serine carbapenemase

the sensitivity of eCIM was 89.5%(95%

: 74.3~96.6)

the specificity was 100.0%(95%

: 90.6~100.0)

and the Kappa value was 0.904.

Conclusion

Combination of mCIM and eCIM can screen carbapenemase-producing

Enterobacteriaceae

effectively. However

the sensitivity of the combined detection to screen metallo-β-lactamase will be reduced with the emergence of a large number of co-expressed class A and B carbapenemase-producing

Enterobacteriaceae

关键词

Keywords

references

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Evaluation on clinical application of mCIM and eCIM in detection of cabapenemase from Enterobacteriaceae Bateremia

DOI：10.16016/j.1000-5404.201909092

摘要

Abstract

关键词

Keywords

references