Protective effect of citrus flavone against acute hypoxia in cultured human intestinal smooth muscle cells and the mechanism

Jingjun LIU; Xiaoming WANG; Chengfeng SHEN; Bingnan DENG; Demei CHEN; Lingling PU; Weili LIU; Zhaoli CHEN; Xinxing WANG

doi:10.16016/j.1000-5404.201908220

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Protective effect of citrus flavone against acute hypoxia in cultured human intestinal smooth muscle cells and the mechanism

更新时间：2026-01-09

- Protective effect of citrus flavone against acute hypoxia in cultured human intestinal smooth muscle cells and the mechanism
- Journal of Third Military Medical University Vol. 42, Issue 6, Pages: 594-600(2020)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(31971106);Supported by the General Program of National Natural Science Foundation of China(81372124);BWS(18CXZ044);BWS(16J010)
- DOI：10.16016/j.1000-5404.201908220
  CLC：
- Received：31 August 2019，
  
  Published：30 March 2020
- 稿件说明：
移动端阅览
Jingjun LIU, Xiaoming WANG, Chengfeng SHEN, et al. Protective effect of citrus flavone against acute hypoxia in cultured human intestinal smooth muscle cells and the mechanism[J]. Journal of Third Military Medical University, 2020, 42(6): 594-600.
DOI：

Jingjun LIU, Xiaoming WANG, Chengfeng SHEN, et al. Protective effect of citrus flavone against acute hypoxia in cultured human intestinal smooth muscle cells and the mechanism[J]. Journal of Third Military Medical University, 2020, 42(6): 594-600. DOI： 10.16016/j.1000-5404.201908220.

摘要

Abstract

Objective

To investigate the protective effect of citrus flavone on cultured human intestinal smooth muscle cells (HISMCs) against acute hypoxia and explore the mitochondrial mechanism.

Methods

Cultured HISMCs were exposed to acute hypoxia with 1%

or 5% O

(5% CO

94% N

) for 6 h or with 1% O

for 3

and 9 h. The cells cultured in 1% O

(5% CO

94% N

) were treated with citrus flavonoids (300 or 600 μg/mL) for 6 h. Mitochondrial membrane potential and the cell apoptotic rate were determined with flow cytometry

and the activities of caspase-3 and caspase-8 were detected using Ac-DEVD-pNA and AC-IETD-pNA colorimetry

respectively. The total content of TR3 nuclear orphan receptor

(TR3) in the hypoxic cells and its content in the mitochondria were determined with Western blotting. We also tested the effect of TR3 overexpression on the protective effect of 600 μg/mL citrus flavonoids in the HISMCs exposed to 1% O

Results

Acute hypoxia resulted in significantly decreased mitochondrial membrane potential

enhanced caspase-3 and caspase-8 activities

and increased apoptosis in cultured HISMCs (

0.05). Citrus flavonoids effectively enhanced mitochondrial membrane potential level

suppressed the mitochondrial apoptotic pathway (

0.05)

and inhibited the translocation of TR3 to the mitochondria in the hypoxic cells. Overexpression of TR3 significantly attenuated the protective effect of citrus flavonoids on the hypoxic cells (

0.05).

Conclusion

Citrus flavonoids protect intestinal smooth muscle cells against hypoxic injury by regulating mitochondrial translocation of TR3.

关键词

Keywords

references

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