Different methods for detecting serum markers in patients with acute graft versus host disease

Ting CHEN; Xiaoping LI; Qiangguo GAO; Lei GAO; Yao LIU; Cheng ZHANG; Peiyan KONG; Li GAO; Yimei FENG; Jun RAO; Xi ZHANG

doi:10.16016/j.1000-5404.201907252

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Different methods for detecting serum markers in patients with acute graft versus host disease

更新时间：2026-01-09

- Different methods for detecting serum markers in patients with acute graft versus host disease
- Journal of Third Military Medical University Vol. 41, Issue 23, Pages: 2280-2284(2019)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Key Special Project of National Key R & D Program(2017YFA0105502);the Special Project of Science and Technology Innovation for Social Undertakings and Livelihood Guarantee of Chongqing(CSTC2016shms-ztzx10003)
- DOI：10.16016/j.1000-5404.201907252
  CLC：
- Received：31 July 2019，
  
  Published：25 December 2019
- 稿件说明：
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Ting CHEN, Xiaoping LI, Qiangguo GAO, et al. Different methods for detecting serum markers in patients with acute graft versus host disease[J]. Journal of Third Military Medical University, 2019, 41(23): 2280-2284.
DOI：

Ting CHEN, Xiaoping LI, Qiangguo GAO, et al. Different methods for detecting serum markers in patients with acute graft versus host disease[J]. Journal of Third Military Medical University, 2019, 41(23): 2280-2284. DOI： 10.16016/j.1000-5404.201907252.

摘要

Abstract

Objective

To clarify the consistency of the results of the 2 methods for detecting serum biomarkers in patients with acute graft versus host disease (aGVHD) in order to screen out biomarkers with clinical value and to establish a biomarker screening process for aGVHD.

Methods

Sixty patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT) in our medical center from April 2016 to April 2018 were recruited in this study. Protein chip and ELISA were used to detect the serum cytokine levels in the patients with aGVHD (

=30) and those without (control group

=30).

Results

Both detection methods showed that the serum levels of IL-7 and IL-27p were significantly higher (

0.05)

while the expression level of CD127 was decreased (

0.05) in the aGVHD group than the control. Th

e expression level of IL-27 was correlated with the severity of aGVHD (

0.05). The area under the receiver operating characteristic (ROC) curve was between 0.7 and 0.9

with no significant differences between the 2 methods.

Conclusion

Both protein chip and ELISA have high accuracy and consistency in the detection of serum biomarkers in aGVHD patients. It is possible to establish a high-throughput screening of clinically significant cytokines by protein chip

and then expanding the sample size and repeating the validation by ELISA

which is conducive to clinical development.

关键词

Keywords

references

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