Long non-coding RNA LINC02163 is up-regulated and promotes cell migration and invasion in hepatocellular carcinoma

Yi LUO; Xaoyu FANG; Weijia XIE; Zhengyu ZHOU; Youhao WANG; Xiaojun WANG; Yan JIANG; Xiangyu MA

doi:10.16016/j.1000-5404.201907251

您当前的位置：

首页 >

文章列表页 >

Long non-coding RNA LINC02163 is up-regulated and promotes cell migration and invasion in hepatocellular carcinoma

Clinical Medicine | 更新时间：2026-01-09

- Long non-coding RNA LINC02163 is up-regulated and promotes cell migration and invasion in hepatocellular carcinoma
- Journal of Third Military Medical University Vol. 42, Issue 1, Pages: 81-87(2020)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学):军事预防医学系军队流行病学教研室
  2.400038 重庆，陆军军医大学(第三军医大学):第一附属医院全军肝胆外科研究所
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81302498)
- DOI：10.16016/j.1000-5404.201907251
  CLC： R394.3;R730.23;R735.7
- Received：31 July 2019，
  
  Revised：2019-09-21，
  
  Published：15 January 2020
- 稿件说明：
移动端阅览
Yi LUO, Xaoyu FANG, Weijia XIE, et al. Long non-coding RNA LINC02163 is up-regulated and promotes cell migration and invasion in hepatocellular carcinoma[J]. Journal of Third Military Medical University, 2020, 42(1): 81-87.
DOI：

Yi LUO, Xaoyu FANG, Weijia XIE, et al. Long non-coding RNA LINC02163 is up-regulated and promotes cell migration and invasion in hepatocellular carcinoma[J]. Journal of Third Military Medical University, 2020, 42(1): 81-87. DOI： 10.16016/j.1000-5404.201907251.

摘要

目的

研究长链非编码RNA LINC02163在肝细胞肝癌(hepatocellular carcinoma，HCC)组织及肝癌细胞系中的表达及对肝癌细胞迁移、侵袭的影响。

方法

利用Quantitative Real-time PCR(qRT-PCR)检测LINC02163在5株肝癌细胞系(HuH-7、HCCLM3、MHCC-97H、SNU-182和SMMC-7721)、正常肝细胞系(L02)和60例肝癌组织及其配对癌旁组织中的差异表达情况，分析LINC02163的表达水平与肝癌患者临床病理参数的关系；利用Lipofectamine

2000转染LINC02163小干扰RNA(siRNA)至HuH-7、HCCLM3肝癌细胞内敲低LINC02163表达；通过Transwell实验检测敲低LINC02163后肝癌细胞迁移、侵袭能力的改变。

结果

LINC02163在肝癌组织中的表达水平明显高于配对癌旁组织(

0.001)；LINC02163在肝癌组织中的表达与TNM分期、微血管侵犯(MVI)明显相关(

0.01)；与正常肝细胞系(L02)比较，LINC02163在5株肝癌细胞系中高表达(

0.001)，其中在HuH-7细胞系中表达最高，其次是HCCLM3细胞系；肝癌细胞HuH-7、HCCLM3敲低LINC02163的表达后迁移和侵袭能力明显降低(

0.01)。

结论

LINC02163在肝癌组织和肝癌细胞系中的表达上调，其可促进肝癌细胞的迁移和侵袭，故可能与肝癌的发生、发展密切相关。

Abstract

Objective

To investigate the expression of long non-coding RNA LINC02163 in hepatocellular carcinoma (HCC) tissues and HCC cell lines

and its effect on the migration and invasion of hepatocellular carcinoma cells.

Methods

The differential expression level of LINC02163 in 5 HCC cell lines (HuH-7

HCCLM3

MHCC-97H

SNU-182 and SMMC-7721)

normal liver cell line (L02) and 60 HCC tissues and paired adjacent tissues was determined by quantitative real-time PCR (qRT-PCR)

and the relationship between its expression level and the clinicopathological parameters of HCC patients was analyzed. Lipofectamine

2000 was used to transfect LINC02163 small interfering RNA (siRNA) into HCC HuH-7 and HCCLM3 cells in order to knockdown the expression. Then the changes of migration and invasion ability of HCC cells after the knock-down of LINC02163 were examined by Transwell assay.

Results

The expression level of LINC02163 in HCC tissues was significantly higher than that in paired adjacent tissues (

0.001)

and the level was significantly correlated with TNM stage and microvascular invasion (MVI) in the patients (all

0.01). Compared with normal liver cell line (L02)

LINC02163 was highly expressed in 5 HCC cell lines (

0.001)

among which HuH-7 cells had the highest expression level

followed by HCCLM3 cells. LINC02163 knock-down significantly reduced the migration and invasion abilities in HuH-7 and HCCLM3 cells (

0.01).

Conclusion

LINC02163 is up-regulated in HCC tissues and cell lines and promotes the migration and invasion of HCC cells

which may be closely associated with the occurrence and development of HCC.

关键词

Keywords

references

CHEN W, ZHENG R, BAADE P D, et al . Cancer statistics in China, 2015[J ] . CA Cancer J Clin, 2016, 66(2): 115-132. DOI:10.3322/caac.21338.

WANG F S, FAN J G, ZHANG Z, et al . The global burden of liver disease: the major impact of China[J ] . Hepatology, 2014 2099-2108. DOI:10.1002/hep.27406.

FORNER A, REIG M, BRUIX J. Hepatocellular carcinoma[J]. Lancet, 2018, 391(10127): 1301-1314. DOI:10.1016/S0140-6736(18)30010-2.

SHIH J W, KUNG H J. Long non-coding RNA and tumor hypoxia: new players ushered toward an old arena[J]. J Biomed Sci, 2017, 24(1): 53. DOI:10.1186/s12929-017-0358-4.

SHI X, SUN M, LIU H, et al . Long non-coding RNAs: a new frontier in the study of human diseases[J ] . Cancer Lett, 2013, 339(2): 159-166. DOI:10.1016/j.canlet.2013.06.013.

WEIDLE U H, BIRZELE F, KOLLMORGEN G, et al . Long non-coding RNAs and their role in metastasis[J ] . Cancer Genom Proteom, 2017, 14(3): 143-160. DOI:10.21873/cgp.20027.

YUAN J H, YANG F, WANG F, et al . A long noncoding RNA activated by TGF-beta promotes the invasion-metastasis cascade in hepatocellular carcinoma[J ] . Cancer Cell, 2014, 25(5): 666-681. DOI:10.1016/j.ccr.2014.03.010.

YANG T, HE X, CHEN A, et al . LncRNA HOTAIR contributes to the malignancy of hepatocellular carcinoma by enhancing epithelial-mesenchymal transition via sponging miR-23b-3p from ZEB1[J ] . Gene, 2018, 670: 114-122. DOI:10.1016/j.gene.2018.05.061.

YUAN S X, YANG F, YANG Y, et al . Long noncoding RNA associated with microvascular invasion in hepatocellular carcinoma promotes angiogenesis and serves as a predictor for hepatocellular carcinoma patients' poor recurrence-free survival after hepatectomy[J ] . Hepatology, 2012, 56(6): 2231-2241. DOI:10.1002/hep.25895.

ZHANG L, YANG F, YUAN J H, et al . Epigenetic activation of the MiR-200 family contributes to H19-mediated metastasis suppression in hepatocellular carcinoma[J ] . Carcinogenesis, 2013, 34(3): 577-586. DOI:10.1093/carcin/bgs381.

DONG L, HONG H, CHEN X, et al . LINC02163 regulates growth and epithelial-to-mesenchymal transition phenotype via miR-593-3p/FOXK1 axis in gastric cancer cells[J ] . Artif Cells Nan omed Biotechnol, 2018, 46(sup2): 607-615. DOI:10.1080/21691401.2018.1464462..

周征宇, 向颖, 袁帅, 等.长链非编码RNA LINC00324对非小细胞肺癌细胞生物学功能的影响[J].第三军医大学学报, 2019, 41(4): 331-338. DOI: 10.16016/j.1000-5404.201811031.

ZHOU Z Y, XIANG Y, YUAN S, et al . Effect of long non- coding RNA LINC00324 on biological functions of non-small cell lung cancer cells[J ] . J Third Mil Med Univ, 2019, 41(4) : 331-338. DOI: 10.16016/j.1000-5404.201811031.

OU H, LIU X, XIANG L, et al . NVM-1 predicts prognosis and contributes to growth and metastasis in hepatocellular carcinoma[J ] . Am J Cancer Res, 2017, 7(3): 554-564.

WANG T H, CHAN C W, FANG J Y, et al . 2-O-Methyl-magnolol upregulates the long non-coding RNA, GAS5, and enhances apoptosis in skin cancer cells[J ] . Cell Death Dis, 2017, 8(3): e2638. DOI:10.1038/cddis.2017.66.

DING C, CHENG S, YANG Z, et al . Long non-coding RNA HOTAIR promotes cell migration and invasion via down-regulation of RNA binding motif protein 38 in hepatocellular carcinoma cells[J ] . Int J Mol Sci, 2014, 15(3): 4060-4076. DOI:10.3390/ijms15034060..

刘驰, 杨启, 秦长岭.微血管侵犯对早期肝癌肝切除术切缘选择及患者预后的影响[J].肝胆外科杂志, 2017, 25(5): 353-357.

LIU C, YANG Q, QIN C L. The effect of microvascular invasion on the choice of resection margin of hepatectomy for early stage cancer and the prognosis ofpatients[J]. J Hepatobil Surg, 2017, 25(5) : 353-357.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of SIRT2 knock-down on migration and invasion in hepatocellular carcinoma cells

Effects of LncRNA FGD5-AS1 on migration and invasion of gastric cancer cells by regulating miR-302b-3p/E2F1 axis

Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation, migration and invasion

Mechanism of CPNE3 regulating proliferation, migration and invasion of non-small cell lung cancer cells

Role of E3 ubiquitin ligase MARCH5 in proliferation, invasion and migration of cervical cancer cells

Related Author

宋春丽

任吉华

冉龙宽

李宛蔚

陈娟

LI Na

LI Hao

LIN Kun

Related Institution

Endoscopy Center, Qingdao Central Hospital of Rehabilitation University

Department of Gastroenterology, Qingdao Central Hospital of Rehabilitation University

School of Medicine， Shihezi University

Department of Stomatology， the First Affiliated Hospital of Shihezi University

Department of Pulmonary and Critical Care Medicine, Institute of Respiratory Diseases, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Provmce

⁰