Angptl2 mediates intraocular inflammation by activating Müller cells through PirB

Yao HUANG; Wei FAN; Mei YANG; Rongdi YUAN

doi:10.16016/j.1000-5404.201907167

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Angptl2 mediates intraocular inflammation by activating Müller cells through PirB

Basic Medicine | 更新时间：2026-01-09

- Angptl2 mediates intraocular inflammation by activating Müller cells through PirB
- Journal of Third Military Medical University Vol. 42, Issue 1, Pages: 39-44(2020)
- 作者机构：
  
  400037 重庆，陆军军医大学(第三军医大学)第二附属医院眼科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81470630)
- DOI：10.16016/j.1000-5404.201907167
  CLC： R322.91;R363.21;R364.5
- Received：15 July 2019，
  
  Revised：2019-08-28，
  
  Published：15 January 2020
- 稿件说明：
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Yao HUANG, Wei FAN, Mei YANG, et al. Angptl2 mediates intraocular inflammation by activating Müller cells through PirB[J]. Journal of Third Military Medical University, 2020, 42(1): 39-44.
DOI：

Yao HUANG, Wei FAN, Mei YANG, et al. Angptl2 mediates intraocular inflammation by activating Müller cells through PirB[J]. Journal of Third Military Medical University, 2020, 42(1): 39-44. DOI： 10.16016/j.1000-5404.201907167.

摘要

目的

探讨血管生成素样蛋白2(angiopoietin-like protein 2

Angptl2)在眼内促进炎症的作用和机制。

方法

实验组大鼠玻璃体腔注射0.2 mg LPS，对照组加入等量PBS，RT-PCR检测两组大鼠视网膜Angptl2的表达。以原代培养的SD大鼠视网膜Müller细胞为研究对象，加入外源性Angptl2后，细胞计数观察Müller细胞增殖变化，免疫荧光观察Müller细胞GFAP的表达以及RT-PCR检测促炎因子IL-1β、IL-6、TNF-α的表达变化。免疫荧光观察配对免疫球蛋白样受体B(paired immunoglobulin-like receptorB，PirB)在Müller细胞上的表达；Müller细胞外源性加入Angptl2后，再分别转染Ad-PirB-siRNA腺病毒载体和腺病毒对照Ad-C，分为PBS对照组、Angptl2组、Ad-C+Angptl2组、Ad-PirB-siRNA+Angptl2组，RT-PCR观察炎症因子的表达变化。

结果

LPS组Angptl2 mRNA表达较对照组明显增加(

0.05)；Angptl2组较PBS对照组Müller细胞增殖增多，GFAP表达增加，IL-1β、IL-6、TNF-α表达也增加，差异具有统计学意义(

0.05)；Müller细胞表达Angptl2的配体PirB，Ad-PirB-siRNA干扰Müller细胞PirB表达后，Ad-PirB-siRNA+Angptl2组较Angptl2组和Ad-C+Angptl2组炎症因子IL-6、TNF-α降低

差异具有统计学意义(

0.05)。

结论

Angptl2通过PirB激活Müller细胞分泌促炎因子IL-1β、IL-6、TNF-α参与眼内炎症。

Abstract

Objective

To investigate the pro-inflammatory effects and mechanisms of angiopoietin-like protein 2 (Angptl2) in the eyes.

Methods

SD rats (8 weeks old) were given a single intravitreal injection of 0.2 mg LPS or PBS to establish intraocular inflammation model or sham operation. Then the Angptl2 expression in the retina was examined by real-time PCR (RT-PCR). Müller cells were isolated from the retina of newborn SD rats (with 24 h after born). After the obtained cells were purified and identified

the recombinant Angptl2 was added to the primarily cultured cells. Cell count

immunofluorescence assay and RT-PCR were used to assess the proliferation of Müller cells

the expression of GFAP

and levels of interleukin-1 beta (IL-1β)

interleukin-6 (IL-6) and tumor necrosis factor (TNF-α)

respectively. The expression of Angptl2 ligand

paired immunoglobulin-like receptor B (PirB)

in the Müller cells was observed by immunofluorescence assay. RT-PCR was employed to observed the expression of IL-6 and TNF-α in the Müller cells of PBS group

Angptl2 group

Ad-C (transfection of adenovirus negative control Ad-C)+Angptl2 group

and Ad-PirB-siRNA (transfection of adenovirus Ad-PirB-siRNA)+Angptl2 group.

Results

The mRNA level of Angptl2 was increased notably in the retina of the LPS-treated rats (intraocular inflammation model) than the control rats (

0.05). Exogenous Angptl2 treatment resulted in higher proliferation rate

expression of GFAP and the levels of IL-1β

IL-6 and TNF-α in Müller cells (

0.05). PirB was expressed in the retinal Müller cells. Interference of PirB by adenovirus Ad-PirB-siRNA significantly decreased the levels of IL-6 and TNF-α (

0.05).

Conclusion

Angptl2 mediates acute intraocular inflammation by activating Müller cells to secrets IL-1β

IL-6 and TNF-α though PirB.

关键词

Keywords

references

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