Effects of desflurane on central and peripheral clock genes in mice

Hongni TIAN; Erquan ZHANG; Shengqing LYU; He HUANG; Tiande YANG

doi:10.16016/j.1000-5404.201907166

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Effects of desflurane on central and peripheral clock genes in mice

更新时间：2026-01-09

- Effects of desflurane on central and peripheral clock genes in mice
- Journal of Third Military Medical University Vol. 41, Issue 23, Pages: 2328-2334(2019)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81370210)
- DOI：10.16016/j.1000-5404.201907166
  CLC：
- Received：20 July 2019，
  
  Published：25 December 2019
- 稿件说明：
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Hongni TIAN, Erquan ZHANG, Shengqing LYU, et al. Effects of desflurane on central and peripheral clock genes in mice[J]. Journal of Third Military Medical University, 2019, 41(23): 2328-2334.
DOI：

Hongni TIAN, Erquan ZHANG, Shengqing LYU, et al. Effects of desflurane on central and peripheral clock genes in mice[J]. Journal of Third Military Medical University, 2019, 41(23): 2328-2334. DOI： 10.16016/j.1000-5404.201907166.

摘要

Abstract

Objective

To investigate the effect and mechanism of the anesthetic drug

desflurane

on central and peripheral clock genes in mice.

Methods

C57BL/6J mice were anesthetized with different concentrations (3%

9%) of desflurane

and then the effective concentration of desflurane was determined by detecting the loss of righting reflex. Then C57BL/6J mice and

Per1

-/-

Per

-/-

mice were respectively divided into air control group and desflurane treatment group. The mice from the latter group were given 7% desflurane treatment in 2 h after the light of feeding environment was turned on

and those of the control group was exposed to air at the same time. In 2 h later

the clock genes

Per1

Per2

Dbp

Bmal1

Clock

and

Cry1

in the hypothalamus

kidney and liver tissues were detected by RT-qPCR.

Results

① Treatment of 3% desflurane resulted in no disappearance of righting reflex

while those of 4%

7% and 9% caused the mice achieving loss of the righting reflex accounted for 16.7%

50%

100 % and 100%. Therefore

the effective concentration of desflurane was determined as 7%. ② Compared with the control group

treatment with 7% desflurane significantly decreased the expression of clock gene

Per2

in the hypothalamus tissue in C57BL/6J mice (

0.01)

but had no effect on the expression of the other

5 clock genes (

0.05); It also significantly increased the expression of the clock gene

Per1

while significantly decreased the expression of the clock genes

Bmal1

and

Dbp

in the kidney tissue (

0.05)

but there was no significant change in the expression of the other 3 clock genes (

0.05); In the liver tissue

the expression of clock gene

Per1

was significantly increased (

0.01)

while no changes were seen in the expression of the other 5 clock genes (

0.05). Compare with the control group

the

Per1

-/-

Per2

-/-

mice had significantly decreased the expression of

Bmal1

Clock

and

Dbp

in the hypothalamus tissue after treatment with 7% desflurane (

0.05); obviously reduced expression of

Cry1

and

Clock

(

0.05)

but no notable change in that of

Bmal1

(

0.05) in the liver and kidney tissue.

Conclusion

Desflurane significantly affects the circadian clock genes in mouse central and peripheral tissues

and there is a pathway independent form

Per

gene.

关键词

Keywords

references

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Related Author

LI Mindian

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Related Institution

Department of Cardiovascular Diseases， Center for Circadian Metabolism and Cardiovascular Disease， Key Laboratory of Geriatric Cardiovascular and Cerebrovascular Diseases of Ministry of Education， Southwest Hospital， Army Medical University （Third Military Medical University）

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