Mechanism of exosomes released from bone marrow stem cells regulating proliferation of cardiac microvascular endothelial cells

Chaofu LI; Yan WANG; Ranzun ZHAO; Xianping LONG; Wei ZHANG; Panke CHEN; Bei SHI

doi:10.16016/j.1000-5404.201907063

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Mechanism of exosomes released from bone marrow stem cells regulating proliferation of cardiac microvascular endothelial cells

更新时间：2026-01-09

- Mechanism of exosomes released from bone marrow stem cells regulating proliferation of cardiac microvascular endothelial cells
- Journal of Third Military Medical University Vol. 41, Issue 23, Pages: 2313-2321(2019)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Regional Program of Natural Science Foundation of China(81760042);the Regional Program of Natural Science Foundation of China(81860061);the Project of Science and Technology Plan of Guizhou Province(黔科合LH字7107号)
- DOI：10.16016/j.1000-5404.201907063
  CLC：
- Received：08 July 2019，
  
  Published：25 December 2019
- 稿件说明：
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Chaofu LI, Yan WANG, Ranzun ZHAO, et al. Mechanism of exosomes released from bone marrow stem cells regulating proliferation of cardiac microvascular endothelial cells[J]. Journal of Third Military Medical University, 2019, 41(23): 2313-2321.
DOI：

Chaofu LI, Yan WANG, Ranzun ZHAO, et al. Mechanism of exosomes released from bone marrow stem cells regulating proliferation of cardiac microvascular endothelial cells[J]. Journal of Third Military Medical University, 2019, 41(23): 2313-2321. DOI： 10.16016/j.1000-5404.201907063.

摘要

Abstract

Objective

To investigate the mechanism of exosomes released from bone marrow stem cells (BMSCs) on the proliferation in cardiac microvascular endothelial cells (CMVECs) by miR-214 regulation.

Methods

Rat CMVECs were cultured by enzyme digestion combined with differential adherence. Ultra-high speed centrifugation was used to extract exosomes from normal-oxygen or hypoxic-pretreated BMSCs. Immunofluorescence assay was employed to track the internalization of exosomes in CMVECs. Then the BMSCs were divided into 4 groups: ①control group

②free exosomes (Free-Exos)

③normal exosomes (Nor-Exos)

and ④hypoxia exosomes (Hypoxia-Exos

400 μg/μL exosome co-cultured with CMVECs for 24 h). The cell proliferation was measured by Edu assay and flow cytometry. The miR-214 levels in exosomes or CMVECs were assessed using qRT-PCR

and the levels were measured again after miR-214 in exosomes were inhibited. And the cell proliferation was observed again by the above methods. Finally

after liposome 2000 was employed to transfect miR-214 mimics into CMVECs

the cell proliferation ability was measured by the Edu assay.

Results

Transmission electron microscopy and immunoblotting indicated that the extracted vesicles were exosomes. Immunofluorescence assay revealed that DiI-labeled exosomes could be internalized by CMVECs. Edu assay and cell cycle assay showed that Hypoxic-Exos had stronger potential to promote cell proliferation than Nor-Exos in CMVECs. qRT-PCR results showed that miR-214 was highly expressed in Hypoxic-Exos compared with Nor-Exos (

0.05). In CMVECs

the expression of miR-214 in Hypoxia-Exos group was significantly higher than that in Control group and Nor-Exos group (

0.05). In addition

Hypoxia-Exos's ability to promote cell proliferation was partially blocked by down-regulation of miR-214 in Hypoxia-Exos. However

after overexpression of mir-214 in CMVECs

cell proliferation ability was enhanced.

Conclusion

Exosomes released from hypoxic-pretreated BMSCs can promote CMVECs proliferation by medicating miR-214.

关键词

Keywords

references

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