Proline-rich protein 11 is highly expressed in colorectal cancer to promote cancer cell invasion and metastasis

Yao TANG; Debing XIANG; Jie WANG; Guiyin SUN

doi:10.16016/j.1000-5404.201907034

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Proline-rich protein 11 is highly expressed in colorectal cancer to promote cancer cell invasion and metastasis

Basic Medicine | 更新时间：2026-01-09

- Proline-rich protein 11 is highly expressed in colorectal cancer to promote cancer cell invasion and metastasis
- Journal of Third Military Medical University Vol. 41, Issue 22, Pages: 2181-2189(2019)
- 作者机构：
  
  1.606000 四川泸州，西南医科大学附属医院肿瘤科
  2.402260 重庆，重庆市江津区中心医院肿瘤科
- 作者简介：
- 基金信息：
  
  the Scientific Research Project of Chongqing Municipal Health and Family Planning Commission(2012-1-106)
- DOI：10.16016/j.1000-5404.201907034
  CLC： R341;R730.23;R735.35
- Received：14 July 2019，
  
  Revised：2019-09-11，
  
  Published：30 November 2019
- 稿件说明：
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Yao TANG, Debing XIANG, Jie WANG, et al. Proline-rich protein 11 is highly expressed in colorectal cancer to promote cancer cell invasion and metastasis[J]. Journal of Third Military Medical University, 2019, 41(22): 2181-2189.
DOI：

Yao TANG, Debing XIANG, Jie WANG, et al. Proline-rich protein 11 is highly expressed in colorectal cancer to promote cancer cell invasion and metastasis[J]. Journal of Third Military Medical University, 2019, 41(22): 2181-2189. DOI： 10.16016/j.1000-5404.201907034.

摘要

目的

检测富含脯氨酸蛋白11(proline-rich protein 11，PRR11)在结直肠癌中的表达，探讨其对结直肠癌侵袭和转移的影响。

方法

应用免疫组织化学检测2015年1月至2018年3月重庆市江津区中心医院136例结直肠癌组织与68例癌旁组织中PRR11的表达；Western blot、RT-PCR及免疫荧光检测PRR11及EMT相关信号分子在结直肠癌细胞中的表达；细胞划痕实验、Transwell小室法检测细胞的迁移、侵袭能力。

结果

结直肠癌组织中PRR11阳性表达率高于癌旁组织(60.29%

1.47%

0.01)，且与结直肠癌的远处转移、瘤体大小、TNM分期有关(

0.05)；相比于正常结直肠上皮细胞，PRR11在结直肠癌细胞中高表达(

0.05)；过表达PRR11后，癌细胞HT29的迁移和侵袭能力增强，同时伴随明显的β-catenin核转位，且显著增强β-catenin和Vimentin的表达，抑制E-cadherin的表达(

0.05)；相反，沉默PRR11后，HT29的迁移和侵袭能力减弱，且显著抑制β-catenin和Vimentin的表达，增强E-cadherin的表达(

0.05)。

结论

PRR11在结直肠癌中高表达，且可能通过激活Wnt/β-catenin信号通路，促进β-catenin核转位，从而促进结直肠癌的侵袭和转移。

Abstract

Objective

To investigate the expression of proline-rich protein 11 (PRR11) in colorectal cancer and its role in regulating the invasion and metastasis of colorectal cancer.

Methods

The expression of PRR11 was detected using immunohistochemical SP method in 136 specimens of colorectal cancer tissues and 68 adjacent tissues collected from Jiangjin District Central Hospital between January

2015 and March

2018. We also examined the expression of PRR11 and epithelial-mesenchymal transition (EMT)-related signal molecules in 3 colorectal cancer cell lines (SW480

HT29 and HCT116) and normal human rectal epithelial cells (HCoEpiC) using Western blotting

RT-PCR and immunofluorescence assay; cell scratch assay and Transwell assay were used to compare the migration and invasion abilities of the colorectal cancer cells.

Results

The positive expression rate of PRR11 was significantly higher in colorectal cancer tissue than in the adjacent tissues (60.29%

1.47%

0.01). PRR11 expression was significantly correlated with tumor differentiation

distant metastasis

tumor size and tumor staging (

0.05). Compared with normal colorectal epithelial cells

the colorectal cancer cell lines expressed high levels of PRR11. Overexpression of PRR11 in HT29 cells significantly enhanced the cell migration and invasion ability

caused obvious β-catenin nuclear translocation

significantly up-regulated the expression of β-catenin and vimentin

and decreased the expression of E-cadherin (

0.05). Silencing PRR11 significantly lowered the migration and invasion ability of HT29 cells

decreased the expression of β-catenin and vimentin

and increased the expression of E-cadherin (

0.05).

Conclusion

PRR11 is highly expressed in colorectal cancer to promote tumor invasion and metastasis possibly by promoting the nuclear translocation of β-catenin

via

activating the Wnt/β-catenin signaling pathway.

关键词

Keywords

references

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