Evaluation of human <italic style="font-style: italic">in vitro</italic> skin organ culture in a quasi-static planar homogeneous flow field

Hongtao YAN; Hui TANG; Weiming QIU; Linjuan SONG; Guihong YANG; Jinjin WU

doi:10.16016/j.1000-5404.201907031

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Evaluation of human in vitro skin organ culture in a quasi-static planar homogeneous flow field

更新时间：2026-01-09

- Evaluation of human in vitro skin organ culture in a quasi-static planar homogeneous flow field
- Journal of Third Military Medical University Vol. 41, Issue 23, Pages: 2335-2340(2019)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Major Special Project of Logistics Research of PLA(AWSI4C003-4);the General Program of National Natural Science Foundation of China(31600776)
- DOI：10.16016/j.1000-5404.201907031
  CLC：
- Received：04 July 2019，
  
  Published：25 December 2019
- 稿件说明：
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Hongtao YAN, Hui TANG, Weiming QIU, et al. Evaluation of human in vitro skin organ culture in a quasi-static planar homogeneous flow field[J]. Journal of Third Military Medical University, 2019, 41(23): 2335-2340.
DOI：

Hongtao YAN, Hui TANG, Weiming QIU, et al. Evaluation of human in vitro skin organ culture in a quasi-static planar homogeneous flow field[J]. Journal of Third Military Medical University, 2019, 41(23): 2335-2340. DOI： 10.16016/j.1000-5404.201907031.

摘要

Abstract

Objective

To observe and evaluate the effect of different perfusion frequencies on the survival of human skin specimens in a quasi-static planar homogeneous flow field.

Methods

The foreskin skin grafts in size of 4 mm were placed a quasi-static planar homogeneous flow field

and randomly divided into experimental group (perfused with high frequency) and the control group (perfused with low frequency). After 12 days' culture

PAS staining and VG staining were used to observe the changes of basement membrane

cell morphology and dermal collagen fibers. Moreover

the number of total cells and the apoptotic cells in the 2 groups were separately determined by HE staining and TUNEL assay. The dissolved oxygen concentration in culture medium was measured by a dissolved oxygen meter.

Results

In the low freq

uency perfusion group

gross observation showed characteristic features of necrosis; PAS and VG staining results indicated that the epidermis was severely separated

basement membrane structure disappeared and collagen fibers accumulated. Significant differences were seen in total cell number (249.35±13.00

37.50±9.18

0.001) and apoptotic rate between the experimental group and the control group. The dissolved oxygen level was higher in the former group than the latter group.

Conclusion

In the quasi-static planar homogeneous flow field

high frequency perfusion is suitable for the culture of free skin grafts

in vitro

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references

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Evaluation of human in vitro skin organ culture in a quasi-static planar homogeneous flow field

DOI：10.16016/j.1000-5404.201907031

摘要

Abstract

关键词

Keywords

references