Biological characteristics and preliminary genome analysis of <italic style="font-style: italic">Acinetobacter baumannii</italic> bacteriophage SWH-Ab-3

Ran ZHANG; Juan LUO; Liu WU; Zhulan YANG; Xi LUO; Bo ZHANG

doi:10.16016/j.1000-5404.201902048

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Biological characteristics and preliminary genome analysis of Acinetobacter baumannii bacteriophage SWH-Ab-3

Basic Medicine | 更新时间：2026-01-09

- Biological characteristics and preliminary genome analysis of Acinetobacter baumannii bacteriophage SWH-Ab-3
- Journal of Third Military Medical University Vol. 41, Issue 13, Pages: 1192-1199(2019)
- 作者机构：
  
  1.330031 南昌，南昌大学医学院玛丽女王学院中英2014级6班
  2.400038 重庆，陆军军医大学(第三军医大学)第一附属医院感染管理科
- 作者简介：
- 基金信息：
  
  the Project of Technology Innovation of Social People's Livelihood of Chongqing(cstc2015shmszx120110);the Project of Medical Technology Innovation Plan in Major Clinical Program of Army and Rocket Force(SWH2017ZDCX2009)
- DOI：10.16016/j.1000-5404.201902048
  CLC： Q939.48;R378.7;R394.2
- Received：15 February 2019，
  
  Revised：2019-04-07，
  
  Published：15 July 2019
- 稿件说明：
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Ran ZHANG, Juan LUO, Liu WU, et al. Biological characteristics and preliminary genome analysis of Acinetobacter baumannii bacteriophage SWH-Ab-3[J]. Journal of Third Military Medical University, 2019, 41(13): 1192-1199.
DOI：

Ran ZHANG, Juan LUO, Liu WU, et al. Biological characteristics and preliminary genome analysis of Acinetobacter baumannii bacteriophage SWH-Ab-3[J]. Journal of Third Military Medical University, 2019, 41(13): 1192-1199. DOI： 10.16016/j.1000-5404.201902048.

摘要

目的

从医院污水中分离鲍曼不动杆菌裂解性噬菌体，探讨其生物学特性及基因组基本信息。

方法

以临床分离的鲍曼不动杆菌为指示菌，采用双层琼脂平板法分离、纯化噬菌体，观察噬菌斑特征；利用透射电子显微镜观察噬菌体的形态特征；采用点板试验测定噬菌体的裂菌谱，并观察其最佳感染复数(MOI)、一步生长曲线以及对热、酸碱等理化因素的耐受性。提取噬菌体全基因组DNA，采用Illumina Hiseq2000高通量测序，并对其基因组序列进行注释、比对分析。

结果

从污水中成功分离出1株鲍曼不动杆菌的裂解性噬菌体，命名为SWH-Ab-3；噬菌体头部直径约为70 nm，尾长约80 nm，初步判断其属肌尾噬菌体科；该噬菌体裂菌谱较窄(6株/42株)，最佳感染复数为0.1，潜伏期为40 min，爆发期为50 min，裂解量为31.4 PFU/cell，对热和酸碱环境的耐受性较高。SWH-Ab-3基因组长度为41 730 bp，G+C%含量为39.38%，共编码53个ORF(33个已知功能基因，20个假想蛋白)，其序列与Abp1和vB_AbaP_B3的同源性最高(≥94.0%)，但基因组长度、G+C%含量和形态特征差异显著。

结论

SWH-Ab-3为1株新分离的鲍曼不动杆菌裂解性噬菌体，属肌尾噬菌体科，对热、酸碱等理化因素较耐受，可能通过穿孔素和裂解酶组成的“二元裂解系统”发挥裂菌作用，裂解能力较强，但裂菌谱较窄。

Abstract

Objective

To investigate the biological characteristics and basic genomic information of

Acinetobacter baumannii

(

A. baumannii

) lytic bacteriophage isolated from hospital sewage.

Methods

Bacteriophage was isolated and purified with indicator of

A. baumannii

by double-layer agar plate method

and plaque characteristics of the phage were observed. The structure of purified phage was observed with transmission electron microscopy. The host spectrum was determined by point plate test

and optimal multiplicity of infection (MOI)

one-step growth curve

temperature and pH stability of the phage were investigated. The whole genome DNA of phage was extracted and sequenced by Illumina Hiseq2000 high-throughput sequencing technology

and then the genome sequence was annotated and analyzed by alignment.

Results

A strain of lytic bacteriophage against the

A. baumannii

named SWH-Ab-3

was isolated from sewage. The phage had a head of about 70 nm in diameter and a tail of about 80 nm in length. SWH-Ab-3 was identified to belong to

Myoviridae

family. The bacteriophage had a narrower lytic spectrum of

A. baumannii

(6/42)

optimal MOI of 0.1

latent period of 40 min and burst period of 50 min

and the burst size about 31.4 PFU/cell

and could stand the temperature up to 60 ℃ and keep stable titer under acid or alkali environment. Its genome size was 41 730 bp with G+C content of 39.38%. There were 53 ORF encoded

of which 33 were known functional genes and

20 were hypothetical proteins. The BLASTn alignment found that SWH-Ab-3 had the highest homology with Abp1 and vB_AbaP_B3 (

94%)

but the length of the genome

the G+C% content and the morphological characteristics of the phage were significant.

Conclusion

SWH-Ab-3 is a new lytic phage of

A. baumannii

belonging to the

Myoviridae

family. It is tolerant to heat

acid and alkali. It may lyse the host bacteria through "two-element lysis system"

composed of holin and lyase. It has stronger lytic ability

but narrower lytic spectrum.

关键词

Keywords

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Biological characteristics and preliminary genome analysis of Acinetobacter baumannii bacteriophage SWH-Ab-3

DOI：10.16016/j.1000-5404.201902048

摘要

Abstract

关键词

Keywords

references