Expression of LAPTM4B in glioma tissues and its effect on cell proliferation and invasion

Gang ZHAO; Zheying ZHANG; Baoshun DU; Yang WANG; Huan MA

doi:10.16016/j.1000-5404.201902004

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Expression of LAPTM4B in glioma tissues and its effect on cell proliferation and invasion

更新时间：2026-01-09

- Expression of LAPTM4B in glioma tissues and its effect on cell proliferation and invasion
- Journal of Third Military Medical University Vol. 41, Issue 15, Pages: 1431-1437(2019)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Tackling Project of Science and Technology Research of Henan Province(192102310362)
- DOI：10.16016/j.1000-5404.201902004
  CLC：
- Received：02 February 2019，
  
  Published：15 August 2019
- 稿件说明：
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Gang ZHAO, Zheying ZHANG, Baoshun DU, et al. Expression of LAPTM4B in glioma tissues and its effect on cell proliferation and invasion[J]. Journal of Third Military Medical University, 2019, 41(15): 1431-1437.
DOI：

Gang ZHAO, Zheying ZHANG, Baoshun DU, et al. Expression of LAPTM4B in glioma tissues and its effect on cell proliferation and invasion[J]. Journal of Third Military Medical University, 2019, 41(15): 1431-1437. DOI： 10.16016/j.1000-5404.201902004.

摘要

Abstract

Objective

To detect the protein expression of lysosome-associated protein transmembrane-4 beta (LAPTM4B) in glioma tissues

and to determine its effect on cell proliferation and invasiveness and its correlation with prognosis.

Methods

A total of 86 glioma patients who underwent surgical resection in our hospital from June 2011 to June 2015 were recruited in this study

and they were followed up till June 30

2018. In the same period

25 cases of normal brain tissues were selected. Immunohistochemical assay (SP) was used to detect LAPTM4B protein expression in gliomas and normal brain tissues. Kaplan-Meier analysis and Log-Rank test were performed for survival analysis. Cox proportional hazards model analysis was carried out for risk factors affecting prognosis. U251 cells were cultured and divided into siRNA-LAPTM4B group

siRNA-NC group and blank group. The expression of LAPTM4B in the cells was detected by real-time fluorescent quantitative PCR. Cell proliferation was detected by MTT assay and colony formation assay. Cell migration and invasion was detected by Transwell assay. The protein levels of LAPTM4B

E-cadherin

N-cadherin and Vimentin were detected by Western blotting.

Results

The positive rate of LAPTM4B protein was 77.91% in glioma tissues

which was higher by 28.00% than that in the normal brain tissues (

0.001). The positive expression rate of LAPTM4B protein had statistical difference with different WHO grades (

0.05). Kaplan-Meier survival analysis showed that the median survival time and cumulative survival rate were significantly lower in the LAPTM4B-positive group than the negative group (

=0.002). Cox proportional hazard model analysis indicated that WHO classification and LAPTM4B protein expression were risk factors for prognosis of glioma patients (

0.05). Compared with the siRNA-NC group and the blank group

the relative expression of LAPTM4B at mRNA and protein levels were decreased in the siRNA-LAPTM4B group (

0.05)

and the cell proliferation at 24

72 and 96 h

the numbers of cellular clone

migrated cells and invasive cells

and protein levels of N-cadherin and Vimentin were decreased

while the level of E-cadherin protein was increased in the siRNA-LAPTM4B group (all

0.05).

Conclusion

LAPTM4B protein is highly expressed in glioma tissues

and promotes cell proliferation and invasion

which may be associated with epithelial-mesenchymal transition. Its expression level is correlated with prognosis of the patients.

关键词

Keywords

references

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