Advanced glycation end products enhance invasion and migration of colon cancer HCT116 cells in hypoxic microenvironment

Lin WAN; Yong CHENG; Wuyi WANG; Shuzi LONG; Hongliang LIU; Li ZHOU

doi:10.16016/j.1000-5404.201901095

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Advanced glycation end products enhance invasion and migration of colon cancer HCT116 cells in hypoxic microenvironment

更新时间：2026-01-09

- Advanced glycation end products enhance invasion and migration of colon cancer HCT116 cells in hypoxic microenvironment
- Journal of Third Military Medical University Vol. 41, Issue 15, Pages: 1453-1460(2019)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201901095
  CLC：
- Received：11 January 2019，
  
  Published：15 August 2019
- 稿件说明：
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Lin WAN, Yong CHENG, Wuyi WANG, et al. Advanced glycation end products enhance invasion and migration of colon cancer HCT116 cells in hypoxic microenvironment[J]. Journal of Third Military Medical University, 2019, 41(15): 1453-1460.
DOI：

Lin WAN, Yong CHENG, Wuyi WANG, et al. Advanced glycation end products enhance invasion and migration of colon cancer HCT116 cells in hypoxic microenvironment[J]. Journal of Third Military Medical University, 2019, 41(15): 1453-1460. DOI： 10.16016/j.1000-5404.201901095.

摘要

Abstract

Objective

To investigate the effects of advanced glycation end products (AGEs) on the proliferation

invasion

migration and epithelial-mesenchymal transition (EMT) of colon cancer HCT116 cells in hypoxic microenvironment.

Methods

The optimal AGEs concentration (200 μg/mL) and treatment time (48 h) fo

r promoting the proliferation of HCT116 cells were determined through a screening test with CCK-8 assay and used in subsequent experiment. HCT116 cells were treated with AGEs

hypoxia

or their combination

and the cell cycle changes were detected using flow cytometry; the changes in the invasive and migration abilities of the cells

in vitro

were assessed using Transwell chamber invasion and migration assays. The expressions of HIF-1α

RAGE

E-cadherin

N-cadherin

vimentin and MMP2 proteins in the treated cells were determined with Western blotting.

Results

Compared with the blank control cells

HCT116 cells exposed to a hypoxic microenvironment showed an increased proliferative activity

but AGEs alone did not significantly affect the cell proliferation. The cells treated with AGEs

hypoxia

and their combination all exhibited enhanced invasion and migration abilities compared with the blank control cells. The results of Western blotting showed that the expression level of HIF-1α

which was low in HCT116 cells in normoxic condition

was increased significantly in a hypoxic condition

and increased further after exposure of the cells to both AGEs and hypoxia. The expression of RAGE was increased after AGEs exposure of the cells in both normal and hypoxic environments

and the increase was more prominent in a hypoxic condition. Exposure to AGEs

hypoxia

and both all resulted in increased expressions of MMP2

N-cadherin and vimentin but lowered expression of E-cadherin in the cells.

Conclusion

AGEs can further enhance the invasive and migration abilities but has little effect on the proliferation of HCT116 cells exposed to a hypoxic environment.

关键词

Keywords

references

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