Effect of inhaled nitric oxide on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice

Wenting SU; Yu CUI; Xiaoxu LI; Libin GUAN; Jian HUANG; Yi SHEN

doi:10.16016/j.1000-5404.201812238

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Effect of inhaled nitric oxide on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice

Basic Medicine | 更新时间：2026-01-09

- Effect of inhaled nitric oxide on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice
- Journal of Third Military Medical University Vol. 41, Issue 12, Pages: 1131-1135(2019)
- 作者机构：
  
  1.400016 重庆，重庆医科大学：基础医学院干细胞与组织工程研究室病理生理学教研室
  2.400038 重庆，陆军军医大学(第三军医大学)高原军事医学系高原生理学与病理学教研室，特殊环境医学教育部重点实验室，全军高原医学重点实验
  3.400016 重庆，重庆医科大学：实验教学管理中心
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81370150);Supported by the General Program of National Natural Science Foundation of China(30973446);the Key Special Program of "Thirteenth Five-year Plan" of Logistic Scientific Research of PLA(BWS17J031);the Major Project of "Twelfth Five-year Plan" for Logistic Scientific Research of PLA(AWS14L005)
- DOI：10.16016/j.1000-5404.201812238
  CLC： R331.141;R364.4;R914.3
- Received：12 December 2018，
  
  Revised：2019-02-12，
  
  Published：30 June 2019
- 稿件说明：
移动端阅览
Wenting SU, Yu CUI, Xiaoxu LI, et al. Effect of inhaled nitric oxide on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice[J]. Journal of Third Military Medical University, 2019, 41(12): 1131-1135.
DOI：

Wenting SU, Yu CUI, Xiaoxu LI, et al. Effect of inhaled nitric oxide on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice[J]. Journal of Third Military Medical University, 2019, 41(12): 1131-1135. DOI： 10.16016/j.1000-5404.201812238.

摘要

目的

研究一氧化氮(nitric oxide

NO)对红细胞携氧特性和小鼠耐缺氧能力的影响及两者的相互关系。

方法

取8周龄C57雄性小鼠外周血，置于氧离曲线测绘仪的氧合池中，通入含有NO的氮气，描绘通入不同浓度NO时的氧离曲线，计算氧亲和力参数P

及希尔系数，探讨NO对小鼠外周血红细胞携氧特性的影响。小鼠按随机数字表法分为常氧组和缺氧组(5%O

)

缺氧组再根据未吸入NO及吸入NO浓度(5、10、20 mL/m

)分为4组。小鼠被麻醉后，将其口鼻部置于自制的密闭玻璃箱。玻璃箱预先通入纯N

、NO、15%O

，将气体浓度预置为5%O

和相应浓度的NO。用血氧饱和度仪检测鼠尾血氧饱和度，同时记录小鼠的存活时间。

结果

通入100、200 mL/m

NO均可使氧离曲线发生明显左移，显著降低P

(

0.05)。与常氧组比较，缺氧小鼠血氧饱和度显著降低，吸入20 mL/m

NO显著削弱小鼠在缺氧环境中血氧饱和度的降低程度(

0.05)。与未吸入NO的缺氧对照组比较，吸入5、10、20 mL/m

NO组小鼠存活时间均延长，其中10、20 mL/m

NO组小鼠存活时间的延长差异具有统计学意义(

0.05)。吸入20 mL/m

NO时，缺氧条件下的存活时间与血氧饱和度呈正相关关系(

0.05)。

结论

吸入NO增加细胞血红蛋白氧亲和力，改善缺氧条件下血氧饱和度；NO对红细胞携氧特性的改善作用与缺氧耐受力增强有关。

Abstract

Objective

To study the effects of nitric oxide (NO) inhalation on oxygen-carrying capacity of red blood cells and hypoxia tolerance in mice.

Methods

Peripheral blood from 8-week-old male mice was exposed to nitrogen gas containing NO in an oxygenation pool to generate the oxygen dissociation curve and calculate the oxygen affinity parameter P

and the Hill coefficient. Fifty 8-week-old male C57 mice were randomized into 5 groups (

=10) and subjected to inhalation of 21% O

5% O

or 5% O

with 5

10 or 20 mL/m

NO under anesthesia. The oxygen saturation in the tail vein blood of the mice was measured with an oximeter following the exposures

and the survival time of the mice was recorded.

Results

The introduction

of 100 and 200 mL/m

NO into the pooled whole blood resulted in a significant left shift of the oxygenation curve

and significantly decreased P

(

0.05) in a concentration-dependent manner. Hypoxic exposure of the mice caused a significant reduction in the oxygen saturation level in the tail vein blood

and this reduction was obviously attenuated by inhalation of 20 mL/m

NO (

0.05). Compared with hypoxia exposure

inhalation of 5

and 20 mL/m

NO concentration-dependently prolonged the survival time of the mice

and the effect was statistically significantly in mice inhaling 10 and 20 mL/m

NO (

0.05). In mice inhaling 20 mL/m

the survival time under hypoxic conditions was positively correlated with the level of blood oxygen saturation (

0.05).

Conclusion

Inhalation of NO increases hemoglobin oxygen affinity and improves blood oxygen saturation in mice exposed to hypoxic conditions. NO enhances hypoxia tolerance by improving the oxygen-carrying capacity of the red blood cells.

关键词

Keywords

references

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Related Author

Yi SHEN

Libin GUAN

Xiaoxu LI

Wenting SU

Yu CUI

Jian HUANG

Ming WEI

Liangming LIU

Related Institution

Laboratory of Stem Cell and Tissue Engineering & Department of Pathophysiology, College of Basic Medical Sciences

Department of High Altitude Physiology and High Altitude Biology, Key Laboratory of High Altitude Medicine of PLA, Key Laboratory of Extreme Environmental Medicine of Ministry Education, Faculty of High Altitude Military Medicine

Institute of Pathology, First Affiliated Hospital, Army Medical University(Third Military Medical University)

Department of Shock and Transfusion, Army Medical Center of PLA

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