Developmental process and expression patterns of fibroblast growth factor receptors in murine meniscus after birth

Fangfang LI; Yangli XIE; Junlan HUANG; Ruobin ZHANG; Wanling JIANG; Dali ZHANG; Meng XU; Xiaolan DU; Lin CHEN

doi:10.16016/j.1000-5404.201812209

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Developmental process and expression patterns of fibroblast growth factor receptors in murine meniscus after birth

Basic Medicine | 更新时间：2026-01-09

- Developmental process and expression patterns of fibroblast growth factor receptors in murine meniscus after birth
- Journal of Third Military Medical University Vol. 41, Issue 7, Pages: 646-652(2019)
- 作者机构：
  
  400042 重庆，陆军军医大学(第三军医大学)大坪医院野战外科研究所战创伤康复医学研究室，全军军事训练伤防治与康复实验室, 创伤、烧伤与复合伤国家重点实验室
- 作者简介：
- 基金信息：
  
  the Key Program of National Natural Science Foundation of China(81830075);the Key Project of Innovation Program in Military Medicine(16CXZ016)
- DOI：10.16016/j.1000-5404.201812209
  CLC： R322.72;R329.24
- Received：26 December 2018，
  
  Revised：2019-02-14，
  
  Published：15 April 2019
- 稿件说明：
移动端阅览
Fangfang LI, Yangli XIE, Junlan HUANG, et al. Developmental process and expression patterns of fibroblast growth factor receptors in murine meniscus after birth[J]. Journal of Third Military Medical University, 2019, 41(7): 646-652.
DOI：

Fangfang LI, Yangli XIE, Junlan HUANG, et al. Developmental process and expression patterns of fibroblast growth factor receptors in murine meniscus after birth[J]. Journal of Third Military Medical University, 2019, 41(7): 646-652. DOI： 10.16016/j.1000-5404.201812209.

摘要

目的

观察小鼠出生后半月板发育过程的组织形态学变化及3种成纤维细胞生长因子受体(fibroblast growth factor receptors，FGFRs)的时空表达规律。

方法

取材出生后不同年龄(1日龄和1、2、4、6、12周龄)C57BL/6J小鼠膝关节行石蜡切片，藏红-固绿染色观察小鼠半月板的结构，用免疫组织化学方法检测小鼠半月板Ⅰ型胶原(type Ⅰ collagen

Col Ⅰ)、Ⅱ型胶原(type Ⅱ collagen

Col Ⅱ)和FGFR1、2、3的表达。

结果

小鼠出生后，半月板具有明确的内-外侧分区，内侧区富含蛋白聚糖，藏红着色较深，而外侧区藏红着色浅。1周时分区更加明显，内侧区主要表达Ⅱ型胶原，而外侧区主要表达Ⅰ型胶原。出生后2周，半月板中间部分细胞开始肥大化

4周时细胞肥大化进一步加重，到6周时，在半月板前角出现小面积的矿化。12周时，半月板出生后的发育过程基本完成，前角出现类似骨髓腔的结构。整个发育过程，FGFR1、2、3的时空表达模式相似，表现为前角的表达于1~2周时达到高峰，而后逐渐减少(

0.05)，并由内侧区均匀分布逐渐变为表层边缘分布，而后角FGFRs的表达无明显变化。

结论

小鼠半月板在出生后的发育过程中，软骨基质、胶原分布以及FGFRs的表达均存在时空特异性规律，提示FGFRs在半月板发育过程中起重要作用。

Abstract

Objective

To observe the developmental process of murine meniscus after birth and investigate the expression patterns of 3 types of fibroblast growth factor receptors (FGFRs) during this process.

Methods

Mice at different ages (1 d old and 1

6 and 12 weeks old) were sacrificed and the knee joints were harvested and embedded in paraffin. The sagittal sections of knee joint were stained with Safranin O-fast green to observe the structure of murine meniscus. The expression of type Ⅰ collagen

type Ⅱ collagen

and 3 types of FGFRs were detected by immunohistochemistry.

Results

At birth

the meniscus exhibited distinguishable inner and outer regions

with proteoglycan of darker staining rich in the inner region

while the outer region lightly stained. At 1-week age

the inner-outer region was more obvious

and the inner region mainly expressed type Ⅱ collagen

while the outer region expressed type Ⅰ collagen. At 2 weeks

some cells in the anterior horn became hypertrophied

and the hypertrophy was further aggravated at 4 weeks of age. By 6 weeks

ossification tissues were observed in the anterior horn of the meniscus. At 12 weeks

the meniscal developmental process after birth was basically completed

and mineralized structure resembling bone marrow cavity was found in the anterior horn. The expression of the 3 types of FGFRs had similar spatiotemporal pattern which reached a peak at 1~2 weeks of age and then gradually decreased (

0.05) with its spatial distribution changing from a uniform distribution to an edged distribution in the inner zone of anterior horn. However

the expression of FGFRs in the posterior horn had no obvious changes.

Conclusion

During the postnatal development of murine meniscus

the cartilage matrix

collagen distribution and expression patterns of 3 types of FGFRs have spatiotemporal specificity

which suggest that FGFRs play an important role in the meniscal development.

关键词

Keywords

references

MAKRIS E A, HADIDI P, ATHANASIOU K A. The knee meniscus: structure-function, pathophysiology, current repair techniques, and prospects for regeneration[J]. Biomaterials, 2011, 32(30): 7411-7431. DOI:10.1016/j.biomaterials.2011.06.037.

MCDEVITT C A, WEBBER R J. The ultrastructure and biochemistry of meniscal cartilage[J]. Clin Orthop Relat Res, 1990(252): 8-18. DOI:10.1097/00003086-19900 3000-00003.

MCNULTY A L, GUILAK F. Mechanobiology of the meniscus[J]. J Biomech, 2015, 48(8): 1469-1478. DOI:10.1016/j.jbiomech.2015.02.008.

ARNOCZKY S P, WARREN R F. Microvasculature of the human meniscus[J]. Am J Sports Med, 1982, 10(2): 90-95. DOI:10.1177/036354658201000205.

ANDERSSON-MOLINA H, KARLSSON H, ROCKBORN P. Arthroscopic partial and total meniscectomy[J]. Arthroscopy, 2002, 18(2): 183-189. DOI:10.1053/jars.2002.30435.

ROOS E M, OSTENBERG A, ROOS H, et al. Long-term outcome of meniscectomy: symptoms, function, and performance tests in patients with or without radiographic osteoarthritis compared to matched controls[J]. Osteoarthr Cartil, 2001, 9(4): 316-324. DOI:10.1053/joca.2000.0391.

SIHVONEN R, PAAVOLA M, MALMIVAARA A, et al. Arthroscopic partial meniscectomy versus sham surgery for a degenerative meniscal tear[J]. N Engl J Med, 2013, 369(26): 2515-2524. DOI:10.1056/NEJMoa1305189.

SANCHEZ-ADAMS J, ATHANASIOU K A. The knee meniscus: a complex tissue of diverse cells[J]. Cel Mol Bioeng, 2009, 2(3): 332-340. DOI:10.1007/s12195-009-0066-6.

SU N, JIN M, CHEN L. Role of FGF/FGFR signaling in skeletal development and homeostasis: learning from mouse models[J]. Bone Res, 2014, 2: 14003. DOI:10.1038/boneres.2014.3.

ORNITZ D M, MARIE P J. Fibroblast growth factor signaling in skeletal development and disease[J]. Genes Dev, 2015, 29(14): 1463-1486. DOI:10.1101/gad.266551.115.

VERDONK P C M, FORSYTH R G, WANG J, et al. Characterisation of human knee meniscus cell phenotype[J]. Osteoarthr Cartil, 2005, 13(7): 548-560. DOI:10.1016/j.joca.2005.01.010.

MELROSE J, SMITH S, CAKE M, et al. Comparative spatial and temporal localisation of perlecan, aggrecan and type Ⅰ, Ⅱ and Ⅳ collagen in the ovine meniscus: an ageing study[J]. Histochem Cell Biol, 2005, 124(3/4): 225-235. DOI:10.1007/s00418-005-0005-0.

FOX A J, BEDI A, RODEO S A. The basic science of human knee menisci: structure, composition, and function[J]. Sports Health, 2012, 4(4): 340-351. DOI:10.1177/1941738111429419.

KREINEST M, REISIG G, STRÖBEL P, et al. Analysis of gene expression and ultrastructure of stifle menisci from juvenile and adult pigs[J]. Comp Med, 2016, 66(1): 30-40.

GLASS R S, BARNES W M, KELLS D U, et al. Ossicles of knee menisci[J]. Clin Orthop Relat Res, 1975, 111: 163-171. DOI:10.1097/00003086-197509000-00023.

ISEKI S, WILKIE A O, MORRISS-KAY G M. Fgfr1 and Fgfr2 have distinct differentiation- and proliferation-related roles in the developing mouse skull vault[J]. Development, 1999, 126(24): 5611-5620.

苏楠, 陈林.FGFG3在骨骼发育和疾病中作用的研究进展[J].国际遗传学杂志, 2006, 29(3):222-225. DOI:10.3760/cma.j.issn.1673-4386.2006.03.0157.

SU N, CHEN L. The effect of FGFR3 on skeleton development and disease[J]. Int J Genet, 2006, 29(3):222-225. DOI:10.3760/cma.j.issn.1673-4386.2006.03.0157.

HYDE G, BOOT-HANDFORD R P, WALLIS G A. Col2a1 lineage tracing reveals that the meniscus of the knee joint has a complex cellular origin[J]. J Anat, 2008, 213(5):531-538.DOI:10.1111/j.1469-7580.2008.00966.x.

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