Agrin reduces heterogenous expression of nicotinic acetylcholine receptors in skeletal muscles of septic rats

Bixiao LYU; Su MIN; Jun YANG

doi:10.16016/j.1000-5404.201812109

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Agrin reduces heterogenous expression of nicotinic acetylcholine receptors in skeletal muscles of septic rats

Basic Medicine | 更新时间：2026-01-09

- Agrin reduces heterogenous expression of nicotinic acetylcholine receptors in skeletal muscles of septic rats
- Journal of Third Military Medical University Vol. 41, Issue 12, Pages: 1125-1130, 1135(2019)
- 作者机构：
  
  400016 重庆, 重庆医科大学附属第一医院麻醉科
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81471282)
- DOI：10.16016/j.1000-5404.201812109
  CLC： R337.4;R341;R631
- Received：13 December 2018，
  
  Revised：2019-02-15，
  
  Published：30 June 2019
- 稿件说明：
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Bixiao LYU, Su MIN, Jun YANG. Agrin reduces heterogenous expression of nicotinic acetylcholine receptors in skeletal muscles of septic rats[J]. Journal of Third Military Medical University, 2019, 41(12): 1125-1130, 1135.
DOI：

Bixiao LYU, Su MIN, Jun YANG. Agrin reduces heterogenous expression of nicotinic acetylcholine receptors in skeletal muscles of septic rats[J]. Journal of Third Military Medical University, 2019, 41(12): 1125-1130, 1135. DOI： 10.16016/j.1000-5404.201812109.

摘要

目的

探讨聚集蛋白(agrin)对脓毒症大鼠骨骼肌烟碱型乙酰胆碱受体(nicotinic acetylcholine receptor

nAChR)亚型异化表达的作用。

方法

采用盲肠结扎穿孔(cecal ligation and puncture

CLP)建立脓毒症大鼠模型。48只2月龄健康雄性SD大鼠采用随机数字表法分为假手术组(Sham组)、假手术+agrin组(ShamA组)、脓毒症组(CLP组)和脓毒症+agrin组(CLPA组)，每组12只。假手术组仅暴露腹腔不进行盲肠结扎穿孔手术，ShamA组和CLPA组在建模后胫前肌内注射100 μg/mL agrin溶液0.1 mL。建模后第48小时取大鼠血液样品，检测血浆中炎症因子IL-6水平。行复合肌动作电位(compound muscle action potential

CMAP)检测其振幅、时程、潜伏期及神经传导速度。Western blot和免疫荧光检测大鼠胫前肌组织γ-nAChR和α7-nAChR蛋白表达。

结果

Sham组和ShamA组血浆IL-6水平明显低于CLP组和CLPA组(

0.05)。与Sham组和ShamA组比较，CLP组与CLPA组CMAP时程、潜伏期延长(

0.05)，γ-nAChR和α7-nAChR蛋白表达升高(

0.05)，而CMAP振幅及传导速度降低(

0.05)。与CLP组比较，CLPA组的CMAP时程、潜伏期缩短(

0.05)，γ-nAChR和α7-nAChR蛋白表达降低(

0.05)，而CMAP振幅及传导速度升高(

0.05)。

结论

Agrin可以减轻脓毒症导致的神经肌肉功能障碍并减少异质化烟碱型乙酰胆碱受体的表达。

Abstract

Objective

To explore the effect of agrin on the heterogenous expression of nicotinic acetylcholine receptors (nAChRs) in the skeletal muscles of rats with sepsis.

Methods

Forty-eight 2-month-old healthy SD rats were randomized equally into sham-operated group

sham+agrin group (ShamA group)

cecal ligation and puncture (CLP) group

and CLP+agrin group (CLPA group) and received either CLP to establish models of sepsis or a sham operation of laparotomy without CLP as indicated. In ShamA group and CLPA group

the rats were given an intramuscular injection of 0.1 mL agrin (100 μg/mL) into the anterior tibial muscle after the operation. At 48 h after the modeling

blood samples were collected from the rats for measurement of plasma interleukin-6 (IL-6) level

and the compound muscle action potentials (CMAP) were detected to assess the neuromuscular conduction. The protein expression levels of type γ-nAChR and α7-nAChR in the anterior tibial muscle were detected using Western blotting and immunofluorescence staining.

Results

The plasma IL-6 levels were significantly lower in the sham-operated and ShamA group than in CLP and CLPA groups (

0.05) and were comparable between the latter 2 groups (

0.05). Compared with those in the 2 sham-operated groups

the duration and latency of CMAP were increased significantly while the amplitude and nerve conduction velocity were decreased obviously in CLP group (

0.05). Compared with those in CLP group

the duration and latency of CMAP were decreased and the amplitude and nerve conduction velocity were increased significantly in CLPA group (

0.05). In the anterior tibial muscle

the protein expression levels of γ-nAChR and α7-nAChR were significantly higher in CLP and CLPA groups than in the 2 sham-operated groups (

0.05)

and their levels were the highest in CLP group (

0.05).

Conclusion

Agrin can improve neuromuscular function in rats with sepsis

which is likely attributed to down-regulated heterogeneous expression of γ-nAChR and α7-nAChR in the skeletal muscles.

关键词

Keywords

references

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