Clinical Significance of GCNT3 in colorectal cancer

Shanxi LIU; Jun YE; Yangyang SHANG; Haoyuan CHEN; Rongquan WANG

doi:10.16016/j.1000-5404.201812043

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Clinical Significance of GCNT3 in colorectal cancer

Basic Medicine | 更新时间：2026-01-09

- Clinical Significance of GCNT3 in colorectal cancer
- Journal of Third Military Medical University Vol. 41, Issue 10, Pages: 913-917(2019)
- 作者机构：
  
  重庆，陆军军医大学西南医院全军消化病研究所
- 作者简介：
- 基金信息：
  
  General Program of National Natural Science Foundation of China(81572903)
- DOI：10.16016/j.1000-5404.201812043
  CLC： R574.6;R735.3
- Received：05 December 2018，
  
  Revised：2019-01-28，
  
  Published：30 May 2019
- 稿件说明：
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Shanxi LIU, Jun YE, Yangyang SHANG, et al. Clinical Significance of GCNT3 in colorectal cancer[J]. Journal of Third Military Medical University, 2019, 41(10): 913-917.
DOI：

Shanxi LIU, Jun YE, Yangyang SHANG, et al. Clinical Significance of GCNT3 in colorectal cancer[J]. Journal of Third Military Medical University, 2019, 41(10): 913-917. DOI： 10.16016/j.1000-5404.201812043.

摘要

目的

探讨GCNT3在结直肠癌中表达的水平变化及其对结直肠癌患者预后的影响。

方法

国际癌症和肿瘤基因数据库(TCGA)中下载结直肠癌患者GCNT3基因(编码C2GnT-2)的mRNA表达数据及临床信息，整理分析其表达水平变化对结直肠癌临床相关性及预后的影响，并通过细胞增殖实验观察干扰C2GnT-2合成后结直肠癌细胞增殖能力的变化情况。

结果

GCNT3在癌组织中较癌旁组织低表达(

0.001)；GCNT3低表达组病人无病生存率显著低于高表达组(

0.05)；干扰C2GnT-2合成后结直肠癌细胞增殖能力显著增强(

0.01)。

结论

GCNT3在结直肠癌中低表达，其低表达可能提示直肠癌患者无病生存率低。

Abstract

Objective

To investigate the expression level of GCNT3

a glycosyltransferase enzyme

in colorectal cancer (CRC) and its association with the prognosis of the patients.

Methods

The mRNA expression data of GCNT3 gene (encoding mucin-type core 2 synthase enzyme

C2GnT-2) and clinical information of the patients were obtained from the Cancer Genome Atlas database (TCGA). The correlation of GCNT3 mRNA expression level with the clinical characteristics of the patients was analyzed. Kaplan-Meier analysis and Cox regression analysis were used for assessing the prognostic value of GCNT3. We also tested the effect of a short hairpin RNA (shRNA) targeting GCNT3 on the proliferation of the colorectal cancer cell line HT-29 using MTT assay.

Results

GCNT3 mRNA expression level was significantly decreased in CRC tissues as compared with the paired adjacent colorectal tissues (

0.001). A low expression level of GCNT3 was significantly correlated with a reduced disease-free survival (DFS) rate in the patients with CRC (

0.05). In HT-29 cells

interference of C2GnT-2 with the specific shRNA significantly promoted the cell proliferation as compared with the control cells (

0.01).

Conclusion

The expression of GCNT3 is significantly downregulated in CRC tissues

and a low expression of GCNT3 can be indicative of a low disease-free survival rate of patients with CRC.

关键词

Keywords

references

DEKKER E, REX D K. Advances in CRC prevention: screening and surveillance. Gastroenterology, 2018, 154(7): 1970-1984. DOI: 10.1053/j.gastro.2018.01.069.

KAILEMIA M J, PARK D, LEBRILLA C B. Glycans and glycoproteins as specific biomarkers for cancer. Anal Bioanal Chem, 2017, 409(2): 395-410. DOI: 10.1007/s00216-016-9880-6.

BANSIL R, TURNER B S. The biology of mucus: Composition, synthesis and organization. Adv Drug Deliv Rev, 2018, 124: 3-15. DOI: 10.1016/j.addr.2017.09.023.

HUANG M C, CHEN H Y, HUANG H C, et al . C2GnT-M is downregulated in colorectal cancer and its re-expression causes growth inhibition of colon cancer cells. Oncogene, 2006, 25(23): 3267-3276. DOI: 10.1038/sj.onc.1209350.

RAO C V, JANAKIRAM N B, MOHAMMED A. Molecular pathways: mucins and drug delivery in cancer. Clin Cancer Res, 2017, 23(6): 1373-1378. DOI: 10.1158/1078-0432.CCR-16-0862.

WEINSTEIN J N, COLLISSON E A, MILLS G B, et al . The cancer genome atlas pan-cancer analysis project. Nat Genet, 2013, 45(10): 1113-1120. DOI: 10.1038/ng.2764.

ZHU Y T, QIU P, JI Y. TCGA-assembler: open-source software for retrieving and processing TCGA data. Nat Methods, 2014, 11(6): 599-600. DOI: 10.1038/nmeth.2956.

YE J, SONG L L, LIU Y, et al . Core 2 mucin-type O-glycan is related to EPEC and EHEC O157: H7 adherence to human colon carcinoma HT-29 epithelial cells. Dig Dis Sci, 2015, 60(7): 1977-1990. DOI: 10.1007/s10620-015-3548-5.

CAMP R L, DOLLED-FILHART M, RIMM D L. X-tile: a new bio-informatics tool for biomarker assessment and outcome-based cut-point optimization. Clin Cancer Res, 2004, 10(21): 7252-7259. DOI: 10.1158/1078-0432.CCR-04-0713.

ZHU J Z, TAN Z Q, HOLLIS-HANSEN K, et al . Epidemiological trends in colorectal cancer in China: An ecological study. Dig Dis Sci, 2017, 62(1): 235-243. DOI: 10.1007/s10620-016-4362-4.

LIU T H, ZHANG S, CHEN J, et al . The transcriptional profiling of glycogenes associated with hepatocellular carcinoma metastasis. PLoS one, 2014, 9(9): e107941. DOI: 10.1371/journal.pone.0107941.

GONZÁLEZ-VALLINAS M, MOLINA S, VICENTE G, et al . Expression of microRNA-15b and the glycosyltransferase GCNT3 correlates with antitumor efficacy of Rosemary diterpenes in colon and pancreatic cancer. PLoS one, 2014, 9(6): e98556. DOI:10.1371/journal.pone.0098556.

STONE E L, ISMAIL M N, LEE S H, et al . Glycosyltransferase function in core 2-type protein O glycosylation. Mol Cell Biol, 2009, 29(13): 3770-3782. DOI: 10.1128/MCB.00204-09.

HUNG J S, HUANG J, LIN Y C, et al . C1GALT1 overexpression promotes the invasive behavior of colon cancer cells throu gh modifying O-glycosylation of FGFR2. Oncotarget, 2014, 5(8): 2096-2106. DOI: 10.18632/oncotarget.1815.

BAST R C Jr, HENNESSY B, MILLS G B. The biology of ovarian cancer: new opportunities for translation. Nat Rev Cancer, 2009, 9(6): 415-428. DOI: 10.1038/nrc2644.

MASOUMI MOGHADDAM S, AMINI A, MORRIS D L, et al . Significance of vascular endothelial growth factor in growth and peritoneal dissemination of ovarian cancer. Cancer Metast Rev, 2012, 31(1/2): 143-162. DOI: 10.1007/s10555-011-9337-5.

LI Q, RAN P Z, ZHANG X Y, et al . Downregulation of N-acetylglucosaminyltransferase GCNT3 by mir-302b-3p decreases non-small cell lung cancer (NSCLC) cell proliferation, migration and invasion. Cell Physiol Biochem, 2018, 50(3): 987-1004. DOI: 10.1159/000494482.

YE J, WEI X, SHANG Y, et al . Core 3 mucin-type O-glycan restoration in colorectal cancer cells promotes MUC1/p53/miR-200c-dependent epithelial identity. Oncogene, 2017, 36(46): 6391-6407. DOI: 10.1038/onc.2017.241.

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