Establishment and evaluation of a mouse model of pulmonary <italic style="font-style: italic">Pseudomonas aeruginosa</italic> infection by intratracheal instillation

Yu WANG; Dinglin ZHANG; Wei FENG; Lin CHENG; Qian YUAN; Xiaoyu LI; Fengjun SUN

doi:10.16016/j.1000-5404.201811157

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Establishment and evaluation of a mouse model of pulmonary Pseudomonas aeruginosa infection by intratracheal instillation

Basic Medicine | 更新时间：2026-01-09

- Establishment and evaluation of a mouse model of pulmonary Pseudomonas aeruginosa infection by intratracheal instillation
- Journal of Third Military Medical University Vol. 41, Issue 10, Pages: 918-922(2019)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)：第一附属医院药学部
  2.400038 重庆，陆军军医大学(第三军医大学)：基础部化学教研室
  3.056000 河北邯郸，解放军980医院邯郸分院药剂科
- 作者简介：
- 基金信息：
  
  Program of National Natural Science Foundation for Young Scholars of China(81301397)
- DOI：10.16016/j.1000-5404.201811157
  CLC： R378;R459.7;R563.1
- Received：22 November 2018，
  
  Revised：2019-01-08，
  
  Published：30 May 2019
- 稿件说明：
移动端阅览
Yu WANG, Dinglin ZHANG, Wei FENG, et al. Establishment and evaluation of a mouse model of pulmonary Pseudomonas aeruginosa infection by intratracheal instillation[J]. Journal of Third Military Medical University, 2019, 41(10): 918-922.
DOI：

Yu WANG, Dinglin ZHANG, Wei FENG, et al. Establishment and evaluation of a mouse model of pulmonary Pseudomonas aeruginosa infection by intratracheal instillation[J]. Journal of Third Military Medical University, 2019, 41(10): 918-922. DOI： 10.16016/j.1000-5404.201811157.

摘要

目的

建立小鼠铜绿假单胞菌(Pseudomonas aeruginosa

PA)肺部感染模型，并对该模型进行初步评价。

方法

采用一种快速无创的小鼠气管插管滴注法，将小鼠在透射灯照射下经口咽气管插管，滴注1×10

CFU/mL的铜绿假单胞菌悬液50 μL，待接种感染7 d后处死，进行肺部菌落计数和病理学评价。

结果

经气管接种感染后，与对照组小鼠比较，感染组小鼠生理状态不佳，肺组织匀浆检出PA，血液中白细胞和中性粒细胞数目显著增多(

0.05)，且出现肺部病理学改变，显示有炎症反应；对照组小鼠肺组织匀浆则未见PA生长，且血液中白细胞和中性粒细胞数目正常，未出现炎症反应。

结论

采用小鼠气管插管滴注法，通过呼吸道感染途径可成功建立小鼠肺部PA感染模型，该模型具有稳定性好，易定量的优点。

Abstract

Objective

To establish and validate a mouse model of pulmonary infection of

Pseudomonas aeruginosa

Methods

The models of pulmonary

Pseudomonas aeruginosa

infection was established in mice by intratracheal instillation of 50 μL (1×10

CFU/mL)

Pseudomonas aeruginosa

suspension delivered through orotracheal intubation under a transillumination lamp. At 7 d after the infection

the mice were sacrificed for counting the bacterial colonies in the lungs and for pathological evaluation.

Results

After intratracheal instillation of

Pseudomonas aeruginosa

the mice showed a poor general condition and had positive findings of

Pseudomonas aeruginosa

in the lung tissues with significantly increased blood white blood cell and neutrophil counts compared with the control mice (

0.05); pathological examination revealed obvious pathological changes and inflammations in the lungs of the mice after the infection. In the control mice

no bacteria or path

ological changes were detected in the lung tissue

and the white blood cell and neutrophil counts were normal without evidence of inflammatory responses.

Conclusion

We successfully established a mouse model of pulmonary

Pseudomonas aeruginosa

infection by delivering the pathogens through tracheal intubation. This model shows a good stability and allows convenient quantitative analysis.

关键词

Keywords

references

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Establishment and evaluation of a mouse model of pulmonary Pseudomonas aeruginosa infection by intratracheal instillation

DOI：10.16016/j.1000-5404.201811157

摘要

Abstract

关键词

Keywords

references