Silicone rubber hardness regulates fibroblasts proliferation and expression of α-SMA and CollagenⅠ by autophagy

Hailin WANG; Yang GAO; Xiaohua SHI; Yichen DU; Yiming ZHANG; Dongli FAN

doi:10.16016/j.1000-5404.201811069

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Silicone rubber hardness regulates fibroblasts proliferation and expression of α-SMA and CollagenⅠ by autophagy

Basic Medicine | 更新时间：2026-01-09

- Silicone rubber hardness regulates fibroblasts proliferation and expression of α-SMA and CollagenⅠ by autophagy
- Journal of Third Military Medical University Vol. 41, Issue 9, Pages: 829-835(2019)
- 作者机构：
  
  1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院整形美容外科
  2.401120 重庆，重庆医科大学附属第三医院皮肤整形美容中心
- 作者简介：
- 基金信息：
  
  the Key Program of National Natural Science Foundation of China(81372075);the Sci & Tech Achievements Transformation Fund of Third Military Medical University(2012XZH05)
- DOI：10.16016/j.1000-5404.201811069
  CLC： R318.08;R322.991;R329.28
- Received：10 November 2018，
  
  Revised：2019-01-31，
  
  Published：15 May 2019
- 稿件说明：
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Hailin WANG, Yang GAO, Xiaohua SHI, et al. Silicone rubber hardness regulates fibroblasts proliferation and expression of α-SMA and CollagenⅠ by autophagy[J]. Journal of Third Military Medical University, 2019, 41(9): 829-835.
DOI：

Hailin WANG, Yang GAO, Xiaohua SHI, et al. Silicone rubber hardness regulates fibroblasts proliferation and expression of α-SMA and CollagenⅠ by autophagy[J]. Journal of Third Military Medical University, 2019, 41(9): 829-835. DOI： 10.16016/j.1000-5404.201811069.

摘要

目的

探讨不同硬度硅橡胶对人真皮成纤维细胞增殖及其表达α-SMA和CollagenⅠ的影响，并研究自噬在其中所起的作用。

方法

将A、B组分液体硅胶按不同比例混合固化制成一系列不同硬度的硅橡胶片，测定其邵氏硬度、断裂伸长率、水接触角、表面形貌等机械性能。利用CCK-8检测成纤维细胞在不同硬度硅橡胶表面的增殖情况，而后选取细胞增殖存在显著差异的两种硬度硅橡胶进一步实验，采用Western blot和ELISA法分别检测正常条件下、施加自噬抑制剂3-MA和诱导剂西罗莫司后成纤维细胞自噬标志蛋白和α-SMA、Collagen Ⅰ的表达情况。

结果

成纤维细胞增殖能力随硅橡胶硬度的增加而增加，差异有统计学意义(

0.05)。高硬度硅橡胶上的成纤维细胞具有更高的自噬活性，且α-SMA、Collagen I的表达量比低硬度硅橡胶更低，差异有统计学意义(

0.01)。

结论

硅橡胶硬度可通过自噬调控成纤维细胞的增殖，高硬度硅橡胶上调成纤维细胞自噬活性从而促进成纤维细胞增殖并抑制其表达α-SMA和CollagenⅠ。

Abstract

Objective

To investigate the effect of different hardness of silicone rubber (SRs) on the proliferation and expression of α- SMA and Collagen Ⅰ in human dermal fibroblasts (HFBs)

and study the role of autophagy in the process.

Methods

In this study

we prepared a series of SRs with different hardness by changing the ratio of liquid silica gel component A to B. The mechanical properties of SRs such as A-shore hardness

breaking elongation

water contact angle and surface morphology were measured. CCK-8 assay was used to study the proliferation of HFBs cultivated on the surface of these SRs. Then

2 kinds of SRs with significant differences in hardness and cell proliferation were subjected

and Western blotting and ELISA were employed to detect the expression of autophagy markers

α-SMA and CollagenⅠin the HFBs after the treatment with autophagy inhibitor 3-MA and inducer rapamycin respectively.

Results

The proliferation of HFBs was obviously increased with the increase of SRs hardness (

0.05). Furthermore

the HFBs cultured on harder SRs surface had higher autophagy activity

and lower expression levels of α-SMA and Collagen Ⅰ when compared with the cells on softer SRs surface (

0.01).

Conclusion

The SRs hardness may affect the proliferation of HFBs by regulating autophagy. High hardness SRs promotes the proliferation of HFBs and inhibits the expression of α-SMA and CollagenⅠby enhancing autophagy activity.

关键词

Keywords

references

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