Effects of fluoxetine on cholinergic neurons in dentate gyrus of 8-month-old APP/PS1 mice: a stereological study

Yongxin WU; Chunni ZHOU; Jinhua FAN; Lin JIANG; Yi ZHANG; Lei ZHANG; Fenglei CHAO; Jing MA; Xin LIANG; Yingqiang QI; Jing TANG; Chunmao YANG; Yong TANG

doi:10.16016/j.1000-5404.201811045

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Effects of fluoxetine on cholinergic neurons in dentate gyrus of 8-month-old APP/PS1 mice: a stereological study

更新时间：2026-03-02

- Effects of fluoxetine on cholinergic neurons in dentate gyrus of 8-month-old APP/PS1 mice: a stereological study
- Journal of Third Military Medical University Vol. 41, Issue 8, Pages: 786-793(2019)
- 作者机构：
  
  400016 重庆，重庆医科大学基础医学院人体解剖与组织胚胎学教研室Department of Histology and Embryology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81671259);the General Program of National Natural Science Foundation of China(81501101);the General Project of Basic and Frontier Research Plan of Chongqing(CSTC2015jcyjA10020)
- DOI：10.16016/j.1000-5404.201811045
  CLC： R319;R322.81;R971.43
- Received：07 November 2018，
  
  Published：30 April 2019
- 稿件说明：
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Yongxin WU, Chunni ZHOU, Jinhua FAN, et al. Effects of fluoxetine on cholinergic neurons in dentate gyrus of 8-month-old APP/PS1 mice: a stereological study[J]. Journal of Third Military Medical University, 2019, 41(8): 786-793.
DOI：

Yongxin WU, Chunni ZHOU, Jinhua FAN, et al. Effects of fluoxetine on cholinergic neurons in dentate gyrus of 8-month-old APP/PS1 mice: a stereological study[J]. Journal of Third Military Medical University, 2019, 41(8): 786-793. DOI： 10.16016/j.1000-5404.201811045.

摘要

目的

探讨给予氟西汀(fluoxetine

FLX)干预后，8月龄APP/PS1小鼠海马齿状回(dentate gyrus，DG)内胆碱能神经元的总数以及密度的变化，分析认知功能的改善是否与FLX干预后DG区内胆碱能神经元数量变化有关。

方法

选用20只8月龄雌性APP/PS1小鼠，采用完全随机法分为对照组和干预组，分别给予8周生理盐水和等量FLX腹腔注射后，进行Morris水迷宫测试以检测小鼠学习和记忆能力；运用体视学方法计算两组小鼠海马DG区内胆碱能神经元的总数和密度；运用酶联免疫吸附剂测定胆碱能相关神经递质。

结果

水迷宫实验结果显示，干预组的逃避潜伏期较对照组明显缩短(

0.05)。体视学结果显示，FLX显著增加了APP/PS1小鼠海马DG区内胆碱能神经元的数量和密度(

0.01)；酶联免疫吸附实验结果显示，FLX增加了8月龄APP/PS1小鼠海马乙酰胆碱(acetylcholine

ACh)含量(

0.01)。

结论

海马DG区内胆碱能神经元总数的变化以及海马ACh含量的变化可能与FLX干预后8月龄APP/PS1小鼠行为学的改变有关。

Abstract

Objective

To investigate the change of the total number and density of cholinergic neurons in dentate gyrus (DG) of hippocampus of 8-month-old APP/PS1 mice after fluoxetine (FLX) intervention and analyze whether the improvement of cognitive function is related to the change of the number of cholinergic neurons after FLX intervention.

Methods

Twenty 8-month-old female APP/PS1 mice were randomly divided into control group and intervention group. The mice in the control group and the intervention group were given 8 weeks of normal saline and the same amount of FLX by intraperitoneal injection

Then

Morris water maze test was used to test the learning and memory ability of the mice. The total number and density of the cholinergic neurons in the DG were calculated using stereological methods. The cholinergic-related neurotransmitters were determined by enzyme-linked immunosorbent assay.

Results

The results of Morris water maze test showed that the escape latency of the intervention group was significantly shorter than that of the control group (

0.05). The results of stereological findings showed that FLX significantly increased the total number and density of cholinergic neurons in the DG of hippocampus of 8-month-old APP/PS1 mice (

0.01); The results of enzyme linked immunosorbent assay showed that FLX statistically increased the level of acetylcholine (ACh) in the hippocampus of APP/PS1 mice (

0.01).

Conclusion

The change of the total number of cholinergic neurons in the DG of hippocampus and the level of ACh in the hippocampus may be related to the behavioral changes of 8-month-old APP/PS1 mice after FLX intervention.

关键词

Keywords

references

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