Uridine triphosphate inhibits the proliferation of gastric cancer cells through Ca<sup>2+</sup>-mediated autophagy

Nannan GAO; Hanxing WAN; Hui DONG; Xiaoyan ZHAO

doi:10.16016/j.1000-5404.201811004

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Uridine triphosphate inhibits the proliferation of gastric cancer cells through Ca²⁺-mediated autophagy

更新时间：2026-03-02

- Uridine triphosphate inhibits the proliferation of gastric cancer cells through Ca²⁺-mediated autophagy
- Journal of Third Military Medical University Vol. 41, Issue 8, Pages: 763-769(2019)
- 作者机构：
  
  400037 重庆，陆军军医大学(第三军医大学)第二附属医院消化内科Department of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, China
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201811004
  CLC： R73-361;R730.23;R735.2
- Received：04 November 2018，
  
  Published：30 April 2019
- 稿件说明：
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Nannan GAO, Hanxing WAN, Hui DONG, et al. Uridine triphosphate inhibits the proliferation of gastric cancer cells through Ca²⁺-mediated autophagy[J]. Journal of Third Military Medical University, 2019, 41(8): 763-769.
DOI：

Nannan GAO, Hanxing WAN, Hui DONG, et al. Uridine triphosphate inhibits the proliferation of gastric cancer cells through Ca²⁺-mediated autophagy[J]. Journal of Third Military Medical University, 2019, 41(8): 763-769. DOI： 10.16016/j.1000-5404.201811004.

摘要

目的

观察尿苷三磷酸(uridine triphosphate，UTP)对胃癌细胞增殖的影响，并探讨其分子机制。

方法

采用CCK-8实验检测UTP及加入细胞内钙螯合剂BAPTA-AM后对胃癌细胞SGC-7901和AGS增殖能力的影响；集落形成实验检测UTP对胃癌细胞SGC-7901和AGS集落形成能力的影响；细胞内钙测定实验检测UTP对胃癌细胞SGC-7901和AGS内钙信号的影响；Western blot检测UTP及加入BAPTA-AM后对胃癌细胞SGC-7901和AGS LC3Ⅱ/LC3Ⅰ蛋白表达的影响。

结果

UTP抑制SGC-7901和AGS胃癌细胞的增殖(

0.05)及集落形成能力，此增殖能力可被细胞内钙螯合剂BAPTA-AM逆转；钙测定实验显示UTP可触发胃癌细胞SGC-7901和AGS内的Ca

信号；Western blot检测证实UTP可以上调胃癌细胞SGC-7901和AGS LC3Ⅱ/LC3Ⅰ蛋白表达水平，同时能够被BAPTA-AM阻断(

0.05)。

结论

UTP抑制胃癌细胞的增殖，可能与增加胃癌细胞内钙信号，上调LC3Ⅱ/LC3Ⅰ蛋白表达，进而引起自噬有关。

Abstract

Objective

To study the effect of uridine triphosphate (UTP) on the proliferation of gastric cancer cells and explore the underlying molecular mechanisms.

Methods

The effects of UTP and BAPTA-AM (an intracellular calcium chelator) on the proliferation of gastric cancer cell lines SGC-7901 and AGS were tested using CCK-8 assay. Colony formation assay was used to assess the effect of UTP on the colony-forming ability of gastric cancer cells. The effect of UTP on cyt in the cells was explored with digital Ca

imaging. Western blotting was performed to observe the effect of UTP and BAPTA-AM on the protein expression of LC3Ⅱ/LC3Ⅰ in the cells.

Results

UTP significantly inhibited the proliferation and the colony-forming ability of SGC-7901 and AGS cells(

0.05)

and these inhibitory effects were reversed by BAPTA-AM. Digital Ca

imaging showed that UTP obviously increased cyt in the gastric cancer cells. The results of Western blotting showed that UTP significantly up-regulated the protein expression of LC3Ⅱ/LC3Ⅰ in the cells

and the application of BAPTA-AM obviously blocked the effect of UTP(

0.05).

Conclusion

UTP inhibits the proliferation of gastric cancer cells probably through increasing calcium signaling and up-regulating LC3Ⅱ/LC3Ⅰ

and thus causing autophagy.

关键词

Keywords

references

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Uridine triphosphate inhibits the proliferation of gastric cancer cells through Ca2+-mediated autophagy

DOI：10.16016/j.1000-5404.201811004

摘要

Abstract

关键词

Keywords

references

Uridine triphosphate inhibits the proliferation of gastric cancer cells through Ca²⁺-mediated autophagy