GClnc1 promotes proliferation and migration of human gastric cancer cells <italic style="font-style: italic">in vitro</italic> by activating SIRT3/FOXO3/SOD2 pathway

Liqin LU; Xiaohong FU; Huidong SONG

doi:10.16016/j.1000-5404.201810033

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GClnc1 promotes proliferation and migration of human gastric cancer cells in vitro by activating SIRT3/FOXO3/SOD2 pathway

Basic Medicine | 更新时间：2026-01-09

- GClnc1 promotes proliferation and migration of human gastric cancer cells in vitro by activating SIRT3/FOXO3/SOD2 pathway
- Journal of Third Military Medical University Vol. 41, Issue 7, Pages: 673-677(2019)
- 作者机构：
  
  1.510000 广州，广州市第十二人民医院消化内科
  2.518000 广东深圳，深圳市南山区人民医院肿瘤科
- 作者简介：
- 基金信息：
  
  the Technology R&D and Innovative Design Program of Nanshan District of Shenzhen City(南科研卫2018027号)
- DOI：10.16016/j.1000-5404.201810033
  CLC： R394.2;R730.23;R735.2
- Received：09 October 2018，
  
  Revised：2018-12-10，
  
  Published：15 April 2019
- 稿件说明：
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Liqin LU, Xiaohong FU, Huidong SONG. GClnc1 promotes proliferation and migration of human gastric cancer cells in vitro by activating SIRT3/FOXO3/SOD2 pathway[J]. Journal of Third Military Medical University, 2019, 41(7): 673-677.
DOI：

Liqin LU, Xiaohong FU, Huidong SONG. GClnc1 promotes proliferation and migration of human gastric cancer cells in vitro by activating SIRT3/FOXO3/SOD2 pathway[J]. Journal of Third Military Medical University, 2019, 41(7): 673-677. DOI： 10.16016/j.1000-5404.201810033.

摘要

目的

探究lncRNA GClnc1是否通过SIRT3/FOXO3/SOD2调控胃癌细胞增殖和迁移。

方法

通过转染慢病毒的方法，在人胃癌细胞SGC7901中分别过表达和沉默GClnc1，采用Western blot和qRT-PCR检测SIRT3/FOXO3/SOD2 mRNA和蛋白水平的改变；在过表达GClnc1的同时分别沉默SIRT3/FOXO3/SOD2，采用CCK-8检测细胞增殖能力，划痕实验检测细胞迁移能力。

结果

过表达GClnc1可以上调SIRT3/FOXO3/SOD2的mRNA和蛋白水平(

0.05)，并增强SGC7901细胞的增殖[(1.00±0.14)

(1.79±0.21)

0.05

]

和迁移能力(10.87±0.76

16.53±2.25

0.05)，而沉默GClnc1则降低SIRT3/FOXO3/SOD2的mRNA和蛋白水平(

0.05)，并减少细胞增殖(1.00±0.14

0.62±0.13

0.05)和迁移能力(10.87±0.76

6.46±0.89

0.01)。沉默SIRT3/FOXO3/SOD2可以消除过表达GClnc1对细胞增殖和迁移能力的促进作用。

结论

GClnc1通过激活SIRT3/FOXO3/SOD2信号通路促进SGC7901细胞增殖和迁移。

Abstract

Objective

To investigate whether the long noncoding RNA (lncRNA) GClnc1 regulates the proliferation and migration of gastric cancer cells by modulating the expression of SIRT3/FOXO3/SOD2 pathway.

Methods

We transfected human gastric cancer SGC7901 cells with lentiviral vectors to induce overexpression or knockdown of GClnc1

and examined the changes in the expression of SIRT3/FOXO3/SOD2 at both mRNA and protein levels using qRT-PCR and Western blotting. We also observed the effects of SIRT3/FOXO3/SOD2 silencing on the proliferation and migration of GClnc1-overexpressing SGC7901 cells using CCK8 assay and scratch wound healing assay

respectively.

Results

Overexpression of GClnc1 significantly up-regulated the mRNA and protein levels of SIRT3/FOXO3/SOD2 (

0.05)

and obviously enhanced the proliferation and migration of SGC7901 cells (

0.05). GClnc1 knockdown significantly lowered the the mRNA and protein levels of SIRT3/FOXO3/SOD2 (

0.05) and inhibited the proliferation and migration of the cells (

0.05). Silencing SIRT3/FOXO3/SOD2 obviously eliminated the effect of GClnc1 overexpression on the cell proliferation and migration.

Conclusion

GClnc1 promotes proliferation and migration of SGC7901 cells by activating SIRT3/FOXO3/SOD2 pathway.

关键词

Keywords

references

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GClnc1 promotes proliferation and migration of human gastric cancer cells in vitro by activating SIRT3/FOXO3/SOD2 pathway

DOI：10.16016/j.1000-5404.201810033

摘要

Abstract

关键词

Keywords

references