Association of single nucleotide polymorphism of CDHR3 gene with lower respiratory tract rhinovirus infection in children: analysis of 200 cases

Hui LI; Luo REN; Yao ZHANG; Yiyi YU; Yu GAO; Xin LONG; Enmei LIU

doi:10.16016/j.1000-5404.201809195

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Association of single nucleotide polymorphism of CDHR3 gene with lower respiratory tract rhinovirus infection in children: analysis of 200 cases

Clinical Medicine | 更新时间：2026-01-09

- Association of single nucleotide polymorphism of CDHR3 gene with lower respiratory tract rhinovirus infection in children: analysis of 200 cases
- Journal of Third Military Medical University Vol. 41, Issue 4, Pages: 371-375(2019)
- 作者机构：
  
  1.400014 重庆，重庆医科大学附属儿童医院儿科研究所，儿童发育疾病研究教育部重点实验室，儿童发育重大疾病国家国际科技合作基地，儿童感染免疫重庆市重点实验室
  2.401122 重庆，重庆医科大学附属儿童医院呼吸中心
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201809195
  CLC： R181.32;R394.1;R725.62
- Received：27 September 2018，
  
  Revised：2018-11-14，
  
  Published：28 February 2019
- 稿件说明：
移动端阅览
Hui LI, Luo REN, Yao ZHANG, et al. Association of single nucleotide polymorphism of CDHR3 gene with lower respiratory tract rhinovirus infection in children: analysis of 200 cases[J]. Journal of Third Military Medical University, 2019, 41(4): 371-375.
DOI：

Hui LI, Luo REN, Yao ZHANG, et al. Association of single nucleotide polymorphism of CDHR3 gene with lower respiratory tract rhinovirus infection in children: analysis of 200 cases[J]. Journal of Third Military Medical University, 2019, 41(4): 371-375. DOI： 10.16016/j.1000-5404.201809195.

摘要

目的

探讨CDHR3基因单核苷酸多态性与人鼻病毒(human rhinoviruses

HRV)所致儿童急性下呼吸道感染的关系。

方法

收集2012年1月至2014年11月因HRV所致急性下呼吸道感染的患儿鼻咽抽吸物，提取RNA逆转录后，采用PCR方法扩增CDHR3基因片段，测序后进行rs6967330位点基因分型。利用PCR的方法扩增HRV的VP4基因进行测序分型，实时荧光定量PCR方法进行HRV载量测定，并回顾性分析rs6967330位点与患儿临床特征的相关性。

结果

共纳入200例患儿，其中AA/AG基因型30例(15.0%)，GG基因型170例(85.0%)。HRV所致急性下呼吸道感染患儿中，AA/AG基因型与GG基因型相比更容易出现喘息症状(86.7%

57.1%，

=0.002)，而且更易致重症肺炎(30.0%

11.2%，

=0.018)，一级亲属也更多具有特应性疾病病史(16.7%

5.88%，

=0.039)。Logistic回归分析发现rs6967330 A是HRV感染患儿喘息(

: 4.585，95%

: 1.446 ~ 14.541，

=0.010)及发展为重症病例(

: 3.379，95%

: 1.009 ~ 11.315，

=0.048)的危险因素。而HRV病毒亚型及载量在不同基因型间差异没有统计学意义。

结论

CDHR3基因单核苷酸多态性位点rs6967330可能与HRV感染后的重症及喘息相关。

Abstract

Objective

To explore the association of single nucleotide polymorphism (SNP) at rs6967330 of cadherin-associated family member 3 (CDHR3) gene with acute respiratory tract infection caused by human rhinoviruses (HRVs) in children.

Methods

From January

2012 to November

2014

we collected specimens of nasopharyngeal aspirates from 200 children with acute lower respiratory tract infection caused by HRVs. CDHR3 gene fragment was amplified by PCR from the samples and sequenced for rs6967330 genotyping. The VP4 gene of HRV was amplified with PCR for subsequent sequencing

and real-time fluorescent quantitative PCR was used to estimate the virus load. The correlation between rs6967330 genotypes and the clinical features of the children was analyzed.

Results

Of the 200 children with acute lower respiratory tract infection caused by HRVs

30 (15.0%) had AA/AG genotype and 170 (85.0%) had GG genotype of rs6967330. The children with AA/AG genotype were more likely to have wheezing symptoms than those with GG genotype (86.7%

57.1%

=0.002)

and were also more susceptible to severe pneumonia (30.0%

11.2%

=0.018); the first-degree relatives of the children with AA/AG genotype were also more likely to have a history of atopic diseases(16.7%

5.88%

=0.039). Logistic regression analysis revealed that rs6967330 A was a risk factor for wheezing in children with HRV infection (

: 4.585

95%

: 1.446~14.541

=0.010) and also for progression into severe cases (

: 3.379

95%

: 1.009~11.315

=0.048). No significant differences in HRV subtypes or viral loads were found between the children with different genotypes of rs6967330.

Conclusion

The SNP of CDHR3 gene at rs6967330 may be associated with severe cases and wheezing after lower respiratory tract infection with HRV in children.

关键词

Keywords

references

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