Store-operated calcium entry is involved in proliferation and migration of vascular endothelial cells

Lianyou WANG; Jie YANG; Lan HUANG

doi:10.16016/j.1000-5404.201809115

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Store-operated calcium entry is involved in proliferation and migration of vascular endothelial cells

Basic Medicine | 更新时间：2026-01-09

- Store-operated calcium entry is involved in proliferation and migration of vascular endothelial cells
- Journal of Third Military Medical University Vol. 41, Issue 5, Pages: 424-429(2019)
- 作者机构：
  
  1.450042 郑州，解放军第988医院心内科
  2.400037 重庆，陆军军医大学(第三军医大学)第二附属医院心内科
- 作者简介：
- 基金信息：
  
  the Supporting Program of Postdoctoral Innovation Talents of Chongqing(2017-356)
- DOI：10.16016/j.1000-5404.201809115
  CLC： R322.12;R329.28;R348.2
- Received：17 September 2018，
  
  Revised：2018-12-20，
  
  Published：15 March 2019
- 稿件说明：
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Lianyou WANG, Jie YANG, Lan HUANG. Store-operated calcium entry is involved in proliferation and migration of vascular endothelial cells[J]. Journal of Third Military Medical University, 2019, 41(5): 424-429.
DOI：

Lianyou WANG, Jie YANG, Lan HUANG. Store-operated calcium entry is involved in proliferation and migration of vascular endothelial cells[J]. Journal of Third Military Medical University, 2019, 41(5): 424-429. DOI： 10.16016/j.1000-5404.201809115.

摘要

目的

利用体外培养的人脐静脉血管内皮细胞，初步探讨钙库操纵性钙内流(store-operated calcium entry，SOCE)对血管内皮细胞(endothelial cell，EC)功能的影响。

方法

培养于激光共聚焦培养皿的EC随机分为对照组和不同剂量(10、25、50、75、100 μmol/L)SOCE抑制剂(2-APB)干预组。干预组细胞以2-APB干预5 min后，检测各组细胞的SOCE强度(

=10)。培养于6孔板的细胞，根据实验目的，随机分为对照组和不同剂量(50、75、100 μmol/L)的2-APB干预组。2-APB干预24 h后，以CCK-8检测各组EC的增殖功能(

=9)、以Transwell培养法检测各组EC的迁移功能(

=10)。2-APB干预2 h后，以Griess Reagent方法检测各组EC的NO产量(

=6)以及用Western blot方法检测各组EC的eNOS变化(

=3)。

结果

较低剂量的2-APB(10、25 μmol/L)对SOCE有抑制趋势，但无统计学意义(

0.05)，较高剂量的2-APB(50、75、100 μmol/L)能够显著抑制SOCE(

0.05)。采用具有显著抑制作用浓度的2-APB(50、75、100 μmol/L)抑制SOCE后，能够显著抑制EC的增殖能力(与对照组比较，EC的增殖分别下降33.2%、46.3%和61.2%，

0.05)、迁移功能(与对照组比较，分别下降33.5%、54.3%和64.9%，

0.05)、NO产量(与对照组比较，分别下降40.5%、61.9%和73.1%，

0.05)以及eNOS的合成(与对照组比较，75和100 μmol/L组分别下降45.1%和62.4%，

0.05)。

结论

SOCE参与调节EC的增殖和迁移功能，抑制SOCE能引起EC功能异常，此作用可能是通过抑制eNOS的合成实现。

Abstract

Objective

To investigate the effects of store-operated calcium entry (SOCE) on the bio-functions of vascular endothelial cells (ECs) based on human umbilical vein endothelial cells (HUVECs).

Methods

The cultured HUVECs were divided into control group and 2-APB (SOCE inhibitor

75 and 100 μmol/L) intervention groups. The SOCE was mea

sured by the fluorescence intensity of calcium probe in 5 min after 2-APB treatment (

=10). The proliferation (

=9) and migration (

=10) capacities of HUVECs after different doses of 2-APB treatment (50

75 and 100 μmol/L) for 24 h were measured by CCK-8 assay and Transwell assay. In addition

at 2 h after 2-APB treatment

Griess reagent kit was employed to detect the production of NO (

=6)

and Western blotting was used to measure the expression of eNOS (

=3) in different groups.

Results

Low doses of 2-APB (10 and 20 μmol/L) showed an inhibitory effect on SOCE

but the inhibition had no statistical significance (

0.05)

while high doses of 2-APB (50

75 and 100 μmol/L) inhibited SOCE significantly (

0.05). Treatment of 2-APB (50

75 and 100 μmol/L) also suppressed the proliferation (decreased by 33.2%

46.3% and 61.2%

respectively when compared with control cells

all

0.05)

the migration (decreased by 33.5%

54.3% and 64.9%

respectively when compared with control cells

all

0.05)

NO production (decreased by 40.5%

61.9% and 73.1%

respectively when compared with control cells

all

0.05) as well as the synthesis of eNOS (decreased by 45.1% and 62.4% in the 75 and 100 μmol/L treated cells

respectively when compared with control cells

both

0.05) in ECs.

Conclusion

SOCE is involved in the proliferation and migration of ECs

and inhibition of SOCE can induce ECs dysfunction

which may act through suppressing eNOS synthesis.

关键词

Keywords

references

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