Effects of low-intensity pulsed ultrasound at different intensities on intervertebral disc degeneration in mice

Wanling JIANG; Junlan HUANG; Qiaoyan TAN; Ruobin ZHANG; Can LI; Yangli XIE; Fangfang LI; Meng XU; Mi LIU; Xiaolan DU; Lin CHEN

doi:10.16016/j.1000-5404.201809052

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Effects of low-intensity pulsed ultrasound at different intensities on intervertebral disc degeneration in mice

Basic Medicine | 更新时间：2026-01-09

- Effects of low-intensity pulsed ultrasound at different intensities on intervertebral disc degeneration in mice
- Journal of Third Military Medical University Vol. 40, Issue 22, Pages: 2027-2033(2018)
- 作者机构：
  
  1.400042 重庆，陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)：战创伤康复研究室，创伤实验室, 骨代谢与修复中心, 创伤、烧伤与复合伤国家重点实验室
  2.400042 重庆，陆军军医大学(第三军医大学)第三附属医院(野战外科研究所)：康复医学科
- 作者简介：
- 基金信息：
  
  the Major Research Program of Ministry of Science and Technology of China(2014CB942904);the Key Project of Innovation Program in Military Medicine(16CXZ016)
- DOI：10.16016/j.1000-5404.201809052
  CLC： R-332; R454.3; R681.5
- Received：06 September 2018，
  
  Revised：2018-09-18，
  
  Published：30 November 2018
- 稿件说明：
移动端阅览
Wanling JIANG, Junlan HUANG, Qiaoyan TAN, et al. Effects of low-intensity pulsed ultrasound at different intensities on intervertebral disc degeneration in mice[J]. Journal of Third Military Medical University, 2018, 40(22): 2027-2033.
DOI：

Wanling JIANG, Junlan HUANG, Qiaoyan TAN, et al. Effects of low-intensity pulsed ultrasound at different intensities on intervertebral disc degeneration in mice[J]. Journal of Third Military Medical University, 2018, 40(22): 2027-2033. DOI： 10.16016/j.1000-5404.201809052.

摘要

目的

探讨不同强度的低强度脉冲超声波对椎间盘退变的影响，筛选低强度脉冲超声波的最佳治疗强度。

方法

采用3种强度(0.1、0.5、1.0 W/cm

)的低强度脉冲超声波处理大鼠髓核细胞及筛选的最佳强度超声波处理针刺椎间盘模型小鼠；EdU检测髓核细胞增殖，划痕实验检测细胞迁移，定量PCR检测蛋白聚糖、Ⅱ型胶原、波形蛋白等相关基因表达；组织学分析小鼠尾部椎间盘退变程度。

结果

3种强度的低强度脉

冲超声波能促进髓核细胞增殖及迁移，强度为0.5 W/cm

及1.0 W/cm

的低强度脉冲超声波作用较明显(

0.01)。低强度脉冲超声波也可上调正常环境及炎症刺激下髓核细胞中蛋白聚糖、波形蛋白等表达，其中强度为0.5 W/cm

、频率为3 MHz、占空比为50%中强度的低强度脉冲超声波作用最强(

0.01)。对照组小鼠椎间盘表现出明显退变表型，而超声组椎间盘退变程度的组织评分明显低于对照组(

0.01)。

结论

低强度脉冲超声波可能通过上调椎间盘的蛋白聚糖、波形蛋白、Ⅱ型胶原表达，加快细胞增殖及迁移等，对椎间盘退变有一定防治作用，其中强度为0.5 W/cm

、频率为3 MHz、占空比为50%中强度的低强度脉冲超声波作用最明显。

Abstract

Objective

To investigate the effect of low-intensity pulsed ultrasound (LIPUS) at different intensities on intervertebral disc degeneration (IDD) and determine the optimal therapeutic intensity.

Methods

LIPUS at the intensities of 0.1

0.5 and 1.0 W/cm

were applied on rat nucleus pulposus (NP) cells

and the optimal therapeutic intensity LIPUS was performed in a mouse model of intervertebral disc punctures. The cell proliferation and migration were detected by EdU and wound healing assays

respectively

and the expression levels of the related genes were examined using real-time quantitative PCR. Histological analysis was performed to evaluate the degeneration of the intervertebral discs in the mouse model after LIPUS treatment.

Results

LIPUS treatments at the 3 intensities all promoted the proliferation and migration of rat NP cells

but the effect was stronger with LIPUS intensities of 0.5 and 1.0 W/cm

(

0.01). The treatments up-regulated the mRNA expression levels of Aggrecan and Vimentin in rat NP cells

and the effect was the most obvious at the intensity of 0.5 W/cm

(frequency of 3 MHz with a duty cycle of 50%;

0.01). In LIPUS-treated mouse models of disc punctures

histological scoring showed a significantly lower score for IDD than that in untreated mice (

0.01).

Conclusion

LIPUS can ameliorate IDD by up-regulating the expressions of Aggrecan

Vimentin and type Ⅱ collagen to promote the proliferation and migration of NP cells. An intensity of 0.5 W/cm

a frequency of 3 MHz and a duty cycle of 50% can be the optimal LIPUS setting for treatment of IDD.

关键词

Keywords

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