Effect of LncRNA LINC00475 on biological behaviors of glioma cells

Delong ZHANG; Huanggui ZHOU; Jun LIU; Jie LIU; Jie MAO

doi:10.16016/j.1000-5404.201809037

您当前的位置：

首页 >

文章列表页 >

Effect of LncRNA LINC00475 on biological behaviors of glioma cells

更新时间：2026-02-12

- Effect of LncRNA LINC00475 on biological behaviors of glioma cells
- Journal of Third Military Medical University Vol. 41, Issue 3, Pages: 213-218(2019)
- 作者机构：
  
  241000 安徽芜湖，皖南医学院第一附属医院神经外科Department of Neurosurgery, First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui Province, 241000, China
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science foundation of China(81572486)
- DOI：10.16016/j.1000-5404.201809037
  CLC： R394.3;R730.23;R730.264
- Received：05 September 2018，
  
  Published：15 February 2019
- 稿件说明：
移动端阅览
Delong ZHANG, Huanggui ZHOU, Jun LIU, et al. Effect of LncRNA LINC00475 on biological behaviors of glioma cells[J]. Journal of Third Military Medical University, 2019, 41(3): 213-218.
DOI：

Delong ZHANG, Huanggui ZHOU, Jun LIU, et al. Effect of LncRNA LINC00475 on biological behaviors of glioma cells[J]. Journal of Third Military Medical University, 2019, 41(3): 213-218. DOI： 10.16016/j.1000-5404.201809037.

摘要

目的

探讨LINC00475在不同WHO分级胶质瘤标本中的表达变化，并在胶质瘤细胞系LN382 U87MG中验证LINC00475对增殖、迁移、侵袭功能的影响。

方法

利用TCGA胶质瘤数据库对LINC00475进行差异表达分析、ROC曲线分析和生存分析。采用qRT-PCR技术检测LINC00475在本院临床搜集的胶质瘤样本中的表达，分析LINC00475是否存在差异表达以及与肿瘤分期分级有无相关性，与患者预后是否相关。分别在LN382和U87MG细胞系上采用RTCA实验检测NC组(阴性对照)和silence组(敲减LINC00475)增殖、侵袭、迁移功能的改变。

结果

TCGA数据库提示LINC00475在肿瘤组织中表达显著上调(

0.001)，且ROC曲线下面积等于0.98，与患者预后显著负相关(

0.01)。qRT-PCR结果证实LINC00475在胶质瘤组织(2.62±0.89)中的表达显著高于正常组织(1.00±0.36)，且与肿瘤分期、分级呈正相关(WHO Ⅱ：1.60±0.40，WHO Ⅲ：2.65±0.63

WHO Ⅳ：3.30±0.58)。LINC00475高表达组患者总体生存率显著低于低表达组(

0.05)。两个细胞系的silence组增殖、侵袭、迁移能力显著低于NC组(

0.01)。

结论

LINC00475表达在胶质瘤组织中显著上调，且和WHO分级正相关；高表达的LINC00475促进了胶质瘤细胞增殖、侵袭、迁移能力。

Abstract

Objective

To investigate the differential expression of LINC00475 in brain tissues at different WHO grades of glioma

and determine the effect of LINC00475 on the proliferation

migration

and invasion of glioblastoma cell lines LN382 and U87MG.

Methods

With TCGA-GBM database as the analytical foundation

differential expression analysis

ROC curve plotting

and survival analysis were performed towards LINC00475. The expression of LINC00475 in normal brain tissues from 10 patients with brain trauma and glioma tissues of 48 patients was detected with qRT-PCR. The relationships of differential expression of LINC00475 with WHO grades and with prognosis were analyzed. Real time cellular analysis (RTCA) was applied to detect the cell proliferation

invasion and migration between the negative control cells and the cells after LINC00475 knockdown in LN382 and U87MG cells.

Results

The analysis of TCGA-GBM database indicated that the expression of LINC00475 was significantly up-regulated in glioma tissues (

0.001)

the area under the ROC curve equaled to 0.98

and there was a remarkably negative correlation between LINC00475 expression and patient prognosis (

0.01). qRT-PCR results demonstrated a much higher level of LINC00475 in the glioma tissues than normal tissues (2.62±0.89

1.00±0.36)

and the expression level was positively correlated with WHO grades (WHO Ⅱ: 1.60±0.40

WHO Ⅲ: 2.65±0.63

WHO Ⅳ: 3.30±0.58). Besides

the patients with high LINC00475 expression possessed significantly lower overall survival rate than those with low expression (

0.05). Moreover

the capacities of cell proliferation

invasion and migration were notably decreased in the silenced cells than the normal cells in on both cell lines (

0.01).

Conclusion

LINC00475 is obviously up-regulated in glioma tissue

and is positively correlated with WHO grades. Its higher expression improves the proliferation

invasion and migration capacities in the tumor cells.

关键词

Keywords

references

WEN P Y, HUSE J T. 2016 World Health Organization Classification of central nervous system tumors[J]. Continuum (Minneap Minn), 2017, 23(6): 1531-1547. DOI:10.1212/con.0000000000000536.

DELGADO-L-PEZ P D, CORRALES-GARC-A E M. Survival in glioblastoma: a review on the impact of treatment modalities[J]. Clin Transl Oncol, 2016, 18(11): 1062-1071. DOI:10.1007/s12094-016-1497-x.

OHGAKI H, KLEIHUES P. The definition of primary and secondary glioblastoma[J]. Clin Cancer Res, 2013, 19(4): 764-772. DOI:10.1158/1078-0432.CCR-12-3002.

HEGI M E, DISERENS A C, GODARD S, et al. Clinical trial substantiates the predictive value of O-6-methylguanine-DNA methyltransferase promoter methylation in glioblastoma patients treated with temozolomide[J]. Clin Cancer Res, 2004, 10(6): 1871-1874. DOI:10.1158/1078-0432.ccr-03-0384.

STUPP R, MASON W P, VAN DEN BENT M J, et al. Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma[J]. N Engl J Med, 2005, 352(10): 987-996. DOI:10.1056/NEJMoa043330.

MANSOURI A, MANSOURI S, HACHEM L D, et al. The role of 5-aminolevulinic acid in enhancing surgery for high-grade glioma, its current boundaries, and future perspectives: A systematic review[J]. Cancer, 2016, 122(16): 2469-2478. DOI:10.1002/cncr.30088.

HADJIPANAYIS C G, VAN MEIR E G. Tumor initiating cells in malignant gliomas: biology and implications for therapy[J]. J Mol Med, 2009, 87(4): 363-374. DOI:10.1007/s00109-009-0440-9.

GOURLAY J, MOROKOFF A P, LUWOR R B, et al. The emergent role of exosomes in glioma[J]. J Clin Neurosci, 2017, 35: 13-23. DOI:10.1016/j.jocn.2016.09.021.

HUNG T, WANG Y, LIN M F, et al. Extensive and coordinated transcription of noncoding RNAs within cell-cycle promoters[J]. Nat Genet, 2011, 43(7): 621-629. DOI:10.1038/ng.848.

LU Y, ZHAO X, LIU Q, et al. lncRNA MIR100HG-derived miR-100 and miR-125b mediate cetuximab resistance via Wnt/β-catenin signaling[J]. Nat Med, 2017, 23(11): 1331-1341. DOI:10.1038/nm.4424.

WANG P, LIU Y H, YAO Y L, et al. Long non-coding RNA CASC2 suppresses malignancy in human gliomas by miR-21[J]. Cell Signal, 2015, 27(2): 275-282. DOI:10.1016/j.cellsig.2014.11.011.

LIU Y, ZHENG L, WANG Q, et al. Emerging roles and mechanisms of long noncoding RNAs in atherosclerosis[J]. Int J Cardiol, 2017, 228: 570-582. DOI:10.1016/j.ijcard.2016.11.182.

ZHANG B, WANG D, JI T F, et al. Overexpression of lncRNA ANRIL up-regulates VEGF expression and promotes angiogenesis of diabetes mellitus combined with cerebral infarction by activating NF-κB signaling pathway in a rat model[J]. Oncotarget, 2017, 8(10): 17347-17359. DOI:10.18632/oncotarget.14468.

YANG L, ZHAO G, WANG F, et al. Hypoxia-regulated miR-146a targets cell adhesion molecule 2 to promote proliferation, migration, and invasion of clear cell renal cell carcinoma[J]. Cell Physiol Biochem, 2018, 49(3): 920-931. DOI:10.1159/000493224.

LU Z, DING L, HONG H, et al. Claudin-7 inhibits human lung cancer cell migration and invasion through ERK/MAPK signaling pathway[J]. Exp Cell Res, 2011, 317(13): 1935-1946. DOI:10.1016/j.yexcr.2011.05.019.

LIU R, QIN X P, ZHUANG Y, et al. Glioblastoma recurrence correlates with NLGN3 levels[J]. Cancer Med, 2018, 7(7): 2848-2859. DOI:10.1002/cam4.1538.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

LncRNA GAS5 inhibits glioma progression through miR-135b-5p/APC axis

Expression of long non-coding RNA SFTA1P and its effect on biological functions in lung squamous cell carcinoma

LINC00294 promotes proliferation, invasion, and migration of breast cancer cells via AKT/mTOR/HIF-1α pathway

Role of long non-coding RNA RP11-288L9.1 in systemic lupus erythematosus and underlying mechanism

LncRNA CASC2 enhances sensitivity of glioma cells to temozolomide by ABCG2

Related Author

ZHANG Jidong

WANG Yutao

JI Jianwen

Weiping WAN

Weijia XIE

Tingting XIA

Ying XIANG

Na WU

Related Institution

Neurological Diseases Center， the Third Affiliated Hospital of Chongqing Medical University

Institute of Respiratory Diseases, Key Laboratory of Respiratory Disease of PLA, Second Affiliated Hospital, Army Medical University (Third Military Medical University)

Department of Epidemiology, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University)

Department of Breast and Thyroid Surgery, the Second Affiliated Hospital of Chongqing Medical University

Department of Dermatology, First Affiliated Hospital, Army Medical University (Third Military Medical University)

⁰