Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues

Lan FENG; Dewei CHEN; Wenxiang GAO; Jian CHEN; Gang XU; Guoji E; Qing WU; Binda SUN; Shu HE; Erlong ZHANG; Yuqi GAO

doi:10.16016/j.1000-5404.201808173

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Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues

Basic Medicine | 更新时间：2026-01-09

- Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues
- Journal of Third Military Medical University Vol. 41, Issue 6, Pages: 570-574(2019)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)高原军事医学系：高原特需药品与器材研究室
  2.400038 重庆，陆军军医大学(第三军医大学)高原军事医学系：全军高原医学重点实验室
  3.400038 重庆，陆军军医大学(第三军医大学)高原军事医学系：病理生理学教研室
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Yung Scholars of China(81601640)
- DOI：10.16016/j.1000-5404.201808173
  CLC： R329-33;R361.3;R364.4
- Received：27 August 2018，
  
  Revised：2018-10-29，
  
  Published：30 March 2019
- 稿件说明：
移动端阅览
Lan FENG, Dewei CHEN, Wenxiang GAO, et al. Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues[J]. Journal of Third Military Medical University, 2019, 41(6): 570-574.
DOI：

Lan FENG, Dewei CHEN, Wenxiang GAO, et al. Evaluation of hypoxic injuries in 7 cell lines derived from different human tissues[J]. Journal of Third Military Medical University, 2019, 41(6): 570-574. DOI： 10.16016/j.1000-5404.201808173.

摘要

目的

观察缺氧条件下7种不同类型细胞损伤程度，筛选对缺氧损伤敏感的细胞模型。

方法

选择CCD 841 CoN、GES-1、HUVEC、HEB、HEK-293、HBE和L02等7种不同组织来源的细胞，利用低氧工作站构建不同缺氧浓度(5%O

和1%O

)细胞缺氧模型，使用CCK-8和LDH方法检测细胞增殖及损伤情况。并用红景天(0.625 μmol/L、2.5 mmol/L)和丁苯酞(1、2 μmol/L)于缺氧开始时处理细胞，观察是否可改善缺氧细胞损伤。

结果

与21%O

相比，1%O

处理24 h时，HEB细胞增殖能力显著降低(

0.05)；与21%O

相比，5%O

与1%O

处理24 h HEB、HUVEC、HBE、HEK-293、CCD 841 CoN、GES-1和L02细胞其上清中LDH酶活力均显著升高(

0.05，

0.01)；在HEB细胞中，红景天(2.5 mmol/L)和丁苯酞(1 μmol/L)处理可显著逆转低氧诱导的细胞增殖和细胞活力降低(

0.05)；红景天(2.5 mmol/L)和丁苯酞(1 μmol/L)处理可显著逆转低氧诱导的LDH酶活力升高(

0.05，

0.01)。

结论

HEB细胞对缺氧最为敏感，是较好的抗缺氧损伤药物效应研究细胞的模型。

Abstract

Objective

To observe hypoxic injuries in 7 cell lines derived from different human tissues to identify a cell model that is sensitive to acute hypoxic injury.

Methods

Seven cell lines derived from different human tissues

namely CCD 841 CoN

GES-1

HUVEC

HEB

HEK-293

HBE and L02

were exposed to different oxygen concentrations (1% and 5%) for 24 h using a hypoxia workstation. CCK-8 and LDH kits were used to assess the cell proliferation and cell injuries. The cells were then treated with rhodiola (0.625 μmol/L

2.5 mmol/L) and 3-n-butylphthalide (1

2 μmol/L) at the start of hypoxic exposure to assess the protective effects of the drugs against hypoxic cell injuries.

Results

Compared with the cells cultured in normal oxygen concentration (21%)

HEB cells exposed to 1% O

for 24 h showed significantly lowered proliferative activity (

0.05). Hypoxic exposure to 5% or 1% O

for 24 h significantly increased LDH activity in the culture supernatant of HEB

HUVEC

HBE

HEK-293

CCD 841 CoN

GES-1 and L02 cells (

0.05 or 0.01). In HEB cells cultured under hypoxic conditions

treatment with rhodiola and 3-n-butylphthalide significantly promoted the cell proliferation and improved the cell viability (

0.05)

and obviously reversed hypoxia-induced increase in LDH activity (

0.05 or

0.01).

Conclusion

Among the 7 cell lines tested in this study

HEB cells are the most sensitive to hypoxia and can serve as an ideal model for studying drug interventions of hypoxic cell injuries.

关键词

Keywords

references

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