Sub-minimal inhibitory concentration of imipenem enhances biofilm formation of resistant <italic style="font-style: italic">Acinetobacter baumannii</italic> isolates by affecting type Ⅳ pili

Li FAN; Dandan LI; Jing TIAN; Fengjun SUN; Wei FENG; Shiwen ZHOU

doi:10.16016/j.1000-5404.201808141

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Sub-minimal inhibitory concentration of imipenem enhances biofilm formation of resistant Acinetobacter baumannii isolates by affecting type Ⅳ pili

更新时间：2026-01-09

- Sub-minimal inhibitory concentration of imipenem enhances biofilm formation of resistant Acinetobacter baumannii isolates by affecting type Ⅳ pili
- Journal of Third Military Medical University Vol. 40, Issue 21, Pages: 1967-1972(2018)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201808141
  CLC：
- Received：21 August 2018，
  
  Published：15 November 2018
- 稿件说明：
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Li FAN, Dandan LI, Jing TIAN, et al. Sub-minimal inhibitory concentration of imipenem enhances biofilm formation of resistant Acinetobacter baumannii isolates by affecting type Ⅳ pili[J]. Journal of Third Military Medical University, 2018, 40(21): 1967-1972.
DOI：

Li FAN, Dandan LI, Jing TIAN, et al. Sub-minimal inhibitory concentration of imipenem enhances biofilm formation of resistant Acinetobacter baumannii isolates by affecting type Ⅳ pili[J]. Journal of Third Military Medical University, 2018, 40(21): 1967-1972. DOI： 10.16016/j.1000-5404.201808141.

摘要

Abstract

Objective

To investigate the changes in the biofilm formation of imipenem-resistant and sensitive

Acinetobacter baumannii (A. baumannii)

isolates under the sub-minimal inhibitory concentration of imipenem (sub-MIC IMP).

Methods

MIC values were detected by agar plate dilution in both imipenem-resistant and

sensitive

A. baumannii

isolates. The carbapenemase resistance genes were amplified by PCR. After the intervention of sub-MIC IMP

the biofilm formation was detected by micro-well method

the bacterial adhesion was detected by colony plate counting

and the bacterial motility was observed by swimming

and the expression of the bacterial type IV pili gene was detected by quantitative RT-PCR.

Results

The results of carbapenemase resistance genes showed there were 10 strains of IMP-resistant

A. baumannii

carrying OXA-51 gene

9 carrying OXA-23 gene and only 3 IMP-4 gene. Sub-MIC IMP significantly induced the biofilm formation

adhesion and motility of IMP-resistant

A. baumannii

but no such changes were found in above aspects in the IMP-sensitive strains (5 strains exhibited inhibitory effects

3 showed no effect

and 2 presented induction). Enhanced expression level of type Ⅳ pili gene was found in all the strains with sub-MIC IMP induced biofilm formation.

Conclusion

Sub-MIC IMP may significantly enhance the biofilm formation of

A. baumannii

by affecting the Ⅳ pili related adherence and motility.

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references

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⁰

Sub-minimal inhibitory concentration of imipenem enhances biofilm formation of resistant Acinetobacter baumannii isolates by affecting type Ⅳ pili

DOI：10.16016/j.1000-5404.201808141

摘要

Abstract

关键词

Keywords

references